ubiquinone has been researched along with prenol* in 2 studies
2 other study(ies) available for ubiquinone and prenol
| Article | Year |
|---|---|
Rapid high performance liquid chromatography-high resolution mass spectrometry methodology for multiple prenol lipids analysis in zebrafish embryos.
The analysis of lipid molecules in living organism is an important step in deciphering metabolic pathways. Recently, the zebrafish has been adopted as a valuable animal model system to perform in vivo metabolomics studies, however limited methodologies and protocols are currently available to investigate zebrafish lipidome and even fewer to analyze specific classes of lipids. Here we present an HPLC-HRMS based method to rapidly measure multiple prenol lipid molecules from zebrafish tissues. In particular, we have optimized our method for concurrent detection of ubiquinones (Coenzyme Q6, Coenzyme Q9, Coenzyme Q10), cholesterol, vitamin E (α-tocopherol), vitamin K1 and vitamin K2. The purpose of this study was to compare different ionization modes, mobile phases and stationary phases in order to optimize lipid molecules separation. After HPLC-HRMS parameters selection, several extraction conditions from zebrafish embryos were evaluated. We assessed our methodology by quantitation of analytical recovery on zebrafish extracts from wild-type or zebrafish mutants (barolo) affected by impaired biosynthesis of ubiquinones. Topics: alpha-Tocopherol; Animals; Cholesterol; Chromatography, High Pressure Liquid; Embryo, Nonmammalian; Hemiterpenes; Mass Spectrometry; Mutation; Pentanols; Ubiquinone; Vitamin E; Vitamin K 1; Vitamin K 2; Zebrafish | 2015 |
Biosynthesis of isoprenoid compounds in cattle filarial parasite Setaria digitata.
The biological significance of isoprenoid compounds such as ubiquinones, prenols and sterols have been well established. The presence and biological function of the two quinones Q6 and Q8 in the cattle filarial parasite Setaria digitata have already been reported. Inhibition of the function of quinone was already shown to be an effective means of controlling the filarial parasite. Detailed investigations of the non-saponifiable lipids from S. digitata using column, thin layer, reverse phase and high performance liquid chromatography showed the presence and formation of isoprenoid compounds such as prenols and sterols, in addition to the two quinones. Blocking of the biosynthesis of these useful compounds may prove to be an additional means of control of filarial parasites. Topics: Animals; Cattle; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Hemiterpenes; Pentanols; Setaria Nematode; Sterols; Ubiquinone | 1994 |