u-0126 has been researched along with 2-phenyl-4-4-5-5-tetramethylimidazoline-1-oxyl-3-oxide* in 1 studies
1 other study(ies) available for u-0126 and 2-phenyl-4-4-5-5-tetramethylimidazoline-1-oxyl-3-oxide
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PACAP stimulation of maturational gonadotropin secretion in goldfish involves extracellular signal-regulated kinase, but not nitric oxide or guanylate cyclase, signaling.
In goldfish, nitric oxide synthase (NOS) immunoreactivity is present in gonadotropes and extracellular signal-regulated protein kinase (ERK) mediates GnRH stimulation of gonadotropin release and synthesis. In this study, we tested the possible involvement of nitric oxide (NO) and ERK in mediating PACAP-stimulated maturational gonadotropin (GTH-II) release from primary cultures of dispersed goldfish pituitary cells. In static incubation experiments, PACAP-induced GTH-II release was unaffected by two inhibitors of NOS synthase, AGH and 1400W; whereas addition of a NO donor, SNAP, elevated GTH-II secretion. In perifusion experiments, neither NOS inhibitors (AGH, 1400W and 7-Ni) nor NO scavengers (PTIO and rutin hydrate) attenuated the GTH-II response to pulse applications of PACAP. In addition, the GTH-II responses to PACAP and the NO donor SNP were additive while PTIO blocked SNP action. Although dibutyryl cGMP increased GTH-II secretion in static incubation, inhibition of guanylate cyclase (GC), a known down-stream target for NO signaling, did not reduce the GTH-II response to pulse application of PACAP. On the other hand, GTH-II responses to PACAP in perifusion were attenuated in the presence of two inhibitors of ERK kinase (MEK), U 0126 and PD 98059. These results suggest that although increased availability of NO and cGMP can lead to increased GTH-II secretion, MEK/ERK signaling, rather than NOS/NO/GC activation, mediates PACAP action on GTH-II release in goldfish. Topics: Animals; Butadienes; Cells, Cultured; Cyclic GMP; Cyclic N-Oxides; Extracellular Signal-Regulated MAP Kinases; Female; Flavonoids; Free Radical Scavengers; Goldfish; Gonadotropins; Guanylate Cyclase; Imidazoles; Male; Nitric Oxide; Nitric Oxide Donors; Nitriles; Pituitary Adenylate Cyclase-Activating Polypeptide; Pituitary Gland; Signal Transduction | 2010 |