u-0126 and trans-10-cis-12-conjugated-linoleic-acid

u-0126 has been researched along with trans-10-cis-12-conjugated-linoleic-acid* in 1 studies

Other Studies

1 other study(ies) available for u-0126 and trans-10-cis-12-conjugated-linoleic-acid

Article	Year
trans-10, cis-12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes. Molecular reproduction and development, 2014, Volume: 81, Issue:1 The aim of this study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) supplementation on oocyte maturation and embryo development in pigs. Compared with the control, supplementation of 50 µM t10c12 CLA to in vitro maturation (IVM) medium significantly increased the proportion of oocytes at the metaphase-II (MII) stage and subsequent parthenogenetic embryo development in terms of cleavage rate, blastocyst formation rate, and cell numbers in blastocysts. The t10c12 CLA-treated oocytes resumed meiotic maturation and progressed to the MII stage significantly faster than those of control. The expression of phosphorylated mitogen-activated protein kinase 3/1 (p-MAPK3/1) and cyclooxygenase-2 (COX2) in cumulus oocyte complexes (COCs) at 5, 10, and 22 hr of IVM were significantly increased in the t10c12 CLA-treatment group. The level of p-MAPK3/1 in t10c12 CLA-treated MII oocytes was also higher (P < 0.05) than that of control. Moreover, t10c12 CLA supplementation partially overcame the negative effects of U0126 on cumulus expansion and nuclear maturation, and completely recovered COX2 protein levels in the presence of U0126. Treatment of COCs with NS398 also significantly suppressed cumulus expansion and nuclear maturation, which was overcome by t10c12 CLA. Yet, this simulatory effect of t10c12 CLA was blocked in the presence of both U0126 and NS398. The t10c12 CLA treatment significantly reduced reactive oxygen species level and increased glutathione concentrations in MII oocyte. In conclusion, supplementation of t10c12 CLA during porcine oocyte maturation exerts its beneficial effects on nuclear and cytoplasmic maturation, which contributes to enhancing subsequent embryo development. Topics: Animals; Blastocyst; Butadienes; Cell Nucleus; Cyclooxygenase 2; Embryonic Development; Enzyme Activation; Female; Linoleic Acids, Conjugated; Meiosis; Mitogen-Activated Protein Kinase 3; Nitriles; Nitrobenzenes; Oocytes; Parthenogenesis; Reactive Oxygen Species; Sulfonamides; Sus scrofa	2014

Article

Year

trans-10, cis-12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes.

Molecular reproduction and development, 2014, Volume: 81, Issue:1

The aim of this study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) supplementation on oocyte maturation and embryo development in pigs. Compared with the control, supplementation of 50 µM t10c12 CLA to in vitro maturation (IVM) medium significantly increased the proportion of oocytes at the metaphase-II (MII) stage and subsequent parthenogenetic embryo development in terms of cleavage rate, blastocyst formation rate, and cell numbers in blastocysts. The t10c12 CLA-treated oocytes resumed meiotic maturation and progressed to the MII stage significantly faster than those of control. The expression of phosphorylated mitogen-activated protein kinase 3/1 (p-MAPK3/1) and cyclooxygenase-2 (COX2) in cumulus oocyte complexes (COCs) at 5, 10, and 22 hr of IVM were significantly increased in the t10c12 CLA-treatment group. The level of p-MAPK3/1 in t10c12 CLA-treated MII oocytes was also higher (P < 0.05) than that of control. Moreover, t10c12 CLA supplementation partially overcame the negative effects of U0126 on cumulus expansion and nuclear maturation, and completely recovered COX2 protein levels in the presence of U0126. Treatment of COCs with NS398 also significantly suppressed cumulus expansion and nuclear maturation, which was overcome by t10c12 CLA. Yet, this simulatory effect of t10c12 CLA was blocked in the presence of both U0126 and NS398. The t10c12 CLA treatment significantly reduced reactive oxygen species level and increased glutathione concentrations in MII oocyte. In conclusion, supplementation of t10c12 CLA during porcine oocyte maturation exerts its beneficial effects on nuclear and cytoplasmic maturation, which contributes to enhancing subsequent embryo development.

Topics: Animals; Blastocyst; Butadienes; Cell Nucleus; Cyclooxygenase 2; Embryonic Development; Enzyme Activation; Female; Linoleic Acids, Conjugated; Meiosis; Mitogen-Activated Protein Kinase 3; Nitriles; Nitrobenzenes; Oocytes; Parthenogenesis; Reactive Oxygen Species; Sulfonamides; Sus scrofa

2014