u-0126 and pyrrophenone
u-0126 has been researched along with pyrrophenone* in 1 studies
Other Studies
1 other study(ies) available for u-0126 and pyrrophenone
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Galpha13 mediates activation of the cytosolic phospholipase A2alpha through fine regulation of ERK phosphorylation.
Heterotrimeric GTP-binding (G) proteins transduce hormone-induced signals to their effector enzymes, which include several phospholipases. In particular, the G(o)/G(i) and G(q) protein families have been shown to couple signaling to phospholipase A(2) (PLA(2)), phospholipase C, and phospholipase D, while the G(12)/G(13) family has been linked to the activation of small GTPases of the Rho family, and hence, to phospholipase D activation. Here, we demonstrate that in CHO cells, the G(12)/G(13) family is also able to activate cPLA(2)alpha, through the activation of RhoA and, subsequently, ERK1/2. Hormone-induced arachidonic acid release increased as a consequence of Galpha(13) overexpression, and was inhibited through inhibition of Galpha(13) signaling. The Galpha(13)-mediated cPLA(2)alpha activation was inhibited by pharmacological blockade of ERK1/2 with either U0126 or PD98059, and by RhoA inactivation with C3 toxin or a dominant-negative RhoA (N19RhoA), and was stimulated by the serine-threonine phosphatase inhibitor calyculin A. Our data thus identify a pathway of cPLA(2)alpha regulation that is initiated by thrombin and purinergic receptor activation, and that signals through Galpha(13), RhoA and ERK1/2, with the involvement of a calyculin-sensitive phosphatase. Topics: Animals; Arachidonic Acid; Butadienes; CHO Cells; Cricetinae; Cricetulus; Cytosol; Enzyme Activation; Enzyme Inhibitors; Group IV Phospholipases A2; GTP-Binding Protein alpha Subunits, G12-G13; Imidazoles; Marine Toxins; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; Mutation; Nitriles; Oxazoles; Phospholipases A; Phosphoric Monoester Hydrolases; Phosphorylation; Pyridines; Pyrrolidines; rhoA GTP-Binding Protein; Transfection | 2006 |