u-0126 and ethephon

Mitogen-activated protein kinase (MAPK, EC 2.7.11.24) cascade from several plant species has been shown to be activated during response to abiotic stress. Ethylene plays an important role in fruit tolerance to environmental stress. However, the mechanisms by which MAPK regulates defence systems in fruit and the relationship between MAPK and ethylene remain to be determined.. MAPK inhibitor significantly decreased the chilling tolerance of tomato (Lycopersicon esculentum cv. Lichun) fruit during cold storage. Moreover, decreases in ethylene content, LeACS2 expression and activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS, EC 4.4.1.14) and ACC oxidase (ACO, EC 1.14.17.4) due to MAPK inhibitor occurred within 24 h after cold treatment. Upon treatment with cold and ethephon, the ethylene content, activities of ACS and ACO and expression of LeACS2, LeACO1 and LeMAPK4 increased.. The results showed the regulation of MAPK in ethylene biosynthesis to protect tomato fruit from cold stress. In addition, the participation of LeMAPK4 in cold-induced ethylene biosynthesis in tomato fruit was indicated.

Topics: Amino Acid Oxidoreductases; Butadienes; China; Cold Temperature; Ethylenes; Extracellular Signal-Regulated MAP Kinases; Food Preservation; Food Storage; Fruit; Gene Expression Regulation, Plant; Isoenzymes; Lyases; Nitriles; Organophosphorus Compounds; Plant Growth Regulators; Plant Proteins; Plant Proteins, Dietary; Protein Kinase Inhibitors; Refrigeration; Solanum lycopersicum; Up-Regulation