ubiquinone has been researched along with neurosporene* in 1 studies
1 other study(ies) available for ubiquinone and neurosporene
| Article | Year |
|---|---|
Catalytic properties of an expressed and purified higher plant type zeta-carotene desaturase from Capsicum annuum.
The zeta-carotene desaturase from Capsicum annuum (EC 1.14.99.-) was expressed in Escherichia coli, purified and characterized biochemically. The enzyme acts as a monomer with lipophilic quinones as cofactors. Km values for the substrate zeta-carotene or the intermediate neurosporene in the two-step desaturation reaction are almost identical. Product analysis showed that different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers. The application of different geometric isomers as substrates revealed that the zeta-carotene desaturase has no preference for certain isomers and that the nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Topics: Capsicum; Carotenoids; Escherichia coli; Isomerism; Kinetics; Lycopene; Oxidoreductases; Plants, Medicinal; Plastoquinone; Recombinant Proteins; Ubiquinone | 1999 |