tryptophan has been researched along with guanidine in 285 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 15 (5.26) | 18.7374 |
| 1990's | 102 (35.79) | 18.2507 |
| 2000's | 118 (41.40) | 29.6817 |
| 2010's | 47 (16.49) | 24.3611 |
| 2020's | 3 (1.05) | 2.80 |
| Authors | Studies |
|---|---|
| Martorana, GE; Meucci, E; Miggiano, GA; Mordente, A; Santini, SA | 1 |
| Brand, L; James, E; Stites, W; Wu, PG | 1 |
| Goñi, FM; Rial, E; Viguera, AR | 1 |
| Deléage, G; Di Pietro, A; Divita, G; Gautheron, DC; Roux, B | 1 |
| Hamaguchi, K; Kawata, Y | 1 |
| Bujalowski, W; Lohman, TM | 1 |
| Atkinson, T; Chia, WN; Clarke, AR; Holbrook, JJ; Irons, LI; Smith, CJ | 1 |
| Finazzi Agrò, A; Gratton, E; Mei, G; Rosato, N; Savini, I | 1 |
| Bertazzon, A; Lamblin, A; Tian, GH; Tsong, TY | 1 |
| Hamaguchi, K; Kawata, Y; Okajima, T | 1 |
| Horowitz, PM; Tandon, S | 1 |
| Bras, GL; Deville-Bonne, D; Garel, JR; Teschner, W | 1 |
| Berger, JW; Vanderkooi, JM | 1 |
| D'Onofrio, M; Giganti, MG; Grimaldi, S; Pascale, E; Pozzi, D; Verna, R | 1 |
| Cook, K; Hill, BC; Robinson, NC | 1 |
| Gonnelli, M; Strambini, GB | 4 |
| Bismuto, E; Gratton, E; Irace, G | 1 |
| Chang, GG; Chen, YH; Lee, HJ | 1 |
| Church, FC; Griffith, MJ; Villanueva, GB | 1 |
| Edelhoch, H; Grimaldi, S; Robbins, J | 1 |
| Desmadril, M; Yon, JM | 1 |
| Fukunaga, Y; Narita, K; Sakiyama, F; Tamaoki, H | 1 |
| Assmann, G; Lievens, MJ; Rosseneu, M; Schmitz, G; Van Tornout, P | 1 |
| Devlin, TM; Esfahani, M | 1 |
| Frère, JM; Raquet, X; Vanhove, M | 1 |
| Beechem, JM; Mas, MT; Sherman, MA | 2 |
| Hlodan, R; Pain, RH | 1 |
| Christensen, T; Dupont, C; Frandsen, TP; Honzatko, RB; Lehmbeck, J; Stoffer, B; Svensson, B | 1 |
| Arakawa, T; Elliott, S; Kolvenbach, CG; Narhi, LO; Sachdev, R | 1 |
| Georgiou, G; Horowitz, PM; Ostermeier, M; Valax, P | 1 |
| McClelland, DA; Price, NC; Stevens, L | 1 |
| Merrill, AR; Steer, BA | 2 |
| Desmadril, M; Garcia, P; Minard, P; Yon, JM | 1 |
| Carlsson, U; Freskgård, PO; Jonasson, P; Jonsson, BH; Mårtensson, LG; Svensson, M | 1 |
| Owen, CS; Phillips, CM; Sudhakar, K; Vanderkooi, JM | 1 |
| Gajdusek, DC; Gibbs, CJ; Roller, PP; Safar, J | 1 |
| Harrell, S; Laws, D; Le, H; Lian, C; Matsumura, I; Montez, B; Oldfield, E; Patterson, J; Pearson, J | 1 |
| McDonald, IK; Mitchell, JB; Nandi, CL; Price, SL; Thornton, JM | 1 |
| Charles, IG; Coggins, JR; Cooper, A; Deka, R; Hawkins, AR; Kelly, SM; Moore, JD; Price, NC | 1 |
| Gafni, A; Mersol, JV; Steel, DG | 1 |
| Baracca, A; Parenti Castelli, G; Solaini, G; Strambini, GB | 1 |
| Cass, AE; Pappa, HS | 1 |
| Atkinson, T; Badcoe, IG; Burston, SG; Clarke, AR; Holbrook, JJ; Jackson, GS; Smith, CJ; Staniforth, RA | 1 |
| Dahms, TE; Szabo, AG | 1 |
| Fee, L; Gray, T; Grimsley, G; Pace, CN; Vajdos, F | 1 |
| Höök, M; House-Pompeo, K; Joh, D; Speziale, P; Xu, Y | 1 |
| Bandivadekar, KR; Deshpande, VV | 1 |
| Cashikar, AG; Rao, NM | 2 |
| Markley, JL; Royer, CA; Truckses, DM; Vidugiris, GJ | 1 |
| Cerna, V; Dyson, HJ; Holmgren, A; Jeng, MF; Slaby, I | 1 |
| Balasubramanian, D; Sharma, Y; Uma, L | 1 |
| Eftink, MR; Ionescu, R; Maki, AH; Ramsay, GD; Wong, CY; Wu, JQ | 1 |
| Agarwalla, S; Balaram, P; Gokhale, RS; Santi, DV | 1 |
| Krishnamoorthy, G; Nath, U; Periasamy, N; Swaminathan, R; Udgaonkar, JB | 1 |
| Jameson, DM; Uyeda, K; Watanabe, F | 1 |
| Hamada, S; Moriyama, Y; Ogawa, K; Ohara, M; Takeda, K | 1 |
| Frech, C; Glockshuber, R; Schmid, FX; Wunderlich, M | 1 |
| Houry, WA; Rothwarf, DM; Scheraga, HA; Sendak, RA; Wedemeyer, WJ | 1 |
| Gafni, A; Steel, DG; Subramaniam, V | 1 |
| Rothwarf, DM; Scheraga, HA | 1 |
| Mas, MT; Szpikowska, BK | 1 |
| Burston, SG; Clarke, AR; Craven, CJ; Hosszu, LL; Jackson, GS; Parker, MJ; Spencer, J; Waltho, JP | 1 |
| Ho, C; Pratt, EA; Simplaceanu, V; Sun, ZY | 1 |
| Bowler, BE; Dong, A; Garbin, K; Godbole, S | 1 |
| Kim, H; Kim, JS | 1 |
| Chan, CK; Eaton, WA; Hofrichter, J; Hu, Y; Rousseau, DL; Takahashi, S | 1 |
| Arai, M; Kuwajima, K | 1 |
| Coussons, PJ; Hunt, JV; Jacoby, J; Kelly, SM; McKay, A; Price, NC | 1 |
| Burlakova, AA; Debabov, VG; Kurganov, BI | 1 |
| Hammacher, A; Matthews, JM; Norton, RS; Simpson, RJ; Ward, LD | 1 |
| Bellelli, A; Brunori, M; Santanché, S | 1 |
| Nath, U; Udgaonkar, JB | 1 |
| Ferreira, ST; Gondim, KC; Masuda, H; Maya-Monteiro, C; Oliveira, PL | 1 |
| Bonander, N; Karlsson, BG; Leckner, J; Malmström, BG; Wittung-Stafshede, P | 1 |
| Baltzer, L; Dolphin, GT | 1 |
| O'Neil, KT; Park, SH; Roder, H | 1 |
| Kachel, K; London, E; Pablo, L; Wang, Y | 1 |
| Denhardt, DT; Lackland, H; Ludescher, RD; Rajan, SS | 1 |
| Campbell, ID; Dobson, CM; Guijarro, JI; Morton, CJ; Pitkeathly, M; Plaxco, KW | 1 |
| Chen, W; Kulmacz, RJ; Xiao, G | 1 |
| Brenner, RR; Córsico, B; Garda, HA; Toledo, JD; Tricerri, A | 1 |
| Wittung-Stafshede, P | 1 |
| Ball, LJ; Chung, EW; Dobson, CM; Matagne, A; Radford, SE; Robinson, CV | 1 |
| Blandin, P; Desmadril, M; Garcia, P; Mérola, F; Minard, P; Receveur, V | 1 |
| Dobson, CM; Miranker, A; Morgan, CJ | 1 |
| Eftink, MR; Wong, CY | 1 |
| Bhuyan, AK; Udgaonkar, JB | 1 |
| Burton, RE; Ghaemmaghami, S; Oas, TG; Richardson, JS; Word, JM | 1 |
| Brennan, JD; Hogue, CW; Zheng, L | 1 |
| Clottes, E; Leydier, C; Marcillat, O; Perraut, C; Vial, C | 1 |
| Aleksiev, B; Betzel, C; Dolashka, P; Genov, N; Mancheva, I; Rajashankar, KR | 1 |
| Amrani, A; Azarkan, M; Looze, Y; Nijs, M; Smolders, N; Vincentelli, J; Zerhouni, S | 1 |
| Busch, R; Mellins, ED; Reich, Z; Sloan, V; Zaller, DM | 1 |
| Barone, G; Catanzano, F; D'Auria, S; De Paola, B; Graziano, G; Nucci, R; Rossi, M | 1 |
| Das, BK; Liang, JJ | 1 |
| Biswas, S; Ghosh, R; Sen, PC | 1 |
| Clottes, E; Couthon, F; Ebel, C; Leydier, C; Marcillat, O; Vial, C | 1 |
| Brunet, JE; Gratton, E; Jameson, DM; Lasagna, M | 1 |
| Balaram, H; Balaram, P; Gokhale, RS; Ray, SS | 1 |
| Hahn, JS; Hong, H; Jeong, W; Kim, D; Kim, YR; Shin, HC; Song, NW | 1 |
| Bishop, SM; Kohanski, RA; Ross, JB | 1 |
| Bismuto, E; Irace, G; Nucci, R; Rossi, M | 1 |
| DiNardo, AA; Merrill, AR; Steer, BA | 1 |
| Rousseau, DL; Yeh, SR | 1 |
| Park, SH; Roder, H; Shastry, MC | 1 |
| Masaki, K; Mizuguchi, M; Nitta, K | 1 |
| Biktashev, AG; Khaitlina, SY; Kuznetsova, IM; Turoverov, KK; Uversky, VN; Vassilenko, KS | 1 |
| Brito, RM; Quintas, A; Saraiva, MJ | 1 |
| Manyusa, S; Mortuza, G; Whitford, D | 1 |
| Behere, DV; Deva, MS | 1 |
| Chattopadhyay, K; Mazumdar, S | 1 |
| France, RM; Grossman, SH | 1 |
| Eaton, WA; Hagen, SJ | 1 |
| Guidry, J; Moczygemba, C; Wittung-Stafshede, P | 1 |
| Jutila, A; Kinnunen, PK; Zhu, K | 1 |
| Kallenbach, NR; Taudte, S; Xin, H | 1 |
| Deshpande, VV; Ghatge, MS; Tanksale, AM; Vernekar, JV | 1 |
| Kay, C; Prenner, EJ; Ryan, RO; Weers, PM | 1 |
| Eastman, P; Fink, AL; Nishimura, C; Riley, R | 1 |
| Hodges, RS; Lavigne, P; Mant, CT; Tripet, B; Wagschal, K | 1 |
| Jing, G; Yin, J | 1 |
| Inouye, K; Oneda, H; Tanaka, H | 1 |
| Bowley, SA; Frank, MK; Gronenborn, AM; Knutson, JR; Tcherkasskaya, O | 1 |
| Edwin, F; Jagannadham, MV | 1 |
| Deshpande, VV; Pawar, SA | 1 |
| Haas, E; Ittah, V; Landsman, P; Navon, A; Scheraga, HA | 1 |
| Ribou, AC; Salmon, JM; Viallet, PM; Vigo, J; Vo-Dinh, T | 1 |
| Gee, AC; Katzenellenbogen, JA | 1 |
| Andersson, D; Carlsson, U; Hammarström, P | 1 |
| Xie, ZQ; Xu, GJ; Yuan, C; Zhang, FW | 1 |
| Fischer, CJ; Gafni, A; Schauerte, JA; Steel, DG; Wisser, KC | 1 |
| Khan, MM; Tayyab, S | 1 |
| Bigotti, MG; Brunori, M; Cutruzzolà, F; Gianni, S; Travaglini-Allocatelli, C | 1 |
| Strambini, EG; Strambini, GB | 1 |
| Chen, CH; Martin, DL; Rust, E | 1 |
| Barthe, P; Boyer, M; Guignard, L; Roumestand, C; Royer, CA | 1 |
| Hammarström, P; Jiang, X; Kelly, JW; Petrassi, HM; Sacchettini, JC; Smith, CS; White, JT | 1 |
| Matthews, KS; Nichols, NM | 1 |
| Clérico, EM; Ermácora, MR | 1 |
| Balny, C; Glaser, M; Mantulin, WW; Ruan, K; Ruan, Q | 1 |
| Gollnick, P; Li, PT; Scott, DJ | 1 |
| Chánez-Cárdenas, ME; Coria, R; Fernández-Velasco, DA; Pérez-Montfort, R; Saab-Rincón, G; Vázquez-Contreras, E | 1 |
| Bottomley, SP; Cabrita, LD; Whisstock, JC | 1 |
| Hansen, JE; McBrayer, MK; Robbins, M; Suh, Y | 1 |
| Aisenbrey, C; Bechinger, B; Cusan, M; Sathish, HA | 1 |
| Pal, SK; Peon, J; Zewail, AH | 1 |
| Crowhurst, KA; Forman-Kay, JD; Tollinger, M | 1 |
| Babovic-Vuksanovic, D; Burghardt, TP; Carpenter, JF; Gregersen, N; Kim, YS; Nguyen, TV; Riggs, C; Vockley, J | 1 |
| Jaenicke, R; Kalbitzer, HR; Kremer, W; Schuler, B | 1 |
| Christensen, T; Frandsen, TP; Kaarsholm, NC; Sigurskjold, BW; Svensson, B | 1 |
| Bodenreider, C; Goldberg, ME; Kellershohn, N; Méjean, A | 1 |
| Finke, JM; Jennings, PA | 1 |
| Adschiri, T; Ashish, K; Goda, S; Hara, M; Kumagai, I; Tsumoto, K; Umetsu, M | 1 |
| Balaram, H; Balaram, P; Maithal, K; Pattanaik, P; Ravindra, G; Sengupta, C | 1 |
| Bowler, BE; Smith, CR; Wandschneider, E | 1 |
| Singh, S; Zlotnick, A | 1 |
| Kalonia, DS; Sharma, VK | 1 |
| Anderluh, G; Boetzel, R; Hong, Q; Lakey, JH; MacDonald, C; Moore, GR; Virden, R | 1 |
| Azuaga, AI; Berends, R; Canet, D; Dobson, CM; Duurkens, R; Mateo, PL; Robillard, GT; Scheek, RM; Smeenk, G; Titgemeijer, F; van Nuland, NA | 1 |
| Bakalova, A; Kuciel, R; Mazurkiewicz, A; Wójciak, P | 1 |
| Cavagnero, S; Chow, C; Chow, CC; Huppert, TJ; Kimball, EB; Raghunathan, V | 1 |
| Bisaillon, M; Bougie, I | 1 |
| Breccia, P; de Mendoza, J; Gago, F; Martín-Santamaría, S; Pérez-Fernández, R; Prados, P; Van Gool, M | 1 |
| Banerjee, KK; Chattopadhyay, K | 1 |
| Saigo, S; Shibayama, N | 1 |
| Buscaglia, M; Eaton, WA; Hofrichter, J; Lapidus, LJ; Schuler, B | 1 |
| STEINER, RF | 1 |
| AGRAWAL, HC; PANT, R | 1 |
| Cai, MY; Guo, SY; Li, L; Zhang, HR | 1 |
| Gupta, P; Khan, RH; Saleemuddin, M | 1 |
| Kuznetsova, IM; Mazhul', VM; Shavlovsky, MM; Stepanenko, OV; Turoverov, KK; Zaitseva, EM | 1 |
| Layfield, R; Platt, GW; Searle, MS; Simpson, SA | 1 |
| Kadoya, A; Miyake, H; Ohyashiki, T | 1 |
| Brunet, JE; Jameson, DM; Lagos, R; Monasterio, O; Sánchez, SA | 1 |
| Magg, C; Schmid, FX | 1 |
| Huang, C; Kuznetsova, IM; Stepanenko, OV; Turoverov, KK; Wang, CC | 1 |
| Flaugh, SL; King, J; Kosinski-Collins, MS | 1 |
| Gittis, AG; Lattman, EE; Leila Reynald, R; Nguyen, DM | 1 |
| Allocati, N; Di Ilio, C; Favaloro, B; Federici, L; Masulli, M; Pietracupa, M | 1 |
| Fernández, M; Fuentes, L; Oyola, J; Quiñones, E | 1 |
| Arakawa, T; Kumagai, I; Nakanishi, T; Sakai, N; Tanaka, I; Tanaka, Y; Tsumoto, K; Umetsu, M; Yao, M; Yasutake, Y | 1 |
| Guo, Q; Guo, SY; Wang, X; Zhao, F | 1 |
| Bottomley, SP; Cabrita, LD; Gore, MG; Pearce, MC; Rubin, H | 1 |
| Bofill, R; Crespo, MD; Platt, GW; Searle, MS | 1 |
| Dempsey, CE; Mason, PE; Piggot, TJ | 1 |
| Banci, L; Barker, PD; Bruix, M; Ciofi-Baffoni, S; de Lumley Woodyear, T; Fersht, AR; Garcia, P; Johnson, CM; Ramachandra Shastry, MC; Rico, M; Roder, H | 1 |
| Chen, L; Gui, C; Jiang, H; Jiang, W; Luo, H; Shen, J; Shen, X; Yang, S; Yu, C; Yu, H; Zhang, L | 1 |
| Annapurna, V; Chary, KV; Jeromin, A; Jobby, MK; Krishnan, K; Muralidhar, D; Sharma, Y | 1 |
| Bhattacharyya, M; Chakrabarti, A; Ray, S | 1 |
| Atanasov, B; Dolashka-Angelova, P; Dolashki, A; Hristova, R; Nikolov, P; Stevanovic, S; Voelter, W | 1 |
| Arai, M; Koshiba, T; Kuwajima, K; Maki, K; Nakao, M; Nitta, K | 1 |
| Arakawa, T; Ejima, D; Ishibashi, M; Kita, Y; Tokunaga, M; Tsumoto, K | 1 |
| Li, L; Mandelkow, E; von Bergen, M | 1 |
| Chatani, E; Goto, Y; Kato, M; Kawai, T; Naiki, H | 1 |
| Abel, J; Barnes, JM; Brems, DN; King, J; Maloney, KM; Pipes, G; Raso, SW; Treuheit, MJ | 1 |
| Doose, S; Neuweiler, H; Sauer, M | 1 |
| Chedad, A; Hanssens, I; Van Dael, H; Vanhooren, A | 1 |
| Ahmad, B; Ansari, MA; Khan, RH; Sen, P | 1 |
| Atkinson, D; Chroni, A; Gorshkova, IN; Kan, HY; Liu, T; Zannis, VI | 1 |
| Gonçalves, S; Martins-Silva, J; Saldanha, C; Santos, NC | 1 |
| Ado, K; Kikuchi, M; Takeda, N; Taniguchi, Y | 1 |
| Dhanasekaran, P; Lund-Katz, S; Phillips, MC; Saito, H; Sakamoto, T; Tanaka, M; Vedhachalam, C | 1 |
| Baase, WA; Bischoff, N; Dyer, CM; Matthews, BW; Yousef, MS | 1 |
| Kuznetsova, IM; Mazhul', VM; Povarova, OI; Shavlovskiĭ, MM; Turoverov, KK; Zaĭtseva, EM | 1 |
| Aponte, RA; Bonvin, J; Christendat, D; Marcantonio, M; Singh, S; Turnbull, JL | 1 |
| Fersht, AR; Religa, TL; Sato, S | 1 |
| Chaudhuri, TK; Deb, JK; Jana, S | 1 |
| Ahmad, F; Anjum, F; Dar, TA; Islam, A; Moosavi-Movahedi, AA; Singh, LR | 1 |
| Andreo, CS; Ferreyra, ML; Spampinato, CP | 1 |
| Chen, YH; Lee, HJ; Pon, YC; Yin, FY; Yu, CM | 1 |
| Bhardwaj, A; Cingolani, G; Olia, AS; Walker-Kopp, N | 1 |
| Arbildua, JJ; Brunet, JE; Díaz-Espinoza, R; Garcés, AP; Lagos, R; Monasterio, O; Montecinos, F | 1 |
| Chalovich, JM; Kenney, JM; Khaymina, SS; Schroeter, MM | 1 |
| Chen, Y; Kopka, M; Lapidus, LJ; Singh, VR; Wedemeyer, WJ | 1 |
| D'Auria, S; Fini, C; Kuznetsova, IM; Marabotti, A; Rossi, M; Staiano, M; Stepanenko, OV; Turoverov, KK; Varriale, A | 1 |
| Asenjo, JL; Ludwig, HC; Maureira, MA; Pardo, FN; Slebe, JC; Yañez, AJ | 1 |
| Demirkan, E; Doseeva, V; Galkin, A; Herzberg, O; Krajewski, W; Moult, J; Muthukumar, L; Pullalarevu, S; Ramprakash, J; Schwarz, FP | 1 |
| Alston, RW; Grimsley, GR; Lasagna, M; Pace, CN; Reinhart, GD; Scholtz, JM | 1 |
| Bhakuni, V; Singh, K | 1 |
| Chen, HM; Chow, CY; Fang, HJ; Hu, CK; Tsong, TY; Wu, MC | 1 |
| Kuznetsova, IM; Shavlovsky, MM; Stepanenko, OV; Turoverov, KK; Uversky, VN; Verkhusha, VV | 1 |
| Barber, KR; Bottomley, SP; Ferguson, PL; Marlatt, NM; Shaw, GS | 1 |
| van Amerongen, H; van Hoek, A; van Mierlo, CP; Visser, AJ; Visser, NV; Westphal, AH | 1 |
| Chakrabartty, A; Ho, S; Kerman, A; Mulligan, VK | 1 |
| Bellini, T; Buscaglia, M; Longhi, R; Soranno, A | 1 |
| Jagtap, S; Rao, M | 1 |
| Dwivedi, UN; Goyal, N; Rai, S | 1 |
| O'Shaughnessy, D; Ryder, AG; Togashi, DM | 1 |
| Chen, Y; Lapidus, LJ; Parrini, C; Taddei, N | 1 |
| Best, RB; Eaton, WA; Hofrichter, J; Vaiana, SM; Yau, WM | 1 |
| Fernández-Escamilla, AM; Maqueda, M; Martínez-Bueno, M; Sánchez-Hidalgo, M; Serrano, L; Valdivia, E | 1 |
| Cho, AS; Grothe, HL; Huang, AJ; Little, MR; Yuan, C | 1 |
| Chaudhuri, TK; Gupta, P; Mishra, S | 1 |
| Sharp, KA; Vanderkooi, JM; Zelent, B | 1 |
| Chen, R; Feng, J; Li, Y; Pang, XF; Ren, ZL; Tang, LX; Zhang, T | 1 |
| Chandra, V; Dave, S; Dkhar, HK; Gupta, P; Mahajan, S | 1 |
| Albericio, F; Fernández-Carvajal, A; Ferrer-Montiel, A; García-López, MT; González-Muñiz, R; Pérez de Vega, MJ; Royo, M; Zaccaro, L | 1 |
| Chen, HM; Fang, HJ; Forrest, MD; Hu, CK; Hu, HY; Tsong, TY; Wu, MC | 1 |
| Guchhait, N; Paul, BK; Samanta, A | 1 |
| Chennamsetty, N; Helk, B; Kayser, V; Trout, BL; Voynov, V | 1 |
| Jones, CN; Rho, H; Rose, RB | 1 |
| Baron, B; Chenal, A; England, P; Karst, JC; Ladant, D; Sotomayor Pérez, AC; Wozniak, AK | 1 |
| Wang, SS; Wen, WS | 1 |
| Ainciart, N; Bonomi, HR; Cauerhff, AA; Craig, PO; Goldbaum, FA; Nygaard, D; Zylberman, V | 1 |
| Haran, G; Sherman, E | 1 |
| Chaudhuri, TK; Mishra, S; Shah, MA | 1 |
| Bednárova, L; Heyda, J; Jungwirth, P; Konvalinka, J; Kožíšek, M; Thompson, G; Vondrášek, J | 1 |
| Etezad, SM; Ganjalikhany, MR; Khajeh, K; Khalifeh, K; Moghadam, TT; Ranjbar, B | 1 |
| Chandra, V; Dave, S; Gupta, P; Mahajan, S | 1 |
| Chen, HL; Chen, JC; Lin, LL; Lo, HF; Su, JY | 1 |
| Chen, KC; Roder, H; Wedemeyer, WJ; Xu, M | 1 |
| Bakajin, O; Cellmer, T; Ghosh, K; King, M; Lapidus, LJ; Zhu, L | 1 |
| Bowler, BE; Khan, MK; Miller, AL | 1 |
| Ghosh, G; Mandal, DK | 1 |
| Ho, J; Wong, KF | 1 |
| Aatif, M; Amin, F; Bano, B; Khan, MS; Priyadarshini, M; Shah, A | 1 |
| Dasgupta, A; Udgaonkar, JB | 1 |
| Chakrabarti, P; Chakravarty, D; Chatterjee, T; Dey, S; Pal, A; Saha, RP | 1 |
| Gaikwad, SM; Jaokar, TM; Krishnasastry, MV; Patel, KA; Wakankar, MS | 1 |
| Bowie, D; Duncan, D; Fyles, TM; Nelson, CJ; Parvizi, P | 1 |
| Mandal, DK; Sen, D | 1 |
| Sen, P; Yadav, R | 1 |
| Podile, AR; Swamy, MJ; Tarafdar, PK; Vedantam, LV | 1 |
| Bódis, E; Lukács, A; Majer, Z; Nyitrai, M; Raics, K | 1 |
| Auton, M; Blancas-Mejia, LM; Madde, P; Tischer, A | 1 |
| Jha, SK; Marqusee, S | 1 |
| Cabaleiro-Lago, EM; Campo-Cacharrón, A; Carrazana-García, JA; Rodríguez-Otero, J | 1 |
| Kaur, PK; Rashmi, S; Singh, S; Supin, JS | 1 |
| Cohen, P; Dill, KA; Jaswal, SS | 1 |
| Chen, BE; Chi, MC; Lin, LL; Lin, MG; Lo, HF; Wang, TF | 1 |
| Dhanasekaran, P; Hata, M; Lund-Katz, S; Mizuguchi, C; Nickel, M; Okuhira, K; Phillips, MC; Saito, H | 1 |
| Hamada, D; Ikeguchi, M; Ota, C; Tanaka, A | 1 |
| Hsieh, MC; Huang, CY; Zhou, Q | 1 |
| Datta, D; J Swamy, M | 1 |
| Jamir, K; Seshagirirao, K | 1 |
| Lan, H; Lyu, M; Wen, L; Xiao, H; Yin, Z; Zuo, Z | 1 |
| Basu Roy, M; Ghosh, R; Ghosh, S; Mukherjee, M; Mukherjee, P; Saha Sardar, P | 1 |
| Jiaranaikulwanitch, J; Jiranusornkul, S; Saenjum, C; Vajragupta, O; Wongrattanakamon, P; Yooin, W | 1 |
| Arakawa, T; Nishinami, S; Shiraki, K | 1 |
1 review(s) available for tryptophan and guanidine
| Article | Year |
|---|---|
Is arginine a protein-denaturant?
Topics: Amino Acids, Aromatic; Arginine; Guanidine; Protein Denaturation; Protein Structure, Tertiary; Surface Properties; Tryptophan; Tyrosine | 2005 |
284 other study(ies) available for tryptophan and guanidine
| Article | Year |
|---|---|
Conformational stability of bovine alpha-crystallin. Evidence for a destabilizing effect of ascorbate.
Topics: Acrylamide; Acrylamides; Animals; Ascorbic Acid; Cattle; Crystallins; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; In Vitro Techniques; Iodides; Light; Nephelometry and Turbidimetry; Protein Conformation; Protein Denaturation; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan; Urea | 1992 |
Compact denatured state of a staphylococcal nuclease mutant by guanidinium as determined by resonance energy transfer.
Topics: Amino Acid Sequence; Cysteine; Energy Transfer; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Kinetics; Lysine; Mathematics; Micrococcal Nuclease; Mutagenesis, Site-Directed; Naphthalenesulfonates; Protein Denaturation; Recombinant Proteins; Tryptophan | 1992 |
The uncoupling protein from brown adipose tissue mitochondria. The environment of the tryptophan residues as revealed by quenching of the intrinsic fluorescence.
Topics: Adipose Tissue, Brown; Animals; Carrier Proteins; Cricetinae; Guanidine; Guanidines; Hydrochloric Acid; Ion Channels; Mathematics; Membrane Proteins; Mitochondria; Mitochondrial Proteins; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Uncoupling Protein 1; Urea | 1992 |
Intrinsic tryptophan fluorescence of Schizosaccharomyces pombe mitochondrial F1-ATPase. A powerful probe for phosphate and nucleotide interactions.
Topics: Acrylamide; Acrylamides; Adenosine Diphosphate; Adenosine Triphosphate; Guanidine; Guanidines; Iodides; Macromolecular Substances; Mitochondria; Protein Conformation; Proton-Translocating ATPases; Schizosaccharomyces; Spectrometry, Fluorescence; Tryptophan | 1991 |
Use of fluorescence energy transfer to characterize the compactness of the constant fragment of an immunoglobulin light chain in the early stage of folding.
Topics: Circular Dichroism; Energy Transfer; Fluorescent Dyes; Guanidine; Guanidines; Immunoglobulin Light Chains; Kinetics; Naphthalenesulfonates; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; X-Ray Diffraction | 1991 |
Monomer-tetramer equilibrium of the Escherichia coli ssb-1 mutant single strand binding protein.
Topics: DNA-Binding Proteins; Escherichia coli; Fluorescence Polarization; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Magnesium Chloride; Molecular Structure; Mutation; Osmolar Concentration; Sodium Chloride; Temperature; Thermodynamics; Tryptophan; Urea | 1991 |
Detection and characterization of intermediates in the folding of large proteins by the use of genetically inserted tryptophan probes.
Topics: Amino Acid Sequence; Chromatography, Gel; Circular Dichroism; Enzyme Stability; Geobacillus stearothermophilus; Guanidine; Guanidines; Kinetics; L-Lactate Dehydrogenase; Molecular Probes; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleotide Mapping; Protein Conformation; Tryptophan | 1991 |
Dynamic fluorescence in copper proteins. Selected examples.
Topics: Azurin; Bacterial Proteins; Copper; Guanidine; Guanidines; Humans; Metalloproteins; Spectrometry, Fluorescence; Superoxide Dismutase; Temperature; Tryptophan | 1990 |
Enthalpic and entropic contributions to actin stability: calorimetry, circular dichroism, and fluorescence study and effects of calcium.
Topics: Actins; Animals; Calcium; Calorimetry; Circular Dichroism; Drug Stability; Egtazic Acid; Fluorescence; Guanidine; Guanidines; Hot Temperature; Male; Nucleic Acid Conformation; Protein Denaturation; Rabbits; Thermodynamics; Tryptophan | 1990 |
Chemical modification of tryptophan residues and stability changes in proteins.
Topics: Animals; Chickens; Circular Dichroism; Egg White; Enzyme Stability; Guanidine; Guanidines; Hot Temperature; Immunoglobulins; Kinetics; Muramidase; Ozone; Protein Conformation; Ribonuclease T1; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1990 |
The detection of kinetic intermediate(s) during refolding of rhodanese.
Topics: Animals; Cattle; Guanidine; Guanidines; Kinetics; Mitochondria, Liver; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Sulfurtransferases; Thiosulfate Sulfurtransferase; Time Factors; Tryptophan | 1990 |
Urea-induced inactivation, dissociation, and unfolding of the allosteric phosphofructokinase from Escherichia coli.
Topics: Allosteric Regulation; Chromatography, High Pressure Liquid; Escherichia coli; Guanidine; Guanidines; Kinetics; Phosphofructokinase-1; Protein Conformation; Protein Denaturation; Thiocyanates; Tryptophan; Urea | 1989 |
Characterization of lens alpha-crystallin tryptophan microenvironments by room temperature phosphorescence spectroscopy.
Topics: Acrylamide; Acrylamides; Crystallins; Guanidine; Guanidines; Iodides; Luminescent Measurements; Nitrites; Protein Conformation; Protein Denaturation; Spectrum Analysis; Temperature; Thermodynamics; Tryptophan; Urea | 1989 |
Effect of ouabain binding on the fluorescent properties of the Na+/K+-ATPase.
Topics: Acrylamide; Acrylamides; Animals; Fluorescence; Guanidine; Guanidines; Hydrogen-Ion Concentration; Iodides; Ouabain; Protein Conformation; Sodium-Potassium-Exchanging ATPase; Swine; Tryptophan | 1988 |
Subunit dissociation and protein unfolding in the bovine heart cytochrome oxidase complex induced by guanidine hydrochloride.
Topics: Circular Dichroism; Electron Transport Complex IV; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan | 1988 |
Effects of urea and guanidine hydrochloride on the activity and dynamical structure of equine liver alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Animals; Circular Dichroism; Guanidine; Guanidines; Horses; Kinetics; Liver; Luminescent Measurements; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Urea; Viscosity | 1986 |
Effect of unfolding on the tryptophanyl fluorescence lifetime distribution in apomyoglobin.
Topics: Animals; Apoproteins; Guanidine; Guanidines; Kinetics; Myocardium; Myoglobin; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Tuna | 1988 |
Fluorescence studies on the dissociation and denaturation of pigeon liver malic enzyme.
Topics: Acrylamide; Acrylamides; Algorithms; Animals; Columbidae; Fluorescence; Guanidine; Guanidines; Liver; Malate Dehydrogenase; Molecular Weight; Protein Denaturation; Tryptophan | 1988 |
Structure-function relationships in heparin cofactor II: chemical modification of arginine and tryptophan and demonstration of a two-domain structure.
Topics: Amino Acids; Antithrombin III; Arginine; Binding Sites; Bromosuccinimide; Chromatography; Circular Dichroism; Diacetyl; Glycoproteins; Guanidine; Guanidines; Heparin Cofactor II; Kinetics; Protein Denaturation; Structure-Activity Relationship; Tryptophan | 1986 |
Effects of thyroxine binding on the stability, conformation, and fluorescence properties of thyroxine-binding globulin.
Topics: Acrylamides; Alpha-Globulins; Drug Stability; Fluorescence Polarization; Guanidine; Guanidines; Humans; Hydrogen-Ion Concentration; Protein Conformation; Spectrometry, Fluorescence; Thyroxine; Thyroxine-Binding Proteins; Tryptophan; Ultracentrifugation | 1982 |
Evidence for intermediates during unfolding and refolding of a two-domain protein, phage T4 lysozyme: equilibrium and kinetic studies.
Topics: Circular Dichroism; Escherichia coli; Guanidine; Guanidines; Kinetics; Mathematics; Models, Molecular; Muramidase; Protein Conformation; Spectrometry, Fluorescence; T-Phages; Tryptophan | 1984 |
The role of the single tryptophan residue in the structure and function of ribonuclease T1.
Topics: Binding Sites; Guanidine; Guanidines; Kinetics; Kynurenine; Oxidation-Reduction; Protein Binding; Ribonuclease T1; Ribonucleases; Spectrometry, Fluorescence; Tryptophan | 1982 |
Dissociation of apolipoprotein AI from apoprotein-lipid complexes and from high-density lipoproteins. A fluorescence study.
Topics: Apolipoprotein A-I; Apolipoproteins; Dimyristoylphosphatidylcholine; Guanidine; Guanidines; Humans; Kinetics; Lipoproteins, LDL; Phosphatidylcholines; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan | 1982 |
Effects of lipid fluidity on quenching characteristics of tryptophan fluorescence in yeast plasma membrane.
Topics: Cell Membrane; Guanidine; Guanidines; Kinetics; Membrane Fluidity; Membrane Proteins; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Temperature; Tryptophan | 1982 |
Investigation of the folding pathway of the TEM-1 beta-lactamase.
Topics: beta-Lactamases; Circular Dichroism; Dose-Response Relationship, Drug; Escherichia coli; Guanidine; Guanidines; Kinetics; Mathematics; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Regression Analysis; Spectrometry, Fluorescence; Time Factors; Tryptophan | 1995 |
Phosphorescence lifetime of tryptophan in proteins.
Topics: Amino Acid Sequence; Cysteine; Glyceraldehyde-3-Phosphate Dehydrogenases; Guanidine; Guanidines; Histidine; Hydrogen-Ion Concentration; Kinetics; L-Lactate Dehydrogenase; Luminescent Measurements; Molecular Sequence Data; Peptides; Protein Denaturation; Proteins; Tryptophan; Tyrosine; Viscosity | 1995 |
Probing intradomain and interdomain conformational changes during equilibrium unfolding of phosphoglycerate kinase: fluorescence and circular dichroism study of tryptophan mutants.
Topics: Circular Dichroism; Fluorescence Polarization; Guanidine; Guanidines; Models, Molecular; Mutagenesis, Site-Directed; Phosphoglycerate Kinase; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Tryptophan; Yeasts | 1995 |
Sequential domain unfolding in phosphoglycerate kinase: fluorescence intensity and anisotropy stopped-flow kinetics of several tryptophan mutants.
Topics: Fluorescence Polarization; Guanidine; Guanidines; Kinetics; Mutagenesis, Site-Directed; Phosphoglycerate Kinase; Protein Denaturation; Protein Folding; Tryptophan; Yeasts | 1995 |
The folding and assembly pathway of tumour necrosis factor TNF alpha, a globular trimeric protein.
Topics: Anilino Naphthalenesulfonates; Cross-Linking Reagents; Glutaral; Guanidine; Guanidines; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan; Tumor Necrosis Factor-alpha | 1995 |
Mutational analysis of the roles in catalysis and substrate recognition of arginines 54 and 305, aspartic acid 309, and tryptophan 317 located at subsites 1 and 2 in glucoamylase from Aspergillus niger.
Topics: Arginine; Aspartic Acid; Aspergillus niger; Base Sequence; Catalysis; DNA Mutational Analysis; DNA Primers; Enzyme Stability; Glucan 1,4-alpha-Glucosidase; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Substrate Specificity; Thermodynamics; Tryptophan | 1995 |
Characterization of two fluorescent tryptophans in recombinant human granulocyte-colony stimulating factor: comparison of native sequence protein and tryptophan-deficient mutants.
Topics: Cloning, Molecular; Granulocyte Colony-Stimulating Factor; Guanidine; Guanidines; Humans; Hydrogen-Ion Concentration; Mutagenesis, Site-Directed; Spectrometry, Fluorescence; Tryptophan | 1993 |
Folding and aggregation of TEM beta-lactamase: analogies with the formation of inclusion bodies in Escherichia coli.
Topics: Anilino Naphthalenesulfonates; beta-Lactamases; Binding Sites; Escherichia coli; Guanidine; Guanidines; Inclusion Bodies; Kinetics; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan | 1994 |
The refolding of hen egg white riboflavin binding protein.
Topics: Animals; Carrier Proteins; Chickens; Dithiothreitol; Edetic Acid; Female; Guanidine; Guanidines; Membrane Transport Proteins; Phosphorylation; Protein Conformation; Protein Denaturation; Protein Folding; Riboflavin; Spectrometry, Fluorescence; Tryptophan; Urea | 1995 |
Guanidine hydrochloride-induced denaturation of the colicin E1 channel peptide: unfolding of local segments using genetically substituted tryptophan residues.
Topics: Circular Dichroism; Colicins; Guanidine; Guanidines; Mutagenesis, Site-Directed; Peptides; Protein Denaturation; Protein Folding; Thiocyanates; Tryptophan | 1995 |
Evidence for residual structures in an unfolded form of yeast phosphoglycerate kinase.
Topics: Guanidine; Guanidines; Mutagenesis, Site-Directed; Phenylalanine; Phosphoglycerate Kinase; Protein Conformation; Protein Folding; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 1995 |
Contribution of individual tryptophan residues to the fluorescence spectrum of native and denatured forms of human carbonic anhydrase II.
Topics: Carbonic Anhydrases; Guanidine; Guanidines; Humans; In Vitro Techniques; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan | 1995 |
Dynamics of parvalbumin studied by fluorescence emission and triplet absorption spectroscopy of tryptophan.
Topics: Animals; Calcium; Fishes; Guanidine; Guanidines; Kinetics; Luminescent Measurements; Oxygen; Parvalbumins; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan | 1995 |
Scrapie amyloid (prion) protein has the conformational characteristics of an aggregated molten globule folding intermediate.
Topics: Anilino Naphthalenesulfonates; Animals; Binding Sites; Circular Dichroism; Cricetinae; Fluorescent Dyes; Guanidine; Guanidines; Hydrochloric Acid; Mesocricetus; Prions; Protein Conformation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Temperature; Tryptophan | 1994 |
Fluorine-19 nuclear magnetic resonance spectroscopic study of fluorophenylalanine- and fluorotryptophan-labeled avian egg white lysozymes.
Topics: Amino Acid Sequence; Animals; Chickens; Female; Fluorine; Guanidine; Guanidines; Magnetic Resonance Spectroscopy; Mass Spectrometry; Muramidase; Mutagenesis, Site-Directed; p-Fluorophenylalanine; Phenylalanine; Protein Conformation; Protein Denaturation; Quail; Recombinant Proteins; Tryptophan | 1994 |
Amino/aromatic interactions in proteins: is the evidence stacked against hydrogen bonding?
Topics: Amino Acid Sequence; Amino Acids; Arginine; Asparagine; Benzene; Calorimetry; Guanidine; Guanidines; Histidine; Hydrogen Bonding; Mathematics; Models, Molecular; Phenylalanine; Protein Conformation; Proteins; Tryptophan; Tyrosine | 1994 |
Characterization of the type I dehydroquinase from Salmonella typhi.
Topics: Circular Dichroism; Diethyl Pyrocarbonate; Escherichia coli; Guanidine; Guanidines; Histidine; Hot Temperature; Hydro-Lyases; Kinetics; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Recombinant Proteins; Salmonella typhi; Spectrometry, Fluorescence; Spectrophotometry; Succinimides; Tryptophan | 1993 |
Detection of intermediate protein conformations by room temperature tryptophan phosphorescence spectroscopy during denaturation of Escherichia coli alkaline phosphatase.
Topics: Alkaline Phosphatase; Bacterial Proteins; Enzyme Stability; Escherichia coli; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan | 1993 |
Tryptophan phosphorescence as a structural probe of mitochondrial F1-ATPase epsilon-subunit.
Topics: Adenosine Triphosphate; Animals; Cattle; Cold Temperature; Guanidine; Guanidines; Kinetics; Luminescent Measurements; Mitochondria, Heart; Nucleotides; Proton-Translocating ATPases; Spectrophotometry; Tryptophan; Urea | 1993 |
A step towards understanding the folding mechanism of horseradish peroxidase. Tryptophan fluorescence and circular dichroism equilibrium studies.
Topics: Calcium; Circular Dichroism; Enzyme Stability; Fluorescence; Guanidine; Guanidines; Horseradish Peroxidase; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Tryptophan | 1993 |
The energetics and cooperativity of protein folding: a simple experimental analysis based upon the solvation of internal residues.
Topics: Algorithms; Amino Acid Sequence; Calorimetry; Geobacillus stearothermophilus; Guanidine; Guanidines; Kinetics; Mathematics; Micrococcal Nuclease; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphoglycerate Kinase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Software; Staphylococcus aureus; Thermodynamics; Tryptophan; Urea | 1993 |
Probing local secondary structure by fluorescence: time-resolved and circular dichroism studies of highly purified neurotoxins.
Topics: Animals; Biophysical Phenomena; Biophysics; Bungarotoxins; Circular Dichroism; Cobra Neurotoxin Proteins; Erabutoxins; Guanidine; Guanidines; In Vitro Techniques; Models, Molecular; Neurotoxins; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 1995 |
How to measure and predict the molar absorption coefficient of a protein.
Topics: 1-Propanol; Chemical Phenomena; Chemistry, Physical; Cystine; Guanidine; Guanidines; Proteins; Solvents; Spectrophotometry, Ultraviolet; Tryptophan; Tyrosine; Urea; Water | 1995 |
Conformational changes in the fibronectin binding MSCRAMMs are induced by ligand binding.
Topics: Adhesins, Bacterial; Amino Acid Sequence; Animals; Bacterial Proteins; Cattle; Circular Dichroism; Fibronectins; Guanidine; Guanidines; Ligands; Molecular Sequence Data; Peptide Fragments; Protein Binding; Protein Conformation; Protein Denaturation; Recombinant Proteins; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Staphylococcus aureus; Streptococcus; Streptococcus pyogenes; Tryptophan | 1996 |
Structure-function relationship of xylanase: fluorimetric analysis of the tryptophan environment.
Topics: Actinomycetales; Binding Sites; Bromosuccinimide; Enzyme Inhibitors; Guanidine; Guanidines; Kinetics; Molecular Structure; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Xylan Endo-1,3-beta-Xylosidase; Xylans; Xylosidases | 1996 |
Unfolding pathway in red kidney bean acid phosphatase is dependent on ligand binding.
Topics: Acid Phosphatase; Adenosine Triphosphate; Circular Dichroism; Fabaceae; Fluorescent Dyes; Guanidine; Guanidines; Kinetics; Ligands; Mercaptoethanol; Phosphates; Plants, Medicinal; Potassium Iodide; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 1996 |
High-pressure denaturation of staphylococcal nuclease proline-to-glycine substitution mutants.
Topics: Enzyme Stability; Escherichia coli; Fluorescence; Glycine; Guanidine; Guanidines; Kinetics; Micrococcal Nuclease; Models, Molecular; Mutagenesis; Point Mutation; Pressure; Proline; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Thermodynamics; Tryptophan | 1996 |
Replacement of Trp28 in Escherichia coli thioredoxin by site-directed mutagenesis affects thermodynamic stability but not function.
Topics: Amino Acid Sequence; Base Sequence; Conserved Sequence; DNA Primers; Drug Stability; Escherichia coli; Guanidine; Guanidines; Kinetics; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Mutagenesis, Site-Directed; Point Mutation; Protein Conformation; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Thioredoxins; Tryptophan | 1996 |
Conformation, stability and interactions of corneal keratan sulfate proteoglycan.
Topics: Animals; Cattle; Cesium; Chondroitin Sulfate Proteoglycans; Circular Dichroism; Collagen; Cornea; Guanidine; Guanidines; Hydrogen-Ion Concentration; Keratan Sulfate; Liposomes; Lumican; Phosphatidylcholines; Protein Conformation; Spectrometry, Fluorescence; Temperature; Tryptophan | 1996 |
Thermodynamics of the unfolding and spectroscopic properties of the V66W mutant of Staphylococcal nuclease and its 1-136 fragment.
Topics: Calorimetry; Circular Dichroism; Escherichia coli; Guanidine; Guanidines; Mathematics; Micrococcal Nuclease; Models, Theoretical; Mutagenesis, Site-Directed; Peptide Fragments; Point Mutation; Protein Conformation; Protein Denaturation; Recombinant Proteins; Spectrometry, Fluorescence; Staphylococcus; Thermodynamics; Tryptophan | 1996 |
Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding.
Topics: Binding Sites; Chromatography, Gel; Circular Dichroism; Fluorescence; Guanidine; Guanidines; Lacticaseibacillus casei; Models, Chemical; Models, Molecular; Molecular Probes; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Thymidylate Synthase; Tryptophan; Urea | 1996 |
Motional dynamics of a buried tryptophan reveals the presence of partially structured forms during denaturation of barstar.
Topics: Bacterial Proteins; Base Sequence; Circular Dichroism; DNA Primers; Enzyme Inhibitors; Escherichia coli; Fluorescence Polarization; Guanidine; Guanidines; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Ribonucleases; Spectrometry, Fluorescence; Tryptophan | 1996 |
Enzymatic and fluorescence studies of four single-tryptophan mutants of rat testis fructose 6-phosphate,2-kinase:fructose 2,6-bisphosphatase.
Topics: Animals; Escherichia coli; Fluorescence Polarization; Guanidine; Guanidines; Male; Multienzyme Complexes; Mutagenesis, Site-Directed; Phosphofructokinase-2; Phosphoric Monoester Hydrolases; Phosphotransferases; Point Mutation; Rats; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Testis; Tryptophan | 1996 |
Conformational changes of alpha-lactalbumin induced by the stepwise reduction of its disulfide bridges: the effect of the disulfide bridges on the structural stability of the protein in sodium dodecyl sulfate solution.
Topics: Anilino Naphthalenesulfonates; Animals; Cattle; Circular Dichroism; Detergents; Disulfides; Fluorescent Dyes; Guanidine; Guanidines; Lactalbumin; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Tryptophan | 1995 |
Role of the intersubunit disulfide bond in the unfolding pathway of dimeric red kidney bean purple acid phosphatase.
Topics: Acid Phosphatase; Anilino Naphthalenesulfonates; Chromatography, Gel; Circular Dichroism; Disulfides; Enzyme Stability; Fabaceae; Glycoproteins; Guanidine; Guanidines; Plants, Medicinal; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 1996 |
Competition between DsbA-mediated oxidation and conformational folding of RTEM1 beta-lactamase.
Topics: beta-Lactamases; Disulfides; Escherichia coli; Guanidine; Guanidines; Isomerases; Kinetics; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Disulfide-Isomerases; Protein Folding; Spectrometry, Fluorescence; Sulfhydryl Compounds; Thermodynamics; Tryptophan | 1996 |
Kinetic and thermodynamic studies of the folding/unfolding of a tryptophan-containing mutant of ribonuclease A.
Topics: Animals; Cattle; Enzyme Stability; Fluorescence; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Mutagenesis, Site-Directed; Mutation; Protein Denaturation; Protein Folding; Ribonuclease, Pancreatic; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan | 1996 |
In vitro renaturation of bovine beta-lactoglobulin A leads to a biologically active but incompletely refolded state.
Topics: Animals; Cattle; Guanidine; Guanidines; Lactoglobulins; Luminescent Measurements; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Vitamin A | 1996 |
Role of non-native aromatic and hydrophobic interactions in the folding of hen egg white lysozyme.
Topics: Animals; Chickens; Egg Proteins; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Muramidase; Mutation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan | 1996 |
Urea-induced equilibrium unfolding of single tryptophan mutants of yeast phosphoglycerate kinase: evidence for a stable intermediate.
Topics: Circular Dichroism; Guanidine; Guanidines; Kinetics; Phosphoglycerate Kinase; Point Mutation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1996 |
Domain behavior during the folding of a thermostable phosphoglycerate kinase.
Topics: Circular Dichroism; Enzyme Stability; Fluorescence; Geobacillus stearothermophilus; Guanidine; Guanidines; Kinetics; Models, Chemical; Models, Molecular; Mutation; Phosphoglycerate Kinase; Polymerase Chain Reaction; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Recombinant Proteins; Thermodynamics; Tryptophan | 1996 |
A 19F-NMR study of the equilibrium unfolding of membrane-associated D-lactate dehydrogenase of Escherichia coli.
Topics: Circular Dichroism; Detergents; Escherichia coli; Fluorine; Guanidine; Guanidines; L-Lactate Dehydrogenase; Lysophosphatidylcholines; Magnetic Resonance Spectroscopy; Membranes; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Solvents; Spectrometry, Fluorescence; Tryptophan | 1996 |
A lysine 73-->histidine variant of yeast iso-1-cytochrome c: evidence for a native-like intermediate in the unfolding pathway and implications for m value effects.
Topics: Circular Dichroism; Cytochrome c Group; Cytochromes c; Fluorescence; Fungal Proteins; Guanidine; Guanidines; Hydrogen-Ion Concentration; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Thermodynamics; Tryptophan; Urea | 1997 |
Stability and folding of a mutant ribose-binding protein of Escherichia coli.
Topics: Carrier Proteins; Circular Dichroism; Crystallography, X-Ray; Escherichia coli; Escherichia coli Proteins; Guanidine; Guanidines; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Periplasmic Binding Proteins; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 1996 |
Submillisecond protein folding kinetics studied by ultrarapid mixing.
Topics: Animals; Cytochrome c Group; Fluorescence; Guanidine; Guanidines; Heme; Horses; Imidazoles; Kinetics; Myocardium; Protein Denaturation; Protein Folding; Tryptophan | 1997 |
Characterization of an unfolding intermediate and kinetic analysis of guanidine hydrochloride-induced denaturation of the colicin E1 channel peptide.
Topics: Anilino Naphthalenesulfonates; Chromatography, Gel; Colicins; Cross-Linking Reagents; Dimerization; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Kinetics; Membrane Proteins; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 1997 |
Rapid formation of a molten globule intermediate in refolding of alpha-lactalbumin.
Topics: Animals; Cattle; Circular Dichroism; Guanidine; Guanidines; Hydrogen Bonding; Kinetics; Lactalbumin; Magnetic Resonance Spectroscopy; Models, Chemical; Molecular Structure; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 1996 |
Glucose modification of human serum albumin: a structural study.
Topics: Amino Acids; Circular Dichroism; Copper; Electrophoresis, Polyacrylamide Gel; Glucose; Glycation End Products, Advanced; Glycosylation; Guanidine; Guanidines; Humans; Pentetic Acid; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Serum Albumin; Spectrometry, Fluorescence; Tryptophan | 1997 |
Denaturation of uridine phosphorylase from Escherichia coli K-12 by guanidine hydrochloride.
Topics: Enzyme Activation; Escherichia coli; Guanidine; Guanidines; Kinetics; Protein Binding; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Ultracentrifugation; Uridine Phosphorylase | 1997 |
Roles of histidine 31 and tryptophan 34 in the structure, self-association, and folding of murine interleukin-6.
Topics: Amino Acid Sequence; Animals; Biological Assay; Circular Dichroism; Guanidine; Guanidines; Histidine; Hydrogen-Ion Concentration; Interleukin-6; Magnetic Resonance Spectroscopy; Mice; Models, Chemical; Models, Molecular; Molecular Sequence Data; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Sequence Homology, Amino Acid; Species Specificity; Structure-Activity Relationship; Titrimetry; Tryptophan | 1997 |
The unusual stability of saporin, a candidate for the synthesis of immunotoxins.
Topics: Circular Dichroism; Drug Stability; Electrophoresis, Polyacrylamide Gel; Endopeptidases; Guanidine; Guanidines; Hydrogen-Ion Concentration; Immunotoxins; Kinetics; N-Glycosyl Hydrolases; Plant Proteins; Protein Denaturation; Protein Folding; Recombinant Fusion Proteins; Ribosome Inactivating Proteins, Type 1; Saporins; Seeds; Spectrometry, Fluorescence; Spectrophotometry; Temperature; Tryptophan | 1997 |
Folding of tryptophan mutants of barstar: evidence for an initial hydrophobic collapse on the folding pathway.
Topics: Bacterial Proteins; Enzyme Inhibitors; Guanidine; Guanidines; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrophotometry; Temperature; Tryptophan | 1997 |
Role of phospholipids in the protein stability of an insect lipoprotein, lipophorin from Rhodnius prolixus.
Topics: Animals; Carrier Proteins; Circular Dichroism; Female; Guanidine; Guanidines; Lipids; Lipoproteins; Phospholipids; Protein Denaturation; Rhodnius; Spectrometry, Fluorescence; Tryptophan | 1997 |
The effect of the metal ion on the folding energetics of azurin: a comparison of the native, zinc and apoprotein.
Topics: Apoproteins; Azurin; Circular Dichroism; Copper; Guanidine; Kinetics; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Zinc | 1997 |
The pH-dependent tertiary structure of a designed helix-loop-helix dimer.
Topics: Amino Acid Sequence; Circular Dichroism; Dimerization; Guanidine; Helix-Loop-Helix Motifs; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Models, Molecular; Models, Theoretical; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Engineering; Protein Structure, Tertiary; Proteins; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1997 |
An early intermediate in the folding reaction of the B1 domain of protein G contains a native-like core.
Topics: Amino Acid Sequence; Antigens, Bacterial; Bacterial Proteins; Binding Sites; Guanidine; Immunoglobulin G; Kinetics; Models, Chemical; Molecular Sequence Data; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Thermodynamics; Tryptophan | 1997 |
Time-resolved protein phosphorescence in the stopped-flow: denaturation of horse liver alcohol dehydrogenase by urea and guanidine hydrochloride.
Topics: Alcohol Dehydrogenase; Animals; Circular Dichroism; Guanidine; Horses; Kinetics; Liver; Luminescent Measurements; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Sodium Chloride; Spectrometry, Fluorescence; Spectrum Analysis; Tryptophan; Urea | 1997 |
Use of Trp mutations to evaluate the conformational behavior and membrane insertion of A and B chains in whole diphtheria toxin.
Topics: Diphtheria Toxin; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Lipid Bilayers; Mutagenesis, Site-Directed; Oligoribonucleotides; Peptide Fragments; Phospholipids; Protein Conformation; Recombinant Proteins; Trypsin; Tryptophan | 1997 |
Analysis of the conformational stability of the active domain of recombinant mouse TIMP-1 by intrinsic fluorescence.
Topics: Acrylamide; Acrylamides; Animals; Binding Sites; Guanidine; Mice; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tissue Inhibitor of Metalloproteinase-1; Tryptophan | 1998 |
The folding kinetics and thermodynamics of the Fyn-SH3 domain.
Topics: Amino Acid Sequence; Escherichia coli; Guanidine; Humans; In Vitro Techniques; Kinetics; Models, Molecular; Molecular Sequence Data; Proline; Protein Denaturation; Protein Folding; Protein-Tyrosine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fyn; Recombinant Fusion Proteins; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; src Homology Domains; Thermodynamics; Tryptophan | 1998 |
Comparison of structural stabilities of prostaglandin H synthase-1 and -2.
Topics: Binding Sites; Circular Dichroism; Enzyme Stability; Guanidine; Humans; Isoenzymes; Prostaglandin-Endoperoxide Synthases; Protein Conformation; Protein Denaturation; Recombinant Proteins; Solvents; Spectrometry, Fluorescence; Tryptophan | 1998 |
Conformation of apolipoprotein AI in reconstituted lipoprotein particles and particle-membrane interaction: effect of cholesterol.
Topics: Antibodies, Monoclonal; Apolipoprotein A-I; Binding, Competitive; Cholesterol; Epitopes; Fluorescence; Guanidine; Lipoproteins, HDL; Liposomes; Particle Size; Permeability; Phosphatidylcholines; Picolinic Acids; Protein Conformation; Protein Denaturation; Terbium; Tryptophan | 1998 |
A stable, molten-globule-like cytochrome c.
Topics: Circular Dichroism; Cytochrome c Group; Escherichia coli; Guanidine; Heme; Oxidation-Reduction; Protein Denaturation; Protein Folding; Recombinant Proteins; Sequence Analysis; Thermus thermophilus; Tryptophan | 1998 |
The origin of the alpha-domain intermediate in the folding of hen lysozyme.
Topics: Animals; Chickens; Circular Dichroism; Fluorescence; Guanidine; Kinetics; Mass Spectrometry; Muramidase; Protein Folding; Protein Structure, Secondary; Protons; Sodium Chloride; Temperature; Tryptophan | 1998 |
Steady state and time-resolved fluorescence study of residual structures in an unfolded form of yeast phosphoglycerate kinase.
Topics: Acrylamide; Acrylamides; Cold Temperature; Diffusion; Guanidine; Kinetics; Mutagenesis, Site-Directed; Phenylalanine; Phosphoglycerate Kinase; Protein Conformation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Solvents; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Viscosity | 1998 |
Characterization of collapsed states in the early stages of the refolding of hen lysozyme.
Topics: Animals; Chickens; Guanidine; Hydrogen-Ion Concentration; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Muramidase; Protein Conformation; Protein Denaturation; Protein Folding; Protons; Spectrometry, Fluorescence; Tryptophan | 1998 |
Incorporation of tryptophan analogues into staphylococcal nuclease: stability toward thermal and guanidine-HCl induced unfolding.
Topics: Amino Acid Substitution; Circular Dichroism; Enzyme Stability; Guanidine; Micrococcal Nuclease; Octanols; Protein Folding; Thermodynamics; Tryptophan; Valine; Water | 1998 |
Multiple kinetic intermediates accumulate during the unfolding of horse cytochrome c in the oxidized state.
Topics: Animals; Circular Dichroism; Cytochrome c Group; Guanidine; Horses; Kinetics; Oxidation-Reduction; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan | 1998 |
Folding kinetics of a fluorescent variant of monomeric lambda repressor.
Topics: Amino Acid Substitution; Bacteriophage lambda; Circular Dichroism; DNA-Binding Proteins; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Repressor Proteins; Spectrometry, Fluorescence; Tryptophan; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 1998 |
Effects of metal binding affinity on the chemical and thermal stability of site-directed mutants of rat oncomodulin.
Topics: Acrylamide; Acrylamides; Animals; Calcium; Calcium-Binding Proteins; Fluorescence; Guanidine; Hydrogen Bonding; Metals; Mutagenesis, Site-Directed; Mutation; Neoplasm Proteins; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Rats; Temperature; Terbium; Thermodynamics; Tryptophan | 1998 |
Role of quaternary structure in muscle creatine kinase stability: tryptophan 210 is important for dimer cohesion.
Topics: Anilino Naphthalenesulfonates; Animals; Chromatography, High Pressure Liquid; Creatine Kinase; Dimerization; Enzyme Stability; Fluorescence; Guanidine; Hydroxymercuribenzoates; Isoenzymes; Mutagenesis, Site-Directed; Myocardium; Protein Conformation; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan | 1998 |
Spectroscopic properties and stability of the neurotoxic complex. Vipoxin and its components.
Topics: Acrylamide; Acrylamides; Drug Stability; Energy Transfer; Enzyme Inhibitors; Guanidine; Kinetics; Models, Molecular; Neurotoxins; Phospholipases A; Phospholipases A2; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Viper Venoms | 1998 |
Purification and characterization of papaya glutamine cyclotransferase, a plant enzyme highly resistant to chemical, acid and thermal denaturation.
Topics: Acrylamide; Aminoacyltransferases; Cysteine Endopeptidases; Glycoproteins; Guanidine; Hydrogen-Ion Concentration; Latex; Plant Proteins; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan | 1998 |
Secondary structure composition and pH-dependent conformational changes of soluble recombinant HLA-DM.
Topics: Circular Dichroism; Guanidine; Histocompatibility Antigens Class II; HLA-D Antigens; Humans; Hydrogen-Ion Concentration; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan | 1998 |
Guanidine-induced denaturation of beta-glycosidase from Sulfolobus solfataricus expressed in Escherichia coli.
Topics: beta-Glucosidase; Chromatography, Gel; Circular Dichroism; Escherichia coli; Guanidine; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Sulfolobus; Temperature; Tryptophan | 1998 |
Thermodynamic and kinetic characterization of calf lens gammaF-crystallin.
Topics: Animals; Cattle; Circular Dichroism; Crystallins; Guanidine; Kinetics; Lens, Crystalline; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1998 |
Mimosa pudica apyrase requires polysaccharide and Ca2+ for the activity.
Topics: Amylases; Apoenzymes; Apyrase; Arabinose; Calcium; Calcium Channel Blockers; Calcium-Transporting ATPases; Chelating Agents; Circular Dichroism; Galactans; Galactose; Guanidine; Isoenzymes; Plant Leaves; Polysaccharides; Protein Structure, Secondary; Trifluoperazine; Tryptophan; Verapamil; Xylan Endo-1,3-beta-Xylosidase; Xylosidases | 1998 |
Evidence for kinetic intermediate states during the refolding of GdnHCl-denatured MM-creatine kinase. Characterization of a trapped monomeric species.
Topics: Anilino Naphthalenesulfonates; Animals; Chemical Phenomena; Chemistry, Physical; Chromatography, Gel; Creatine Kinase; Cysteine; Enzyme Activation; Guanidine; Isoenzymes; Kinetics; Models, Chemical; Protein Binding; Protein Denaturation; Protein Folding; Rabbits; Spectrometry, Fluorescence; Titrimetry; Tryptophan; Tyrosine | 1998 |
Apohorseradish peroxidase unfolding and refolding: intrinsic tryptophan fluorescence studies.
Topics: Apoenzymes; Biophysical Phenomena; Biophysics; Fluorescence Polarization; Guanidine; Horseradish Peroxidase; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 1999 |
Unfolding of Plasmodium falciparum triosephosphate isomerase in urea and guanidinium chloride: evidence for a novel disulfide exchange reaction in a covalently cross-linked mutant.
Topics: Animals; Chromatography, Gel; Circular Dichroism; Cross-Linking Reagents; Cysteine; Disulfides; Guanidine; Models, Molecular; Mutagenesis, Site-Directed; Plasmodium falciparum; Protein Conformation; Protein Denaturation; Recombinant Proteins; Spectrometry, Fluorescence; Triose-Phosphate Isomerase; Tryptophan; Tyrosine; Urea | 1999 |
Dynamic equilibrium unfolding pathway of human tumor necrosis factor-alpha induced by guanidine hydrochloride.
Topics: Anisotropy; Buffers; Guanidine; Humans; Potassium Iodide; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Time Factors; Tryptophan; Tumor Necrosis Factor-alpha | 1999 |
Autophosphorylation dependent destabilization of the insulin receptor kinase domain: tryptophan-1175 reports changes in the catalytic cleft.
Topics: Amino Acid Substitution; Catalytic Domain; Enzyme Stability; Fluorescence Polarization; Guanidine; Humans; Models, Molecular; Peptide Fragments; Phenylalanine; Phosphorylation; Protein Denaturation; Protein Structure, Secondary; Receptor, Insulin; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1999 |
Structural and dynamic aspects of beta-glycosidase from mesophilic and thermophilic bacteria by multitryptophanyl emission decay studies.
Topics: beta-Glucosidase; Cyclohexanols; Enzyme Inhibitors; Escherichia coli; Guanidine; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Sulfolobus; Tryptophan | 1999 |
Colicin E1 forms a dimer after urea-induced unfolding.
Topics: Amino Acid Sequence; Amino Acid Substitution; Chromatography, High Pressure Liquid; Circular Dichroism; Colicins; Dimerization; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Models, Molecular; Molecular Sequence Data; Naphthalenesulfonates; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
Ligand exchange during unfolding of cytochrome c.
Topics: Animals; Circular Dichroism; Cytochrome c Group; Guanidine; Heme; Horses; Kinetics; Ligands; Myocardium; Protein Denaturation; Protein Folding; Scattering, Radiation; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1999 |
Folding dynamics of the B1 domain of protein G explored by ultrarapid mixing.
Topics: Fluorescence; Guanidine; Kinetics; Models, Chemical; Nerve Tissue Proteins; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Solvents; Thermodynamics; Time Factors; Tryptophan | 1999 |
The molten globule state of a chimera of human alpha-lactalbumin and equine lysozyme.
Topics: Acrylamide; Amino Acid Sequence; Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Enzyme Stability; Fluorescent Dyes; Guanidine; Horses; Humans; Hydrogen-Ion Concentration; Lactalbumin; Molecular Sequence Data; Muramidase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Fusion Proteins; Solvents; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1999 |
Effect of self-association on the structural organization of partially folded proteins: inactivated actin.
Topics: Acrylamide; Actins; Anilino Naphthalenesulfonates; Animals; Guanidine; Protein Conformation; Protein Folding; Protein Multimerization; Protein Unfolding; Rabbits; Solvents; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan | 1999 |
The tetrameric protein transthyretin dissociates to a non-native monomer in solution. A novel model for amyloidogenesis.
Topics: Amyloid; Amyloid Neuropathies; Anilino Naphthalenesulfonates; Chromatography, Gel; Guanidine; Humans; Iodides; Models, Molecular; Prealbumin; Protein Conformation; Recombinant Proteins; Solubility; Spectrometry, Fluorescence; Tryptophan | 1999 |
Analysis of folding and unfolding reactions of cytochrome b5.
Topics: Animals; Cattle; Cytochromes b5; Guanidine; Holoenzymes; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan | 1999 |
Fluorescence and circular dichroism spectroscopic studies on bovine lactoperoxidase.
Topics: Acrylamide; Animals; Cattle; Cesium; Circular Dichroism; Fluorescent Dyes; Guanidine; Iodine; Lactoperoxidase; Milk; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
Structural and conformational stability of horseradish peroxidase: effect of temperature and pH.
Topics: Calcium; Circular Dichroism; Enzyme Stability; Guanidine; Heme; Horseradish Peroxidase; Hydrogen-Ion Concentration; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Temperature; Tryptophan | 2000 |
Acrylamide quenching of apo- and holo-alpha-lactalbumin in guanidine hydrochloride.
Topics: Acrylamide; Animals; Apoproteins; Cattle; Guanidine; Kinetics; Lactalbumin; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2000 |
Two-state expansion and collapse of a polypeptide.
Topics: Animals; Computer Simulation; Cytochrome c Group; Diffusion; Guanidine; Heme; Horses; Kinetics; Lasers; Models, Chemical; Peptides; Protein Conformation; Protein Denaturation; Protein Folding; Solvents; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Viscosity | 2000 |
Heme orientation affects holo-myoglobin folding and unfolding kinetics.
Topics: Animals; Apoproteins; Circular Dichroism; Dose-Response Relationship, Drug; Fluorescence; Guanidine; Heme; Horses; Kinetics; Metmyoglobin; Myoglobin; Protein Denaturation; Protein Folding; Protein Renaturation; Rotation; Thermodynamics; Tryptophan; Whales | 2000 |
Steady state and time resolved effects of guanidine hydrochloride on the structure of Humicola lanuginosa lipase revealed by fluorescence spectroscopy.
Topics: Amino Acid Substitution; Ascomycota; Binding Sites; Guanidine; Lipase; Mutation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2000 |
Alanine mutagenesis of high-mobility-group-protein-1 box B (HMG1-B).
Topics: Alanine; Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution; Animals; Carrier Proteins; Circular Dichroism; DNA; DNA-Binding Proteins; Fluorescence; Guanidine; High Mobility Group Proteins; HMGB1 Protein; Models, Molecular; Molecular Sequence Data; Mutation; Phenylalanine; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Rats; Temperature; Thermodynamics; Tryptophan | 2000 |
Evidence for tryptophan in proximity to histidine and cysteine as essential to the active site of an alkaline protease.
Topics: Affinity Labels; Binding Sites; Chromatography, DEAE-Cellulose; Conidiobolus; Cysteine; Endopeptidases; Energy Transfer; Fluoresceins; Guanidine; Histidine; Kinetics; Spectrometry, Fluorescence; Tryptophan; Ultrafiltration | 2000 |
Lipid binding of the exchangeable apolipoprotein apolipophorin III induces major changes in fluorescence properties of tryptophans 115 and 130.
Topics: Acrylamide; Animals; Apolipoproteins; Circular Dichroism; Dimyristoylphosphatidylcholine; Grasshoppers; Guanidine; Insect Proteins; Lipids; Mutation; Nephelometry and Turbidimetry; Protein Binding; Protein Structure, Secondary; Spectrometry, Fluorescence; Trypsin; Tryptophan | 2000 |
Fluorescence energy transfer indicates similar transient and equilibrium intermediates in staphylococcal nuclease folding.
Topics: Amino Acid Substitution; Anilino Naphthalenesulfonates; Anions; Circular Dichroism; Cysteine; Energy Transfer; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Models, Molecular; Pliability; Proline; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Thermodynamics; Titrimetry; Tryptophan; Urea | 2000 |
Effects of side-chain characteristics on stability and oligomerization state of a de novo-designed model coiled-coil: 20 amino acid substitutions in position "d".
Topics: Algorithms; Amino Acid Sequence; Amino Acid Substitution; Amino Acids; Aspartic Acid; Chromatography, High Pressure Liquid; Circular Dichroism; Disulfides; Guanidine; Methionine; Models, Molecular; Molecular Sequence Data; Protein Denaturation; Protein Structure, Quaternary; Protein Structure, Secondary; Proteins; Static Electricity; Structure-Activity Relationship; Thermodynamics; Tryptophan; Ultracentrifugation | 2000 |
Tryptophan 140 is important, but serine 141 Is essential for the formation of the integrated conformation of staphylococcal nuclease.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescent Dyes; Guanidine; Micrococcal Nuclease; Peptide Fragments; Protein Folding; Recombinant Proteins; Serine; Spectrometry, Fluorescence; Tryptophan | 2000 |
States of tryptophyl residues and stability of recombinant human matrix metalloproteinase 7 (matrilysin) as examined by fluorescence.
Topics: Collagenases; Enzyme Stability; Escherichia coli; Fluorescent Dyes; Guanidine; Humans; Matrix Metalloproteinase 7; Protein Denaturation; Recombinant Proteins; Sodium Chloride; Spectrometry, Fluorescence; Temperature; Tryptophan | 2000 |
Nanosecond dynamics of the single tryptophan reveals multi-state equilibrium unfolding of protein GB1.
Topics: Antigens, Bacterial; Bacterial Proteins; Circular Dichroism; Fluorescence Polarization; Guanidine; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Receptors, IgG; Spectrometry, Fluorescence; Streptococcus; Tryptophan | 2000 |
Anion-induced folding of rabbit muscle pyruvate kinase: existence of multiple intermediate conformations at low pH.
Topics: Anilino Naphthalenesulfonates; Animals; Guanidine; Hydrogen-Ion Concentration; In Vitro Techniques; Muscles; Protein Conformation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Pyruvate Kinase; Rabbits; Solvents; Tryptophan | 2000 |
Characterization of acid-induced unfolding intermediates of glucose/xylose isomerase.
Topics: Aldose-Ketose Isomerases; Anilino Naphthalenesulfonates; Circular Dichroism; Dimerization; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Light; Osmolar Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Scattering, Radiation; Spectrophotometry, Ultraviolet; Streptomyces; Tryptophan | 2000 |
Distributions of intramolecular distances in the reduced and denatured states of bovine pancreatic ribonuclease A. Folding initiation structures in the C-terminal portions of the reduced protein.
Topics: Animals; Cattle; Crystallography, X-Ray; Dithiothreitol; Energy Transfer; Fluorescence Polarization; Fluorescent Dyes; Guanidine; Mutagenesis, Site-Directed; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Reducing Agents; Ribonuclease, Pancreatic; Spectrometry, Fluorescence; Tryptophan | 2001 |
Native fluorescence and mag-indo-1-protein interaction as tools for probing unfolding and refolding sequences of the bovine serum albumin subdomain in the presence of guanidine hydrochloride.
Topics: Animals; Calcium; Cattle; Fluorescent Dyes; Guanidine; Humans; Indoles; Models, Molecular; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Serum Albumin, Bovine; Spectrometry, Fluorescence; Tryptophan | 2000 |
Probing conformational changes in the estrogen receptor: evidence for a partially unfolded intermediate facilitating ligand binding and release.
Topics: Anilino Naphthalenesulfonates; Binding Sites; Estrogen Receptor alpha; Fluorescence; Fluorescent Dyes; Guanidine; Humans; Protein Conformation; Protein Denaturation; Protein Folding; Receptors, Estrogen; Spectrometry, Fluorescence; Tryptophan | 2001 |
Cofactor-induced refolding: refolding of molten globule carbonic anhydrase induced by Zn(II) and Co(II).
Topics: Anilino Naphthalenesulfonates; Animals; Apoenzymes; Binding Sites; Carbonic Anhydrases; Cattle; Circular Dichroism; Cobalt; Fluorescent Dyes; Guanidine; Holoenzymes; Kinetics; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 2001 |
Association and activation of fructose 1,6-bisphosphase during unfolding and refolding: spectroscopic and enzymatic studies.
Topics: Amino Acid Substitution; Circular Dichroism; Enzyme Activation; Fluorescence; Fructose-Bisphosphatase; Guanidine; Magnesium; Mutation; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Urea | 2001 |
Differences in the pathways for unfolding and hydrogen exchange among mutants of Escherichia coli alkaline phosphatase.
Topics: Alkaline Phosphatase; Bacterial Proteins; Deuterium; Escherichia coli; Guanidine; Hydrogen; Hydrogen Bonding; Kinetics; Luminescent Measurements; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Fusion Proteins; Thermodynamics; Tryptophan | 2001 |
Understanding the role of internal lysine residues of serum albumins in conformational stability and bilirubin binding.
Topics: Acetylation; Animals; Bilirubin; Chloroform; Chromatography, Gel; Circular Dichroism; Guanidine; Humans; Lysine; Molecular Weight; Protein Binding; Protein Conformation; Rabbits; Ruminants; Serum Albumin; Spectrometry, Fluorescence; Succinates; Tryptophan; Ultraviolet Rays | 2001 |
Snapshots of protein folding. A study on the multiple transition state pathway of cytochrome c(551) from Pseudomonas aeruginosa.
Topics: Acids; Bacterial Proteins; Cytochrome c Group; Fluorescence; Glutamic Acid; Guanidine; Hydrogen Bonding; Kinetics; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Static Electricity; Thermodynamics; Tryptophan; Urea | 2001 |
Tryptophan phosphorescence as a monitor of protein conformation in molecular films.
Topics: Alcohol Dehydrogenase; Animals; Biosensing Techniques; Enzymes, Immobilized; Glutamate Dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenases; Guanidine; In Vitro Techniques; Luminescent Measurements; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan | 2000 |
Cofactor and tryptophan accessibility and unfolding of brain glutamate decarboxylase.
Topics: Acrylamide; Animals; Anions; Binding Sites; Brain; Cell Line; Cesium; Circular Dichroism; Dimerization; Dose-Response Relationship, Drug; Glutamate Decarboxylase; Guanidine; Insecta; Iodine; Ions; Kinetics; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Rats; Spectrometry, Fluorescence; Tryptophan | 2001 |
Characterization of the folding and unfolding reactions of a small beta-barrel protein of novel topology, the MTCP1 oncogene product P13.
Topics: Circular Dichroism; Fluorescence; Guanidine; Magnetic Resonance Spectroscopy; Protein Conformation; Protein Denaturation; Protein Folding; Proto-Oncogene Proteins; Tryptophan | 2001 |
An engineered transthyretin monomer that is nonamyloidogenic, unless it is partially denatured.
Topics: Amino Acid Substitution; Amyloid; Binding Sites; Crystallography, X-Ray; Dimerization; Drug Stability; Fluorescent Dyes; Guanidine; Humans; Hydrogen-Ion Concentration; Macromolecular Substances; Models, Molecular; Mutagenesis, Site-Directed; Nuclear Magnetic Resonance, Biomolecular; Prealbumin; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Ultracentrifugation; Urea | 2001 |
p53 unfolding detected by CD but not by tryptophan fluorescence.
Topics: Circular Dichroism; Guanidine; Humans; Potassium Iodide; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Tumor Suppressor Protein p53; Urea | 2001 |
Tryptophan mutants of intestinal fatty acid-binding protein: ultraviolet absorption and circular dichroism studies.
Topics: Anilino Naphthalenesulfonates; Carrier Proteins; Circular Dichroism; Dose-Response Relationship, Drug; Fatty Acid-Binding Proteins; Fluorescent Dyes; Guanidine; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Neoplasm Proteins; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Receptors, Retinoic Acid; Spectrophotometry; Spectrophotometry, Ultraviolet; Tryptophan; Ultraviolet Rays | 2001 |
Protein folding pathways of adenylate kinase from E. coli: hydrostatic pressure and stopped-flow studies.
Topics: Adenylate Kinase; Binding Sites; Circular Dichroism; Escherichia coli; Guanidine; Hydrostatic Pressure; Models, Molecular; Mutagenesis, Site-Directed; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrophotometry; Tryptophan | 2001 |
Creating hetero-11-mers composed of wild-type and mutant subunits to study RNA binding to TRAP.
Topics: Bacillus subtilis; Bacterial Proteins; Circular Dichroism; Dose-Response Relationship, Drug; Escherichia coli; Guanidine; Kinetics; Models, Chemical; Mutation; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; RNA; RNA-Binding Proteins; Time Factors; Transcription Factors; Tryptophan; Ultracentrifugation | 2002 |
Unfolding of triosephosphate isomerase from Trypanosoma brucei: identification of intermediates and insight into the denaturation pathway using tryptophan mutants.
Topics: Animals; Catalysis; Circular Dichroism; Fluorescence; Guanidine; Mutagenesis, Site-Directed; Protein Denaturation; Triose-Phosphate Isomerase; Trypanosoma brucei brucei; Tryptophan | 2002 |
Probing the role of the F-helix in serpin stability through a single tryptophan substitution.
Topics: Alanine; alpha 1-Antitrypsin; Amino Acid Sequence; Amino Acid Substitution; Drug Stability; Guanidine; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Serpins; Spectrometry, Fluorescence; Tryptophan | 2002 |
A pH dependence study on the unfolding and refolding of apoazurin: comparison with Zn(II) azurin.
Topics: Apoproteins; Azurin; Chelating Agents; Circular Dichroism; Crystallography, X-Ray; Dose-Response Relationship, Drug; Edetic Acid; Guanidine; Hydrogen-Ion Concentration; Kinetics; Ligands; Models, Chemical; Protein Binding; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Spectrophotometry; Thermodynamics; Time Factors; Tryptophan; Zinc | 2002 |
Guanidine hydrochloride induced equilibrium unfolding studies of colicin B and its channel-forming fragment.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Colicins; Guanidine; Hydrogen-Ion Concentration; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2002 |
Hydration at the surface of the protein Monellin: dynamics with femtosecond resolution.
Topics: Guanidine; Kinetics; Models, Molecular; Plant Proteins; Protein Conformation; Protein Folding; Time Factors; Tryptophan | 2002 |
Cooperative interactions and a non-native buried Trp in the unfolded state of an SH3 domain.
Topics: Allosteric Regulation; Allosteric Site; Amino Acid Sequence; Circular Dichroism; Drosophila Proteins; Guanidine; Hydrogen; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; src Homology Domains; Temperature; Thermodynamics; Tryptophan | 2002 |
Purification and characterization of two polymorphic variants of short chain acyl-CoA dehydrogenase reveal reduction of catalytic activity and stability of the Gly185Ser enzyme.
Topics: Acyl-CoA Dehydrogenase; Acyl-CoA Dehydrogenases; Amino Acid Substitution; Arginine; Catalysis; Chaperonin 10; Chaperonin 60; Circular Dichroism; Enzyme Stability; Escherichia coli; Fatty Acids, Volatile; Genetic Variation; Glycine; Guanidine; Hot Temperature; Humans; Oxidation-Reduction; Polymorphism, Genetic; Protein Denaturation; Protein Folding; Recombinant Proteins; Serine; Spectrophotometry; Tryptophan | 2002 |
Role of entropy in protein thermostability: folding kinetics of a hyperthermophilic cold shock protein at high temperatures using 19F NMR.
Topics: Bacterial Proteins; Calorimetry, Differential Scanning; Carrier Proteins; Entropy; Fluorine; Guanidine; Heat-Shock Proteins; Hot Temperature; Kinetics; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Spectrophotometry; Thermotoga maritima; Tryptophan | 2002 |
Physicochemical characterisation of the two active site mutants Trp(52)-->Phe and Asp(55)-->Val of glucoamylase from Aspergillus niger.
Topics: Amino Acid Substitution; Aspartic Acid; Aspergillus niger; Binding Sites; Calorimetry; Glucan 1,4-alpha-Glucosidase; Glucans; Guanidine; Kinetics; Maltose; Mutation; Phenylalanine; Protein Conformation; Protein Denaturation; Sequence Deletion; Substrate Specificity; Tryptophan; Valine | 2002 |
Kinetic analysis of R67 dihydrofolate reductase folding: from the unfolded monomer to the native tetramer.
Topics: Circular Dichroism; Dimerization; Escherichia coli Proteins; Fluorescence Resonance Energy Transfer; Guanidine; Kinetics; Models, Chemical; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; R Factors; Spectrometry, Fluorescence; Tetrahydrofolate Dehydrogenase; Tryptophan | 2002 |
Interleukin-1 beta folding between pH 5 and 7: experimental evidence for three-state folding behavior and robust transition state positions late in folding.
Topics: Guanidine; Humans; Hydrogen-Ion Concentration; Interleukin-1; Kinetics; Models, Chemical; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Titrimetry; Tryptophan | 2002 |
How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment.
Topics: Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Dialysis; Disulfides; Dose-Response Relationship, Drug; Escherichia coli; Fluorescent Dyes; Guanidine; Immunoglobulin Fragments; Immunoglobulin Variable Region; Immunoglobulins; Mass Spectrometry; Mice; Models, Chemical; Protein Conformation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Tryptophan | 2003 |
Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants.
Topics: Animals; Circular Dichroism; Fluorescence; Glycolates; Guanidine; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Plasmodium falciparum; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Triose-Phosphate Isomerase; Tryptophan; Urea | 2003 |
Effect of pH on the iso-1-cytochrome c denatured state: changing constraints due to heme ligation.
Topics: Acetylation; Amino Acid Substitution; Binding, Competitive; Cytochrome c Group; Cytochromes c; Genetic Variation; Guanidine; Heme; Histidine; Hydrogen-Ion Concentration; Ligands; Lysine; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Structure, Tertiary; Saccharomyces cerevisiae Proteins; Spectrometry, Fluorescence; Tryptophan | 2003 |
Observed hysteresis of virus capsid disassembly is implicit in kinetic models of assembly.
Topics: Capsid; Dimerization; Dose-Response Relationship, Drug; Escherichia coli; Guanidine; Hepatitis B Core Antigens; Hepatitis B virus; Kinetics; Light; Scattering, Radiation; Thermodynamics; Time Factors; Tryptophan; Urea; Viral Core Proteins; Virion; Virus Assembly | 2003 |
Steady-state tryptophan fluorescence spectroscopy study to probe tertiary structure of proteins in solid powders.
Topics: Chemical Phenomena; Chemical Precipitation; Chemistry, Physical; Crystallization; Guanidine; Interferon alpha-2; Interferon-alpha; Lactoglobulins; Mannitol; Polyethylene Glycols; Powders; Protein Denaturation; Protein Structure, Tertiary; Recombinant Proteins; Spectrometry, Fluorescence; Trehalose; Tryptophan | 2003 |
Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolA.
Topics: Amino Acid Sequence; Anisotropy; Circular Dichroism; Colicins; Cysteine; Escherichia coli; Escherichia coli Proteins; Fluorescence Resonance Energy Transfer; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Surface Plasmon Resonance; Time Factors; Tryptophan; Water | 2003 |
Characterization of single-tryptophan mutants of histidine-containing phosphocarrier protein: evidence for local rearrangements during folding from high concentrations of denaturant.
Topics: Amino Acid Substitution; Bacterial Proteins; Binding Sites; Fluorescent Dyes; Guanidine; Histidine; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Phosphoenolpyruvate Sugar Phosphotransferase System; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2003 |
Equilibrium unfolding of dimeric human prostatic acid phosphatase involves an inactive monomeric intermediate.
Topics: Acid Phosphatase; Catalysis; Catalytic Domain; Chromatography; Chromatography, High Pressure Liquid; Cysteine; Dimerization; Dose-Response Relationship, Drug; Guanidine; Humans; Kinetics; Male; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Protein Tyrosine Phosphatases; Spectrometry, Fluorescence; Thermodynamics; Time Factors; Tryptophan | 2003 |
Chain length dependence of apomyoglobin folding: structural evolution from misfolded sheets to native helices.
Topics: Animals; Apoproteins; Circular Dichroism; Guanidine; Kinetics; Models, Molecular; Myoglobin; Protein Biosynthesis; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Solvents; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Structure-Activity Relationship; Thiazoles; Tryptophan; Whales | 2003 |
Investigating the role of metal ions in the catalytic mechanism of the yeast RNA triphosphatase.
Topics: Acid Anhydride Hydrolases; Adenosine Triphosphate; Binding Sites; Catalysis; Catalytic Domain; Circular Dichroism; Cobalt; Dose-Response Relationship, Drug; Guanidine; Hydrogen-Ion Concentration; Ions; Kinetics; Ligands; Magnesium; Manganese; Phosphoric Monoester Hydrolases; Plasmids; Protein Binding; Protein Denaturation; Protein Folding; RNA; RNA, Messenger; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Ultraviolet Rays | 2003 |
Guanidinium receptors as enantioselective amino acid membrane carriers.
Topics: Amino Acid Transport Systems; Amino Acids; Biomimetic Materials; Ethers, Cyclic; Guanidine; Lasalocid; Membranes, Artificial; Models, Molecular; Stereoisomerism; Substrate Specificity; Thermodynamics; Tryptophan | 2003 |
Unfolding of Vibrio cholerae hemolysin induces oligomerization of the toxin monomer.
Topics: Cell Membrane; Cholesterol; Diffusion; Electrophoresis, Polyacrylamide Gel; Guanidine; Hemolysin Proteins; Lipid Metabolism; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Sphingolipids; Thermodynamics; Toxins, Biological; Tryptophan; Urea; Vibrio cholerae | 2003 |
Highly nonexponential kinetics in the early-phase refolding of proteins at low temperatures.
Topics: Animals; Chickens; Cold Temperature; Cytochrome c Group; Egg Proteins; Guanidine; Horses; Hydrogen-Ion Concentration; Kinetics; Muramidase; Myocardium; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Spectrum Analysis; Tryptophan | 2003 |
Kinetics of intramolecular contact formation in a denatured protein.
Topics: Bacterial Proteins; Guanidine; Heat-Shock Proteins; Models, Molecular; Protein Denaturation; Protein Structure, Secondary; Thermotoga maritima; Tryptophan | 2003 |
STRUCTURAL TRANSITIONS OF LYSOZYME.
Topics: Chemistry Techniques, Analytical; Fluorescence; Guanidine; Guanidines; Hydrogen-Ion Concentration; Muramidase; Research; Spectrum Analysis; Temperature; Tryptophan; Urea | 1964 |
[PAPER CHROMATOGRAPHY OF SEVERAL BIOLOGICALLY IMPORTANT GUANIDINE COMPOUNDS].
Topics: Arginine; Canavanine; Chromatography; Chromatography, Paper; Creatine; Creatinine; Cystine; Guanidine; Guanidines; Isoleucine; Methionine; Research; Serine; Streptomycin; Tryptophan; Valine | 1964 |
Study on Escherichia coli alkaline phosphatase conformation by phosphorimetry in the presence of denaturant.
Topics: Alkaline Phosphatase; Calorimetry; Escherichia coli; Guanidine; Luminescence; Protein Conformation; Protein Denaturation; Thermodynamics; Tryptophan | 2003 |
Induction of 'molten globule' like state in acid-denatured state of unmodified preparation of stem bromelain: implications of disulfides in protein folding.
Topics: Acids; Anilino Naphthalenesulfonates; Bromelains; Circular Dichroism; Disulfides; Fluorescence; Fluorescent Dyes; Guanidine; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Trifluoroethanol; Tryptophan | 2003 |
Monitoring of actin unfolding by room temperature tryptophan phosphorescence.
Topics: Actins; Animals; Guanidine; Kinetics; Luminescent Measurements; Muscle, Skeletal; Protein Denaturation; Protein Folding; Rabbits; Temperature; Tryptophan | 2003 |
Stability and folding kinetics of a ubiquitin mutant with a strong propensity for nonnative beta-hairpin conformation in the unfolded state.
Topics: Amino Acid Sequence; Amino Acid Substitution; Fluorocarbons; Guanidine; Kinetics; Methanol; Models, Molecular; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Sequence Alignment; Spectrometry, Fluorescence; Tryptophan; Ubiquitin | 2003 |
Increase in molecular rigidity of the protein conformation of brain Na+-K+-ATPase by modification with 4-hydroxy-2-nonenal.
Topics: Acrylamide; Aldehydes; Animals; Anisotropy; Brain; Dose-Response Relationship, Drug; Ethylmaleimide; Fluorescence; Guanidine; Lipid Peroxidation; Molecular Conformation; Protein Conformation; Sodium-Potassium-Exchanging ATPase; Sulfhydryl Compounds; Swine; Tryptophan | 2003 |
Tubulin equilibrium unfolding followed by time-resolved fluorescence and fluorescence correlation spectroscopy.
Topics: Animals; Anisotropy; Brain; Chickens; Circular Dichroism; Dimerization; Dose-Response Relationship, Drug; Fluorescence; Fluorescence Polarization; Fluorescent Dyes; Guanidine; Hydrazines; Kinetics; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Rotation; Specimen Handling; Spectrometry, Fluorescence; Tryptophan; Tubulin | 2004 |
Rapid collapse precedes the fast two-state folding of the cold shock protein.
Topics: Amino Acid Substitution; Anilino Naphthalenesulfonates; Bacterial Proteins; Energy Transfer; Guanidine; Heat-Shock Proteins; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Thermodynamics; Tryptophan | 2004 |
Conformational change of the dimeric DsbC molecule induced by GdnHCl. A study by intrinsic fluorescence.
Topics: Dimerization; Escherichia coli Proteins; Guanidine; Kinetics; Models, Chemical; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Disulfide-Isomerases; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 2004 |
Probing folding and fluorescence quenching in human gammaD crystallin Greek key domains using triple tryptophan mutant proteins.
Topics: Amino Acid Substitution; gamma-Crystallins; Guanidine; Humans; Kinetics; Point Mutation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2004 |
X-ray and thermodynamic studies of staphylococcal nuclease variants I92E and I92K: insights into polarity of the protein interior.
Topics: Crystallography, X-Ray; Fluorescence; Glutamic Acid; Guanidine; Hydrogen Bonding; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Denaturation; Thermodynamics; Tryptophan | 2004 |
Contribution of the two conserved tryptophan residues to the catalytic and structural properties of Proteus mirabilis glutathione S-transferase B1-1.
Topics: Amino Acid Substitution; Anti-Bacterial Agents; Catalysis; Conserved Sequence; Enzyme Stability; Glutathione Transferase; Guanidine; Kinetics; Models, Molecular; Protein Binding; Protein Conformation; Protein Denaturation; Protein Renaturation; Proteus mirabilis; Spectrum Analysis; Static Electricity; Structure-Activity Relationship; Temperature; Tryptophan | 2005 |
Conformational changes in azurin from Pseudomona aeruginosa induced through chemical and physical protocols.
Topics: Acrylamide; Azurin; Biophysical Phenomena; Biophysics; Copper; Gadolinium; Guanidine; Hot Temperature; Ligands; Phase Transition; Protein Conformation; Protein Folding; Pseudomonas aeruginosa; Spectrometry, Fluorescence; Temperature; Thermodynamics; Time Factors; Tryptophan | 2004 |
Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride.
Topics: Archaeal Proteins; Carrier Proteins; Crystallography, X-Ray; Guanidine; Hydrogen Bonding; Ions; Models, Molecular; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Secondary; Pyrococcus horikoshii; Spectrophotometry; Temperature; Tryptophan | 2004 |
The tryptophane residues of dimeric arginine kinase: roles of Trp-208 and Trp-218 in active site and conformation stability.
Topics: Animals; Arginine Kinase; Binding Sites; Dimerization; Enzyme Stability; Guanidine; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Spectrometry, Fluorescence; Stichopus; Tryptophan | 2004 |
Identification of residual structure within denatured antichymotrypsin: implications for serpin folding and misfolding.
Topics: alpha 1-Antichymotrypsin; Guanidine; Humans; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteins; Serpins; Spectrometry, Fluorescence; Thermodynamics; Time Factors; Tryptophan | 2004 |
Context-dependent effects of proline residues on the stability and folding pathway of ubiquitin.
Topics: Amino Acid Substitution; Escherichia coli; Guanidine; Humans; Kinetics; Models, Molecular; Proline; Protein Denaturation; Protein Folding; Protein Renaturation; Recombinant Proteins; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Ubiquitin | 2004 |
Dissecting contributions to the denaturant sensitivities of proteins.
Topics: Amino Acid Sequence; Circular Dichroism; Guanidine; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Solutions; Thermodynamics; Tryptophan; Urea | 2005 |
Effects of heme on the structure of the denatured state and folding kinetics of cytochrome b562.
Topics: Amino Acid Sequence; Apoproteins; Circular Dichroism; Cytochrome b Group; Escherichia coli; Escherichia coli Proteins; Guanidine; Heme; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Molecular Sequence Data; Mutation; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Thermodynamics; Tryptophan; Urea | 2005 |
Folding of the SARS coronavirus spike glycoprotein immunological fragment (SARS_S1b): thermodynamic and kinetic investigation correlating with three-dimensional structural modeling.
Topics: Amino Acid Sequence; Circular Dichroism; Computer Simulation; Guanidine; Hot Temperature; Kinetics; Membrane Glycoproteins; Models, Chemical; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Severe acute respiratory syndrome-related coronavirus; Spectrometry, Fluorescence; Spike Glycoprotein, Coronavirus; Structural Homology, Protein; Thermodynamics; Tryptophan; Viral Envelope Proteins | 2005 |
Equilibrium unfolding of neuronal calcium sensor-1: N-terminal myristoylation influences unfolding and reduces protein stiffening in the presence of calcium.
Topics: Amino Acid Substitution; Animals; Calcium; Calcium-Binding Proteins; Guanidine; Mutation; Myristic Acid; Neuronal Calcium-Sensor Proteins; Neuropeptides; Protein Denaturation; Rats; Spectrometry, Fluorescence; Trypsin; Tryptophan | 2005 |
Conformational study of spectrin in presence of submolar concentrations of denaturants.
Topics: 2-Naphthylamine; Animals; Binding Sites; Dimerization; Fluorescence Polarization; Fluorescent Dyes; Guanidine; In Vitro Techniques; Protein Conformation; Protein Denaturation; Protein Structure, Quaternary; Sheep; Spectrin; Tryptophan; Urea | 2005 |
Structure and stability of arthropodan hemocyanin Limulus polyphemus.
Topics: Amino Acid Sequence; Animals; Apoproteins; Binding Sites; Chromatography, Liquid; Copper; Crustacea; Guanidine; Hemocyanins; Horseshoe Crabs; Molecular Sequence Data; Protein Binding; Protein Conformation; Protein Denaturation; Protein Subunits; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Tryptophan | 2005 |
Characterization of kinetic folding intermediates of recombinant canine milk lysozyme by stopped-flow circular dichroism.
Topics: Animals; Circular Dichroism; Dogs; Guanidine; Hot Temperature; Kinetics; Milk Proteins; Models, Chemical; Models, Molecular; Muramidase; Protein Denaturation; Protein Folding; Recombinant Proteins; Tryptophan | 2005 |
Intrinsic fluorescent detection of tau conformation and aggregation.
Topics: Fluorescence Resonance Energy Transfer; Guanidine; Humans; Nerve Tissue Proteins; Protein Denaturation; tau Proteins; Tryptophan; Tyrosine | 2005 |
Main-chain dominated amyloid structures demonstrated by the effect of high pressure.
Topics: Amyloid; Benzothiazoles; beta 2-Microglobulin; Dialysis; Guanidine; Humans; Hydrostatic Pressure; Protein Conformation; Protein Denaturation; Protein Folding; Thiazoles; Tryptophan | 2005 |
Aggregation of granulocyte-colony stimulating factor in vitro involves a conformationally altered monomeric state.
Topics: Biophysical Phenomena; Biophysics; Circular Dichroism; Cysteine; Fluorescence; Granulocyte Colony-Stimulating Factor; Guanidine; Humans; Hydrogen-Ion Concentration; Models, Molecular; Protein Conformation; Protein Denaturation; Recombinant Proteins; Tryptophan | 2005 |
A close look at fluorescence quenching of organic dyes by tryptophan.
Topics: Electron Transport; Fluorescence; Fluorescent Dyes; Glycerol; Guanidine; Oxazines; Photochemistry; Spectrometry, Fluorescence; Tryptophan | 2005 |
Influence of Trp mutation on native, intermediate, and transition states of goat alpha-lactalbumin: an equilibrium and kinetic study.
Topics: Animals; Circular Dichroism; Goats; Guanidine; Hydrophobic and Hydrophilic Interactions; Kinetics; Lactalbumin; Mathematics; Point Mutation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 2005 |
Low versus high molecular weight poly(ethylene glycol)-induced states of stem bromelain at low pH: stabilization of molten globule and unfolded states.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Biopolymers; Bromelains; Circular Dichroism; Enzyme Stability; Ethylene Glycol; Guanidine; Hydrogen-Ion Concentration; Molecular Conformation; Molecular Weight; Polyethylene Glycols; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan | 2006 |
Structure and stability of apolipoprotein a-I in solution and in discoidal high-density lipoprotein probed by double charge ablation and deletion mutation.
Topics: Anilino Naphthalenesulfonates; Animals; Apolipoprotein A-I; Baculoviridae; Circular Dichroism; Dose-Response Relationship, Drug; Gene Deletion; Guanidine; Humans; Lipids; Lipoproteins, HDL; Models, Biological; Mutation; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Solutions; Spectrometry, Fluorescence; Static Electricity; Temperature; Tryptophan; Tumor Cells, Cultured | 2006 |
Fibrinogen-beta-estradiol binding studied by fluorescence spectroscopy: denaturation and pH effects.
Topics: Binding Sites; Estradiol; Fibrinogen; Guanidine; Humans; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan | 2006 |
The pressure effect on the structure and functions of protein disulfide isomerase.
Topics: Animals; Cattle; Circular Dichroism; Fluorescence; Glutathione Disulfide; Guanidine; Molecular Chaperones; Pressure; Protein Conformation; Protein Denaturation; Protein Disulfide-Isomerases; Tryptophan | 2006 |
Effect of carboxyl-terminal truncation on structure and lipid interaction of human apolipoprotein E4.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Apolipoprotein E4; Apolipoproteins E; Chromatography, Gel; Emulsions; Guanidine; Humans; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2006 |
Guanidinium derivatives bind preferentially and trigger long-distance conformational changes in an engineered T4 lysozyme.
Topics: Amino Acid Sequence; Bacteriophage T4; Crystallography, X-Ray; Dose-Response Relationship, Drug; Evolution, Molecular; Guanidine; Kinetics; Ligands; Magnetic Resonance Spectroscopy; Methylguanidine; Models, Molecular; Molecular Sequence Data; Muramidase; Protein Binding; Protein Conformation; Solutions; Thermodynamics; Tryptophan | 2006 |
[Room temperature phosphorescence of amorphous aggregates and amyloid fibrils resulting from protein misfolding].
Topics: Actins; Amyloid; Amyloidosis; Animals; Cattle; Guanidine; Insulin; Kinetics; Lactalbumin; Luminescence; Luminescent Measurements; Muscle, Skeletal; Prion Diseases; Protein Denaturation; Protein Folding; Rabbits; Temperature; Tryptophan | 2005 |
Biochemical characterization of prephenate dehydrogenase from the hyperthermophilic bacterium Aquifex aeolicus.
Topics: Bacteria; Bacterial Proteins; Binding Sites; Cloning, Molecular; Dimerization; Enzyme Stability; Fluorescence; Guanidine; Kinetics; Molecular Weight; Multienzyme Complexes; Prephenate Dehydrogenase; Protein Denaturation; Protein Folding; Protein Subunits; Tryptophan; Tyrosine | 2006 |
Phi-analysis of the folding of the B domain of protein A using multiple optical probes.
Topics: Circular Dichroism; Guanidine; Hydrogen Bonding; Kinetics; Mutation; Nuclear Magnetic Resonance, Biomolecular; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Reproducibility of Results; Staphylococcal Protein A; Staphylococcus aureus; Thermodynamics; Tryptophan | 2006 |
Effects of guanidine hydrochloride on the conformation and enzyme activity of streptomycin adenylyltransferase monitored by circular dichroism and fluorescence spectroscopy.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Circular Dichroism; Guanidine; Mycobacterium tuberculosis; Nucleotidyltransferases; Potassium Iodide; Protein Binding; Protein Conformation; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 2006 |
Guanidinium chloride and urea denaturations of beta-lactoglobulin A at pH 2.0 and 25 degrees C: the equilibrium intermediate contains non-native structures (helix, tryptophan and hydrophobic patches).
Topics: Animals; Cattle; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Kinetics; Lactoglobulins; Parasympathomimetics; Protein Denaturation; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 2007 |
Conformational changes of maize and wheat NADP-malic enzyme studied by quenching of protein native fluorescence.
Topics: Amino Acid Sequence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Malate Dehydrogenase; Molecular Sequence Data; Protein Denaturation; Protein Structure, Quaternary; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Temperature; Triticum; Tryptophan; Zea mays | 2007 |
Kinetic refolding barrier of guanidinium chloride denatured goose delta-crystallin leads to regular aggregate formation.
Topics: Animals; Benzothiazoles; Birds; Chromatography, Gel; Circular Dichroism; Congo Red; delta-Crystallins; Dimerization; Electrophoresis, Polyacrylamide Gel; Guanidine; Kinetics; Microscopy, Electron, Transmission; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Subunits; Thiazoles; Tryptophan | 2007 |
Domain organization and polarity of tail needle GP26 in the portal vertex structure of bacteriophage P22.
Topics: Alanine; Bacteriophage P22; Guanidine; Hydrophobic and Hydrophilic Interactions; Mutation; Open Reading Frames; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Transition Temperature; Tryptophan; Viral Proteins | 2007 |
Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis.
Topics: Amino Acid Sequence; Escherichia coli; Escherichia coli Proteins; GTP Phosphohydrolases; Guanidine; Guanosine Diphosphate; Guanosine Triphosphate; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Point Mutation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Structure-Activity Relationship; Tryptophan | 2007 |
Fesselin is a natively unfolded protein.
Topics: Amino Acids; Animals; Chickens; Environment; Guanidine; Hot Temperature; Hydrogen-Ion Concentration; Membrane Proteins; Microfilament Proteins; Myosin Subfragments; Proline; Protein Binding; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 2007 |
Dynamic similarity of the unfolded states of proteins L and G.
Topics: Algorithms; Bacterial Proteins; Computer Simulation; Deuterium Exchange Measurement; DNA-Binding Proteins; Fluorescence Resonance Energy Transfer; Guanidine; Hydrogen Bonding; Hydrophobic and Hydrophilic Interactions; Models, Chemical; Models, Theoretical; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Proteins; Recombinant Fusion Proteins; Thermodynamics; Tryptophan; Urea | 2007 |
Stability and dynamics of the porcine odorant-binding protein.
Topics: Animals; Circular Dichroism; Guanidine; Protein Denaturation; Protein Structure, Tertiary; Receptors, Odorant; Spectrometry, Fluorescence; Swine; Thermodynamics; Tryptophan | 2007 |
Unraveling multistate unfolding of pig kidney fructose-1,6-bisphosphatase using single tryptophan mutants.
Topics: Anilino Naphthalenesulfonates; Animals; Catalysis; Chromatography, Gel; Fructose-Bisphosphatase; Guanidine; Kidney; Kinetics; Magnesium; Mutagenesis, Site-Directed; Mutation; Protein Denaturation; Protein Folding; Protein Renaturation; Spectrometry, Fluorescence; Sulfhydryl Compounds; Sulfhydryl Reagents; Swine; Tryptophan | 2007 |
Comparison of the chemical and thermal denaturation of proteins by a two-state transition model.
Topics: Bacterial Proteins; Calorimetry, Differential Scanning; Escherichia coli Proteins; Guanidine; Haemophilus influenzae; Hot Temperature; Phosphoric Monoester Hydrolases; Protein Conformation; Protein Denaturation; Protein Folding; RNA-Binding Proteins; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Tyrosine | 2008 |
Peptide sequence and conformation strongly influence tryptophan fluorescence.
Topics: Acrylamides; Biophysics; Computational Biology; Guanidine; Iodides; Molecular Conformation; Normal Distribution; Peptides; Spectrometry, Fluorescence; Temperature; Time Factors; Tryptophan; Tyrosine; Urea | 2008 |
Toxoplasma gondii ferredoxin-NADP+ reductase: Role of ionic interactions in stabilization of native conformation and structural cooperativity.
Topics: Animals; Catalysis; Chromatography, Gel; Circular Dichroism; Enzyme Stability; Ferredoxin-NADP Reductase; Flavin-Adenine Dinucleotide; Guanidine; Hydrogen Bonding; Hydrogen-Ion Concentration; Ions; Models, Biological; Models, Molecular; Molecular Weight; NADP; Protein Binding; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Subunits; Recombinant Proteins; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Static Electricity; Temperature; Toxoplasma; Tryptophan | 2008 |
Compact dimension of denatured states of staphylococcal nuclease.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Mutant Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Salmon; Tryptophan | 2008 |
Understanding the role of Arg96 in structure and stability of green fluorescent protein.
Topics: Amino Acid Substitution; Arginine; Bacteria; Green Fluorescent Proteins; Guanidine; Kinetics; Models, Molecular; Mutant Proteins; Protein Conformation; Protein Folding; Solvents; Spectrometry, Fluorescence; Structure-Activity Relationship; Thermodynamics; Tryptophan | 2008 |
Identification of a dimeric intermediate in the unfolding pathway for the calcium-binding protein S100B.
Topics: Circular Dichroism; Dimerization; Guanidine; Humans; Models, Molecular; Molecular Weight; Nerve Growth Factors; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; S100 Calcium Binding Protein beta Subunit; S100 Proteins; Tryptophan | 2008 |
Tryptophan-tryptophan energy migration as a tool to follow apoflavodoxin folding.
Topics: Apoproteins; Azotobacter vinelandii; Bacterial Proteins; Energy Transfer; Flavodoxin; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Guanidine; Models, Molecular; Protein Conformation; Protein Folding; Time Factors; Tryptophan | 2008 |
Denaturational stress induces formation of zinc-deficient monomers of Cu,Zn superoxide dismutase: implications for pathogenesis in amyotrophic lateral sclerosis.
Topics: Amyotrophic Lateral Sclerosis; Copper; Dimerization; Guanidine; Humans; Kinetics; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Superoxide Dismutase; Thermodynamics; Tryptophan; Zinc | 2008 |
Kinetics of contact formation and end-to-end distance distributions of swollen disordered peptides.
Topics: Algorithms; Buffers; Circular Dichroism; Cysteine; Energy Transfer; Guanidine; Kinetics; Models, Chemical; Peptides; Protein Conformation; Protein Denaturation; Tryptophan; Urea | 2009 |
Fluorescence study on interactions of alpha-crystallin with the molten globule state of 1, 4-beta-D-glucan glucanohydrolase from Thermomonospora sp. induced by guanidine hydrochloride.
Topics: Actinomycetales; alpha-Crystallins; Bacterial Proteins; Circular Dichroism; Fluorescence; Glucan 1,4-beta-Glucosidase; Guanidine; Hydrophobic and Hydrophilic Interactions; Models, Chemical; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Time Factors; Tryptophan | 2009 |
Leishmania donovani trypanothione reductase: role of urea and guanidine hydrochloride in modulation of functional and structural properties.
Topics: Animals; Circular Dichroism; Dimerization; Enzyme Stability; Flavin-Adenine Dinucleotide; Guanidine; Leishmania donovani; Models, Molecular; NADH, NADPH Oxidoreductases; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Spectrometry, Fluorescence; Spectrophotometry; Sulfhydryl Compounds; Thermodynamics; Tryptophan; Urea | 2009 |
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy.
Topics: Algorithms; Anilino Naphthalenesulfonates; Animals; Cattle; Guanidine; Membrane Proteins; Models, Chemical; Models, Molecular; Nonlinear Dynamics; Normal Distribution; Protein Denaturation; Protein Folding; Protein Stability; Regression Analysis; Serum Albumin, Bovine; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2010 |
Conformational properties of unfolded HypF-N.
Topics: Carboxyl and Carbamoyl Transferases; Cysteine; Diffusion; Escherichia coli Proteins; Guanidine; Kinetics; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Tryptophan | 2009 |
Evidence for a partially structured state of the amylin monomer.
Topics: Amino Acid Sequence; Amyloid; Animals; Diffusion; Disulfides; Guanidine; Humans; Islet Amyloid Polypeptide; Kinetics; Molecular Dynamics Simulation; Molecular Sequence Data; Peptides; Protein Conformation; Protein Denaturation; Rats; Reference Standards; Tryptophan; Water | 2009 |
Conformational stability and activity of circular Enterocin AS-48 derivatives.
Topics: Algorithms; Anti-Bacterial Agents; Bacterial Proteins; Circular Dichroism; Enterococcus faecalis; Guanidine; Kinetics; Microbial Sensitivity Tests; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Peptides; Protein Conformation; Protein Folding; Protein Stability; Protein Structure, Tertiary; Structure-Activity Relationship; Temperature; Thermodynamics; Tryptophan | 2010 |
Denaturation and solvent effect on the conformation and fibril formation of TGFBIp.
Topics: Amyloid; Cell Line; Extracellular Matrix Proteins; Guanidine; Humans; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Solvents; Spectrometry, Fluorescence; Transforming Growth Factor beta; Trifluoroethanol; Tryptophan; Urea | 2009 |
Reduced stability and enhanced surface hydrophobicity drive the binding of apo-aconitase with GroEL during chaperone assisted refolding.
Topics: Aconitate Hydratase; Apoenzymes; Chaperonin 60; Circular Dichroism; Fungal Proteins; Guanidine; Holoenzymes; Hydrophobic and Hydrophilic Interactions; Iron-Sulfur Proteins; Kinetics; Osmolar Concentration; Peptide Fragments; Protein Binding; Protein Denaturation; Protein Folding; Protein Stability; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Yeasts | 2010 |
Differential scanning calorimetry and fluorescence study of lactoperoxidase as a function of guanidinium-HCl, urea, and pH.
Topics: Animals; Calorimetry; Calorimetry, Differential Scanning; Cattle; Guanidine; Hydrochloric Acid; Hydrogen-Ion Concentration; Kinetics; Lactoperoxidase; Microscopy, Fluorescence; Molecular Conformation; Temperature; Thermodynamics; Tryptophan; Urea | 2010 |
[Effects of redox state of disulfide bonds on the intrinsic fluorescence and denaturation of Trx-fused gibberellin-induced cysteine-rich protein from Gymnadnia conopsea].
Topics: Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Fluorescence; Gibberellins; Guanidine; Hydrogen-Ion Concentration; Orchidaceae; Oxidation-Reduction; Plant Proteins; Protein Conformation; Spectrometry, Fluorescence; Thermodynamics; Thioredoxins; Tryptophan | 2010 |
Specific molten globule conformation of stem bromelain at alkaline pH.
Topics: Ananas; Bromelains; Circular Dichroism; Guanidine; Hot Temperature; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Intestinal Absorption; Isoelectric Point; Phytotherapy; Plant Proteins; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2010 |
Solid-phase synthesis of new Trp(Nps)-containing dipeptide derivatives as TRPV1 channel blockers.
Topics: Alkanes; Dipeptides; Guanidine; Lysine; Structure-Activity Relationship; TRPV Cation Channels; Tryptophan | 2010 |
The role of tryptophan in staphylococcal nuclease stability.
Topics: Anilino Naphthalenesulfonates; Bromosuccinimide; Circular Dichroism; Enzyme Stability; Guanidine; Micrococcal Nuclease; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Unfolding; Spectrometry, Fluorescence; Trifluoroethanol; Tryptophan | 2010 |
Novel proton transfer fluorescence probe 2-hydroxy-pyridine and 5-(4-fluorophenyl)-2-hydroxypyridine for studying native, denatured and renatured state of protein Bovine Serum Albumin.
Topics: Animals; Cattle; Fluorescent Dyes; Guanidine; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Protons; Pyridones; Serum Albumin, Bovine; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 2010 |
Tryptophan-tryptophan energy transfer and classification of tryptophan residues in proteins using a therapeutic monoclonal antibody as a model.
Topics: Antibodies, Monoclonal; Crystallography, X-Ray; Fluorescence Resonance Energy Transfer; Guanidine; Models, Molecular; Molecular Dynamics Simulation; Protein Denaturation; Proteins; Tryptophan | 2011 |
Kinetic stability may determine the interaction dynamics of the bifunctional protein DCoH1, the dimerization cofactor of the transcription factor HNF-1α .
Topics: Chromatography, Gel; Enzyme Stability; Guanidine; Hepatocyte Nuclear Factor 1-alpha; Hydro-Lyases; Kinetics; Models, Molecular; Point Mutation; Protein Denaturation; Protein Multimerization; Protein Unfolding; Spectrometry, Fluorescence; Tryptophan | 2010 |
Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin.
Topics: Adenylate Cyclase Toxin; Bordetella pertussis; Calcium; Circular Dichroism; Fluorescence; Guanidine; Models, Molecular; Protein Folding; Protein Stability; Protein Structure, Tertiary; Sodium Chloride; Spectroscopy, Fourier Transform Infrared; Tryptophan; Urea | 2010 |
Examining the influence of ultraviolet C irradiation on recombinant human γD-crystallin.
Topics: Acrylamide; Chemical Precipitation; Circular Dichroism; Disulfides; Electrophoresis, Polyacrylamide Gel; gamma-Crystallins; Guanidine; Humans; Molecular Weight; Nephelometry and Turbidimetry; Polymerization; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Solubility; Spectrometry, Fluorescence; Tryptophan; Ultraviolet Rays | 2010 |
Sensing the dissociation of a polymeric enzyme by means of an engineered intrinsic probe.
Topics: Algorithms; Bacterial Proteins; Brucella; Guanidine; Hydrogen-Ion Concentration; Light; Multienzyme Complexes; Mutagenesis, Site-Directed; Point Mutation; Protein Folding; Protein Multimerization; Protein Stability; Recombinant Proteins; Scattering, Radiation; Spectrum Analysis; Thermodynamics; Tryptophan | 2011 |
Fluorescence correlation spectroscopy of fast chain dynamics within denatured protein L.
Topics: Bacterial Proteins; Diffusion; Electron Transport; Guanidine; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 2011 |
Structural stability and unfolding transition of β-glucosidases: a comparative investigation on isozymes from a thermo-tolerant yeast.
Topics: Alkalies; beta-Glucosidase; Calorimetry, Differential Scanning; Enzyme Stability; Guanidine; Isoenzymes; Light; Pichia; Protein Unfolding; Scattering, Radiation; Spectrometry, Fluorescence; Temperature; Tryptophan | 2011 |
Urea and guanidinium induced denaturation of a Trp-cage miniprotein.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Magnetic Resonance Spectroscopy; Models, Molecular; Protein Denaturation; Tryptophan; Urea | 2011 |
Interaction of lysozyme with gold nanorods: conformation and activity investigations.
Topics: Animals; Chickens; Circular Dichroism; Enzyme Activation; Enzyme Stability; Gold; Guanidine; Kinetics; Muramidase; Nanotubes; Protein Binding; Protein Structure, Secondary; Protein Unfolding; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Temperature; Tryptophan | 2011 |
Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point.
Topics: Absorption; Acrylamide; Amyloid; Anilino Naphthalenesulfonates; Bromelains; Circular Dichroism; Enzyme Stability; Guanidine; Isoelectric Point; Nephelometry and Turbidimetry; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Trifluoroethanol; Tryptophan | 2011 |
Biophysical studies of an NAD(P)(+)-dependent aldehyde dehydrogenase from Bacillus licheniformis.
Topics: Aldehyde Dehydrogenase; Bacillus; Biophysical Phenomena; Circular Dichroism; Guanidine; Protein Multimerization; Protein Structure, Quaternary; Protein Unfolding; Sodium Dodecyl Sulfate; Solvents; Spectrometry, Fluorescence; Temperature; Tryptophan; Ultracentrifugation; Urea | 2011 |
Microsecond unfolding kinetics of sheep prion protein reveals an intermediate that correlates with susceptibility to classical scrapie.
Topics: Animals; Disease Susceptibility; Guanidine; Humans; Hydrogen-Ion Concentration; Kinetics; Models, Biological; Models, Molecular; Prions; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Unfolding; Scrapie; Sheep; Spectrometry, Fluorescence; Tryptophan; Urea | 2011 |
Evidence of multiple folding pathways for the villin headpiece subdomain.
Topics: Amino Acid Sequence; Guanidine; Kinetics; Microfilament Proteins; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Temperature; Tryptophan | 2011 |
Influence of denaturants on native-state structural fluctuations in azurin probed by molecular size-dependent quenching of Trp phosphorescence.
Topics: Azurin; Guanidine; Protein Denaturation; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 2011 |
Tryptophan stabilizes His-heme loops in the denatured state only when it is near a loop end.
Topics: Alanine; Guanidine; Hemeproteins; Histidine; Protein Denaturation; Protein Stability; Protein Structure, Secondary; Tryptophan | 2012 |
Differing structural characteristics of molten globule intermediate of peanut lectin in urea and guanidine-HCl.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Guanidine; Peanut Agglutinin; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Protein Unfolding; Temperature; Tryptophan; Urea | 2012 |
Stabilization of ferrochelatase via lysine residues on the carboxyl terminal extension.
Topics: Acrylamide; Animals; Chironomidae; Enzyme Stability; Ferrochelatase; Guanidine; Insect Proteins; Lysine; Mutation; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Structural Homology, Protein; Tryptophan | 2013 |
Studies on the chemical modification of goat liver cystatin and the effect on its anti-papain inhibitory activity.
Topics: Absorption; Acetic Anhydrides; Acetylation; Animals; Cystatins; Dose-Response Relationship, Drug; Goats; Guanidine; Liver; Oxidation-Reduction; Papain; Protease Inhibitors; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan | 2012 |
Transient non-native burial of a Trp residue occurs initially during the unfolding of a SH3 domain.
Topics: Guanidine; Kinetics; Models, Molecular; Phosphatidylinositol 3-Kinases; Protein Denaturation; Spectrometry, Fluorescence; src Homology Domains; Tryptophan | 2012 |
Protein l-isoaspartyl-O-methyltransferase of Vibrio cholerae: interaction with cofactors and effect of osmolytes on unfolding.
Topics: Coenzymes; Glycerol; Guanidine; Methylamines; Models, Molecular; Osmosis; Protein Conformation; Protein D-Aspartate-L-Isoaspartate Methyltransferase; Protein Unfolding; S-Adenosylmethionine; Solvents; Stress, Physiological; Thermodynamics; Tryptophan; Vibrio cholerae | 2013 |
Solution and in silico studies on the recombinant lectin from Cicer arietinum seeds.
Topics: Animals; Cicer; Cloning, Molecular; Computer Simulation; Guanidine; Lectins; Models, Molecular; Molecular Sequence Data; Protein Denaturation; Protein Refolding; Protein Structure, Secondary; Protein Unfolding; Rabbits; Recombinant Proteins; Reproducibility of Results; Seeds; Solutions; Spectrometry, Fluorescence; Structural Homology, Protein; Temperature; Tryptophan | 2013 |
Chemical-genetic identification of the biochemical targets of polyalkyl guanidinium biocides.
Topics: Alkylation; Disinfectants; Gene Expression Regulation, Fungal; Guanidine; Saccharomycetales; Tryptophan | 2013 |
A comparative study of unfolding of Erythrina cristagalli Lectin in chemical denaturant and Fluoroalcohols.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Erythrina; Guanidine; Hydrogen-Ion Concentration; Oxidation-Reduction; Plant Lectins; Propanols; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Trifluoroethanol; Tryptophan | 2014 |
Mechanistic investigation of domain specific unfolding of human serum albumin and the effect of sucrose.
Topics: Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Guanidine; Hemin; Humans; Models, Molecular; Molecular Dynamics Simulation; Protein Denaturation; Protein Stability; Protein Structure, Tertiary; Protein Unfolding; Serum Albumin; Spectrometry, Fluorescence; Sucrose; Tryptophan; Water | 2013 |
Thermally stable harpin, HrpZPss is sensitive to chemical denaturants: probing tryptophan environment, chemical and thermal unfolding by fluorescence spectroscopy.
Topics: Acrylamide; Bacterial Outer Membrane Proteins; Calorimetry, Differential Scanning; Guanidine; Kinetics; Protein Denaturation; Protein Stability; Protein Unfolding; Spectrometry, Fluorescence; Tryptophan; Urea | 2013 |
Fluorescence lifetime distributions report on protein destabilisation in quenching experiments.
Topics: Circular Dichroism; Fluorescent Dyes; Guanidine; Protein Denaturation; Protein Stability; Proteins; Spectrometry, Fluorescence; Tryptophan | 2013 |
A molten globule intermediate of the von Willebrand factor A1 domain firmly tethers platelets under shear flow.
Topics: Acrylamide; Blood Platelets; Chromatography, Gel; Disulfides; Guanidine; Humans; Oxidation-Reduction; Platelet Adhesiveness; Protein Denaturation; Protein Structure, Tertiary; Rheology; Shear Strength; Spectrometry, Fluorescence; Temperature; Tryptophan; von Willebrand Factor | 2014 |
Kinetic evidence for a two-stage mechanism of protein denaturation by guanidinium chloride.
Topics: Circular Dichroism; Escherichia coli; Fluorescence; Guanidine; Kinetics; Nitrobenzoates; Protein Denaturation; Protein Structure, Secondary; Protein Unfolding; Ribonuclease H; Solvents; Sulfhydryl Compounds; Tryptophan | 2014 |
Interaction of aromatic units of amino acids with guanidinium cation: The interplay of π···π, X-H···π, and M+ ···π contacts.
Topics: Benzene; Guanidine; Histidine; Hydrogen Bonding; Indoles; Models, Molecular; Molecular Dynamics Simulation; Phenol; Phenylalanine; Static Electricity; Thermodynamics; Tryptophan | 2014 |
Chemical- and thermal-induced unfolding of Leishmania donovani ribose-5-phosphate isomerase B: a single-tryptophan protein.
Topics: Aldose-Ketose Isomerases; Enzyme Stability; Guanidine; Leishmania donovani; Protein Unfolding; Recombinant Proteins; Temperature; Tryptophan; Urea | 2014 |
Modeling the solvation of nonpolar amino acids in guanidinium chloride solutions.
Topics: Amino Acids; Entropy; Guanidine; Ions; Linear Models; Models, Chemical; Molecular Structure; Nonlinear Dynamics; Protein Denaturation; Solutions; Solvents; Temperature; Thermodynamics; Tryptophan; Water | 2014 |
Residues Phe103 and Phe149 are critical for the co-chaperone activity of Bacillus licheniformis GrpE.
Topics: Amino Acid Sequence; Bacillus; Bacterial Proteins; Circular Dichroism; Gene Expression Regulation, Bacterial; Guanidine; Heat-Shock Proteins; Molecular Chaperones; Mutagenesis, Site-Directed; Mutant Proteins; Protein Folding; Protein Structure, Secondary; Thermodynamics; Tryptophan | 2015 |
Fluorescence study of domain structure and lipid interaction of human apolipoproteins E3 and E4.
Topics: 2-Naphthylamine; Animals; Apolipoprotein E3; Apolipoprotein E4; Chickens; Fluorescence Resonance Energy Transfer; Guanidine; Humans; Kinetics; Lipids; Phosphatidylcholines; Protein Denaturation; Protein Isoforms; Protein Stability; Protein Structure, Tertiary; Pyrenes; Time Factors; Tryptophan; Unilamellar Liposomes | 2014 |
Residual structures in the unfolded state of starch-binding domain of glucoamylase revealed by near-UV circular dichroism and protein engineering techniques.
Topics: Amino Acid Sequence; Aspergillus niger; Circular Dichroism; Fluorescence; Glucan 1,4-alpha-Glucosidase; Guanidine; Hydrophobic and Hydrophilic Interactions; Kinetics; Protein Binding; Protein Denaturation; Protein Domains; Protein Engineering; Protein Folding; Spectrometry, Fluorescence; Starch; Thermodynamics; Tryptophan; Ultraviolet Rays | 2016 |
Application of Tryptophan Fluorescence Bandwidth-Maximum Plot in Analysis of Monoclonal Antibody Structure.
Topics: Antibodies, Monoclonal; Fluorescence; Guanidine; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2017 |
Fluorescence and circular dichroism studies on the accessibility of tryptophan residues and unfolding of a jacalin-related α-d-galactose-specific lectin from mulberry (Morus indica).
Topics: Cesium; Circular Dichroism; Galactose; Guanidine; Guanidines; Hydrogen-Ion Concentration; Iodides; Lectins; Morus; Plant Lectins; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Unfolding; Spectrometry, Fluorescence; Temperature; Thiocyanates; Tryptophan; Urea | 2017 |
Fluorescence quenching, structural and unfolding studies of a purified cysteine protease, ZCPG from Zingiber montanum rhizome.
Topics: Amino Acid Motifs; Cysteine Proteases; Guanidine; Hydrogen-Ion Concentration; Phenylalanine; Plant Proteins; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Rhizome; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Zingiberaceae | 2018 |
Protein Aggregation and Performance Optimization Based on Microconformational Changes of Aromatic Hydrophobic Regions.
Topics: Arginine; Chemistry, Pharmaceutical; Guanidine; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Protein Aggregates; Protein Conformation; Proteins; Solubility; Surface Properties; Tryptophan | 2018 |
Tracking Zone-wise perturbation during unfolding of some globular proteins using Eu(III) complex of Tetracycline as a probe exhibiting Stark splitting.
Topics: Guanidine; Humans; Protein Denaturation; Spectrometry, Fluorescence; Tetracycline; Tryptophan | 2022 |
Potential Anti-Alzheimer Agents from Guanidinyl Tryptophan Derivatives with Activities of Membrane Adhesion and Conformational Transition Inhibitions.
Topics: Adhesiveness; Adsorption; Alzheimer Disease; Amyloid beta-Peptides; Cell Membrane; Guanidine; Humans; Ligands; Lipid Bilayers; Lipids; Models, Molecular; Molecular Dynamics Simulation; Phosphatidylcholines; Protein Conformation; Protein Structure, Secondary; Tryptophan; Water | 2021 |
Classification of protein solubilizing solutes by fluorescence assay.
Topics: Fluorescence; Guanidine; Proteins; Solutions; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 2022 |