ubiquinone and triazophos

A novel aerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-stain-negative, coccoid to short-rod-shaped bacterial strain, designated YW11(T), was isolated from soil under long-term application of triazophos. The strain was able to hydrolyse triazophos. Strain YW11(T) grew at 15-40 °C (optimum at 28 °C), at pH 5.0-8.0 (optimum at pH 7.5) and with 0-5.0 % (w/v) NaCl (optimum at 0.5 %). The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were C18 : 1ω7c, C16 : 0, C18 : 1 2-OH and C18 : 0. The genomic DNA G+C content of strain YW11(T) was 69.6±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unknown glycolipid and two unknown aminolipids. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain YW11(T) was a member of the genus Roseomonas, and showed the highest sequence similarity to Roseomonas cervicalis KACC 11686(T) (97.9 %) and Roseomonas aestuarii KACC 19645(T) (97.8 %) and then to Roseomonas ludipueritiae KACC 13843(T) (96.9 %). Strain YW11(T) showed low DNA-DNA relatedness with R. cervicalis KACC 11686(T) (32.3±2.9 %), R. aestuarii KACC 16549(T) (28.2±2.6 %) and R. ludipueritiae KACC 13843(T) (30.2±2.6 %). Based on the results of phylogenetic analysis and DNA-DNA hybridization, the whole-cell fatty acid composition as well as biochemical characteristics, strain YW11(T) was clearly distinguished from all recognized species of the genus Roseomonas and should be assigned to a novel species of the genus Roseomonas, for which the name Roseomonas rhizosphaerae sp. nov. is proposed. The type strain is YW11(T) ( = KACC 17225(T) = CCTCC AB2013041(T)).

Topics: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Methylobacteriaceae; Molecular Sequence Data; Nucleic Acid Hybridization; Organothiophosphates; Phylogeny; Pigmentation; Sequence Analysis, DNA; Soil Microbiology; Soil Pollutants; Triazoles; Ubiquinone

A Gram-stain-negative, non-motile, pale yellow, rod-shaped bacterial strain, YW14(T), was isolated from soil and its taxonomic position was investigated by a polyphasic study. Strain YW14(T) did not form nodules on three different legumes, and the nodD and nifH genes were not detected by PCR. Strain YW14(T) contained Q-10 as the predominant ubiquinone. The major cellular fatty acid was C(18 : 1)ω7c. Phylogenetic analyses based on 16S rRNA gene sequences and seven housekeeping gene sequences (recA, atpD, glnII, gyrB, rpoB, dnaK and thrC) showed that strain YW14(T) belonged to the genus Rhizobium. Strain YW14(T) showed 16S rRNA gene sequence similarity of 93.4-97.3% to the type strains of recognized species of the genus Rhizobium. DNA-DNA relatedness between strain YW14(T) and the type strains of Rhizobium sullae IS123(T) and Rhizobium yanglingense CCBAU 71623(T) was 19.6-25.7%, indicating that strain YW14(T) was distinct from them genetically. Strain YW14(T) could also be differentiated from these phylogenetically related species of the genus Rhizobium by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain YW14(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium flavum sp. nov. is proposed. The type strain is YW14(T) ( = KACC 17222(T) = CCTCC AB2013042(T)).

Topics: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fabaceae; Fatty Acids; Genes, Bacterial; Molecular Sequence Data; Nucleic Acid Hybridization; Organothiophosphates; Phylogeny; Pigmentation; Plant Root Nodulation; Rhizobium; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Pollutants; Triazoles; Ubiquinone