u-0126 and tyrphostin-25

Recent studies have demonstrated that perivascular adipose tissue (PVAT) releases vascular relaxation factor(s). In this study, we examined if PVAT releases other vasoactive factors in response to perivascular nerve activation by electrical field stimulation (EFS).. In Wistar-Kyoto rats, rings of superior mesenteric artery (MA) with intact PVAT (PVAT (+)) showed a greater contractile response to EFS than rings with PVAT removed (PVAT (-)). Superoxide dismutase (SOD) reduced the contractile response to EFS more in PVAT (+) MA than in PVAT (-) MA. Inhibitors of NAD(P)H oxidase and cyclooxygenase exerted a greater inhibition on EFS-induced contraction in PVAT (+) MA than in PVAT (-) MA. Inhibitors of tyrosine kinase (tyrphostin A25) and MAPK/ERK (U 0126) attenuated EFS-induced contraction in PVAT (+) MA in a concentration-related manner, while inactive forms of these inhibitors (tyrphostin A1 and U 0124) did not inhibit the response. Exogenous superoxide augmented the contractile response to EFS and to phenylephrine in PVAT (-) MA, and this augmentation was blunted by inhibition of tyrosine kinase and MAPK/ERK. EFS increased superoxide generation in isolated PVAT and PVAT (+)/(-) MA, which was attenuated by NAD(P)H oxidase inhibition. RT-PCR showed the mRNA expression of p(67phox) subunit of NAD(P)H oxidase and immunohistochemical staining confirmed its localization in the adipocytes of PVAT.. These results show that PVAT enhances the arterial contractile response to perivascular nerve stimulation through the production of superoxide mediated by NAD(P)H oxidase, and that this enhancement involves activation of tyrosine kinase and MAPK/ERK pathway.

Topics: Adipose Tissue; Angiotensin II; Animals; Butadienes; Cyclooxygenase Inhibitors; Dose-Response Relationship, Drug; Electric Stimulation; Enzyme Inhibitors; Immunohistochemistry; In Vitro Techniques; Male; Mesenteric Arteries; Mitogen-Activated Protein Kinases; Muscle, Smooth, Vascular; NADH, NADPH Oxidoreductases; NADPH Oxidases; NG-Nitroarginine Methyl Ester; Nitric Oxide Synthase; Nitriles; Phenylephrine; Protein-Tyrosine Kinases; Rats; Rats, Inbred SHR; Reverse Transcriptase Polymerase Chain Reaction; Superoxide Dismutase; Superoxides; Tyrphostins; Vasoconstriction