ubiquinone and nerolidol

ubiquinone has been researched along with nerolidol* in 2 studies

Other Studies

2 other study(ies) available for ubiquinone and nerolidol

ArticleYear
Antileishmanial activity of the terpene nerolidol.
    Antimicrobial agents and chemotherapy, 2005, Volume: 49, Issue:5

    The activity of nerolidol, a sesquiterpene used as a food-flavoring agent and currently under testing as a skin penetration enhancer for the transdermal delivery of therapeutic drugs, was evaluated against Leishmania species. Nerolidol inhibited the growth of Leishmania amazonensis, L. braziliensis, and L. chagasi promastigotes and L. amazonensis amastigotes with in vitro 50% inhibitory concentrations of 85, 74, 75, and 67 microM, respectively. The treatment of L. amazonensis-infected macrophages with 100 microM nerolidol resulted in 95% reduction in infection rates. Inhibition of isoprenoid biosynthesis, as shown by reduced incorporation of [2-(14)C]mevalonic acid (MVA) or [1-(14)C]acetic acid precursors into dolichol, ergosterol, and ubiquinone, was observed in nerolidol-treated promastigotes. This drug effect can be attributed to the blockage of an early step in the mevalonate pathway, since incorporation of the precursor [1(n)-(3)H]farnesyl pyrophosphate in polyisoprenoids is not inhibited by nerolidol. L. amazonensis-infected BALB/c mice were treated with intraperitoneal doses of 100 mg/kg/day for 12 days or topically with 5 or 10% ointments for 4 weeks. Significant reduction of lesion sizes in nerolidol treated mice was observed for both treatment routes. However, long-term follow up indicated that the disease was not cured in this highly susceptible animal model. Nonetheless, the in vitro activity of nerolidol against these parasites may prove a useful tool for the development of new drugs for the treatment of leishmaniasis. In addition, biosynthesis of dolichols with 11 and 12 isoprene units was identified in Leishmania, as described for other trypanosomatids and Apicomplexa.

    Topics: Animals; Antiprotozoal Agents; Cell Proliferation; Cell Survival; Cells, Cultured; Chromatography, Thin Layer; Dolichols; Ergosterol; Female; Humans; Leishmania mexicana; Leishmaniasis; Lipid Metabolism; Mice; Mice, Inbred BALB C; Sesquiterpenes; Spectrometry, Mass, Electrospray Ionization; Tail; Ubiquinone

2005
Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum.
    FEMS microbiology letters, 2002, Jan-22, Volume: 207, Issue:1

    Little is known about isoprenoid biosynthesis in parasitic protozoa. The presence of dolichol and isoprenylated proteins has been detected in Plasmodium falciparum, but no studies are available about the biosynthesis of the isoprenic side chain attached to the benzoquinone ring of coenzyme Q. In the present study, using metabolic labelling with different intermediates, we demonstrated the presence of an active isoprenoid pathway for the biosynthesis of the isoprenic chain of coenzyme Q. Our results show that P. falciparum is able to synthesize different homologs (coenzyme Q(8) and coenzyme Q(9)), depending on the given intermediate. Parasites treated with nerolidol at doses 2.2 times below the IC(50) showed a decreased ability to synthesize the isoprenic chain attached to coenzyme Q at all intraerythrocytic stages. Treatment with nerolidol arrested development of the intraerythrocytic stages of the parasites, indicating that the drug may have an antimalarial potential.

    Topics: Animals; Carbon Radioisotopes; Erythrocytes; Humans; Malaria, Falciparum; Parasitic Sensitivity Tests; Plasmodium falciparum; Polyisoprenyl Phosphates; Protein Prenylation; Sesquiterpenes; Tritium; Ubiquinone

2002