u-0126 and perfluorooctanoic-acid

u-0126 has been researched along with perfluorooctanoic-acid* in 2 studies

Other Studies

2 other study(ies) available for u-0126 and perfluorooctanoic-acid

ArticleYear
Perfluorooctanoic acid stimulates ovarian cancer cell migration, invasion via ERK/NF-κB/MMP-2/-9 pathway.
    Toxicology letters, 2018, Sep-15, Volume: 294

    As widely used in consumer products, perfluorooctanoic acid (PFOA) has become a common environmental pollutant, which has been detected in human serum and associated with cancers. Our previous study showed that PFOA is a carcinogen that promotes endometrial cancer cell migration and invasion through activation of ERK/mTOR signaling. Here, we showed that PFOA (≥100 nM) treatment also stimulated A2780 ovarian cancer cell invasion and migration, which correlated with increased matrix metalloproteinases MMP-2/-9 expression, important proteases associated with tumor invasion and migration. Notably, PFOA treatment induced activation of ERK1/2/ NF-κB signaling. Pre-treatment with U0126, an ERK1/2inhibitor;or JSH-23, a NF-kB inhibitor, can reverse the PFOA-induced cell migration and invasion. Consistent with these results, inhibiting ERK1/2 or NF-κB signaling abolished PFOA-induced up-regulation of MMP-2/-9 expression. These results indicate that PFOA can stimulate ovarian cancer cell migration, invasion and MMP-2/-9 expression by up-regulating ERK/NF-κB pathway.

    Topics: Active Transport, Cell Nucleus; Apoptosis; Butadienes; Caprylates; Carcinogens, Environmental; Cell Line, Tumor; Cell Movement; Enzyme Induction; Female; Fluorocarbons; Humans; Kinetics; MAP Kinase Signaling System; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Neoplasm Invasiveness; Neoplasm Proteins; NF-kappa B; Nitriles; Ovarian Neoplasms; Phenylenediamines; Phosphorylation; Protein Kinase Inhibitors; Protein Processing, Post-Translational

2018
Perfluorinated chemicals, PFOS and PFOA, enhance the estrogenic effects of 17β-estradiol in T47D human breast cancer cells.
    Journal of applied toxicology : JAT, 2016, Volume: 36, Issue:6

    Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) are the two most popular surfactants among perfluorinated compounds (PFCs), with a wide range of uses. Growing evidence suggests that PFCs have the potential to interfere with estrogen homeostasis, posing a risk of endocrine-disrupting effects. This in vitro study aimed to investigate the estrogenic effect of these compounds on T47D hormone-dependent breast cancer cells. PFOS and PFOA (10(-12) to 10(-4)  M) were not able to induce estrogen response element (ERE) activation in the ERE luciferase reporter assay. The ERE activation was induced when the cells were co-incubated with PFOS (10(-10) to 10(-7)  M) or PFOA (10(-9) to 10(-7)  M) and 1 nM of 17β-estradiol (E2). PFOS and PFOA did not modulate the expression of estrogen-responsive genes, including progesterone (PR) and trefoil factor (pS2), but these compounds enhanced the effect of E2-induced pS2 gene expression. Neither PFOS nor PFOA affected T47D cell viability at any of the tested concentrations. In contrast, co-exposure with PFOS or PFOA and E2 resulted in an increase of E2-induced cell viability, but no effect was found with 10 ng ml(-1) EGF co-exposure. Both compounds also intensified E2-dependent growth in the proliferation assay. ERK1/2 phosphorylation was increased by co-exposure with PFOS or PFOA and E2, but not with EGF. Collectively, this study shows that PFOS and PFOA did not possess estrogenic activity, but they enhanced the effects of E2 on estrogen-responsive gene expression, ERK1/2 activation and the growth of the hormone-deprived T47D cells. Copyright © 2015 John Wiley & Sons, Ltd.

    Topics: Alkanesulfonic Acids; Breast Neoplasms; Butadienes; Caprylates; Carcinogens, Environmental; Cell Line, Tumor; Cell Proliferation; Cell Survival; Endocrine Disruptors; Estradiol; Estrogens; Female; Fluorocarbons; Gene Expression Regulation, Neoplastic; Genes, Reporter; Humans; MAP Kinase Signaling System; Neoplasm Proteins; Nitriles; Osmolar Concentration; Protein Kinase Inhibitors; Response Elements; Surface-Active Agents; Trefoil Factor-1

2016