tylosin-b and macrocin

tylosin-b has been researched along with macrocin* in 1 studies

Other Studies

1 other study(ies) available for tylosin-b and macrocin

ArticleYear
Characterization of the Two Methylation Steps Involved in the Biosynthesis of Mycinose in Tylosin.
    Journal of natural products, 2016, 08-26, Volume: 79, Issue:8

    The S-adenosyl-l-methionine-dependent O-methyltransferases TylE and TylF catalyze the last two methylation reactions in the tylosin biosynthetic pathway of Streptomyces fradiae. It has long been known that the TylE-catalyzed C2‴-O-methylation of the 6-deoxy-d-allose bound to demethylmacrocin or demethyllactenocin precedes the TylF-catalyzed C3‴-O-methylation of the d-javose (C2‴-O-methylated 6-deoxy-d-allose) attached to macrocin or lactenocin. This study reveals the unexpected substrate promiscuity of TylE and TylF responsible for the biosynthesis of d-mycinose (C3‴-O-methylated d-javose) in tylosin through the identification of a new minor intermediate 2‴-O-demethyldesmycosin (2; 3‴-methyl-demethyllactenocin), which lacks a 2‴-O-methyl group on the mycinose moiety of desmycosin, along with 2‴-O-demethyltylosin (1; 3‴-methyl-demethylmacrocin) that was previously detected from the S. fradiae mutant containing a mutation in the tylE gene. These results unveil the unique substrate flexibility of TylE and TylF and demonstrate their potential for the engineered biosynthesis of novel glycosylated macrolide derivatives.

    Topics: Anti-Bacterial Agents; Hexoses; Leucomycins; Methylation; Methyltransferases; Molecular Structure; Mutation; S-Adenosylmethionine; Streptomyces; Tylosin

2016