ubiquinone and dimethyl-sulfide

A novel restricted facultatively methylotrophic marine strain, MP(T), possessing the ribulose monophosphate pathway of C(1)-carbon compound assimilation was isolated from a seawater sample obtained from Mokpo, South Korea. The novel isolate is aerobic, Gram-negative, asporogenous and a non-motile short rod. It grows well on methanol, methylated amines, dimethylsulfide and DMSO. Optimal growth occurs with 3 % NaCl at 30 degrees C and pH 7.0. Fructose is utilized as a multicarbon source. Growth factors are not required and vitamin B(12) does not stimulate growth. The cellular fatty acid profile of the novel strain consists primarily of straight-chain saturated C(16 : 0) and unsaturated C(16 : 1) acids. The major ubiquinone is Q-8. The dominant phospholipids are phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content is 44.9 mol% (T(m)). Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness (25-41 %) with the type strains of marine methylotrophs belonging to the genus Methylophaga, it is suggested that isolate MP(T) represents a novel species, Methylophaga aminisulfidivorans sp. nov. (type strain MP(T)=KCTC 12909(T)=VKM B-2441(T)=JCM 14647(T)).

Topics: Aerobiosis; Bacterial Typing Techniques; Base Composition; Carbon; Dimethyl Sulfoxide; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Fructose; Genes, rRNA; Hydrogen-Ion Concentration; Korea; Methanol; Methylamines; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids; Phylogeny; Piscirickettsiaceae; RNA, Bacterial; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sodium Chloride; Sulfides; Temperature; Ubiquinone; Vitamin B 12