tyrosine-o-sulfate and phenylisothiocyanate

tyrosine-o-sulfate has been researched along with phenylisothiocyanate* in 1 studies

Other Studies

1 other study(ies) available for tyrosine-o-sulfate and phenylisothiocyanate

Article	Year
Identification of tyrosine O-sulfate in proteins by reverse-phase high-performance liquid chromatography: use of base hydrolysis combined with precolumn derivatization using phenyl isothiocyanate. Analytical biochemistry, 1986, Volume: 154, Issue:1 A procedure has been developed for the analysis of tyrosine O-sulfate in proteins. Samples are subjected to base hydrolysis with Ba(OH)2, neutralized with sulfuric acid, and the majority of other amino acids removed by chromatography on Dowex AG 50 X 8. The average recovery of tyrosine O-sulfate from these procedures was 43%. Tyrosine O-sulfate was identified by reverse-phase HPLC as the phenylthiocarbamyl derivative following precolumn derivatization with phenyl isothiocyanate. The method has been applied to bovine fibrinogen giving a tyrosine O-sulfate content ranging from 0.59 to 1.23 mol/mol. These procedures were also shown to be suitable for the analysis of the incorporation of [35S]sulfate into tyrosine O-sulfate residues in proteins by intact cells. Topics: Animals; Barium; Barium Compounds; Cattle; Chromatography, High Pressure Liquid; Fibrinogen; Hydrolysis; Isothiocyanates; Proteins; Thiocyanates; Tyrosine	1986

Article

Year

Identification of tyrosine O-sulfate in proteins by reverse-phase high-performance liquid chromatography: use of base hydrolysis combined with precolumn derivatization using phenyl isothiocyanate.

Analytical biochemistry, 1986, Volume: 154, Issue:1

A procedure has been developed for the analysis of tyrosine O-sulfate in proteins. Samples are subjected to base hydrolysis with Ba(OH)2, neutralized with sulfuric acid, and the majority of other amino acids removed by chromatography on Dowex AG 50 X 8. The average recovery of tyrosine O-sulfate from these procedures was 43%. Tyrosine O-sulfate was identified by reverse-phase HPLC as the phenylthiocarbamyl derivative following precolumn derivatization with phenyl isothiocyanate. The method has been applied to bovine fibrinogen giving a tyrosine O-sulfate content ranging from 0.59 to 1.23 mol/mol. These procedures were also shown to be suitable for the analysis of the incorporation of [35S]sulfate into tyrosine O-sulfate residues in proteins by intact cells.

Topics: Animals; Barium; Barium Compounds; Cattle; Chromatography, High Pressure Liquid; Fibrinogen; Hydrolysis; Isothiocyanates; Proteins; Thiocyanates; Tyrosine

1986