ucn-1028-c and 2--5--dideoxyadenosine

ACTH (1-24) induces cell shape changes in the immunocytes of the bivalve mollusc, Mytilus galloprovincialis. Using computer-assisted microscopic image analysis, we have found that the G protein antagonist suramin sodium, the adenylate cyclase inhibitor 2',5'-dideoxyadenosine, and the protein kinase inhibitor staurosporine inhibit this effect. The highly specific inhibitors H-89 (for protein kinase A) and calphostin C (for protein kinase C) only inhibited partially the morphological alterations. In contrast, the simultaneous action of H-89 and calphostin C completely blocked these changes. The above findings indicate that ACTH (1-24) induces cell shape changes in molluscan immunocytes via adenylate cyclase/cAMP/protein kinase A pathway, as well as the activation of protein kinase C.

Topics: Adenylyl Cyclase Inhibitors; Animals; Bivalvia; Cell Size; Cosyntropin; Dideoxyadenosine; Enzyme Inhibitors; GTP-Binding Proteins; Hemocytes; Hemolymph; Image Processing, Computer-Assisted; Isoquinolines; Microscopy, Phase-Contrast; Naphthalenes; Protein Kinase Inhibitors; Signal Transduction; Staurosporine; Sulfonamides; Suramin