trisialoganglioside-gt1 and purmorphamine

trisialoganglioside-gt1 has been researched along with purmorphamine* in 2 studies

Other Studies

2 other study(ies) available for trisialoganglioside-gt1 and purmorphamine

Article	Year
Development of a Highly Sensitive Cell-Based Assay for Detecting Botulinum Neurotoxin Type A through Neural Culture Media Optimization. Journal of biomolecular screening, 2016, Volume: 21, Issue:1 Botulinum neurotoxin (BoNT) is the most lethal naturally produced neurotoxin. Due to the extreme toxicity, BoNTs are implicated in bioterrorism, while the specific mechanism of action and long-lasting effect was found to be medically applicable in treating various neurological disorders. Therefore, for both public and patient safety, a highly sensitive, physiologic, and specific assay is needed. In this paper, we show a method for achieving a highly sensitive cell-based assay for BoNT/A detection using the motor neuron-like continuous cell line NG108-15. To achieve high sensitivity, we performed a media optimization study evaluating three commercially available neural supplements in combination with retinoic acid, purmorphamine, transforming growth factor β1 (TGFβ1), and ganglioside GT1b. We found nonlinear combinatorial effects on BoNT/A detection sensitivity, achieving an EC50 of 7.4 U ± 1.5 SD (or ~7.9 pM). The achieved detection sensitivity is comparable to that of assays that used primary and stem cell-derived neurons as well as the mouse lethality assay. Topics: Animals; Biological Assay; Botulinum Toxins, Type A; Cell Line, Tumor; Culture Media; Embryonic Stem Cells; Gangliosides; Mice; Morpholines; Motor Neurons; Neurotoxins; Purines; Rats; Sensitivity and Specificity; Transforming Growth Factor beta1; Tretinoin	2016
Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells. Biochemical and biophysical research communications, 2012, Oct-19, Volume: 427, Issue:2 Cancerous cell lines have traditionally shown low sensitivity to laboratory or pharmaceutical preparations of botulinum neurotoxin. The work presented here demonstrates that the mouse neuroblastoma/rat glioma hybrid cell line NG108-15 is capable of more sensitively detecting BoNT/A1 than any cell line previously described. This cell line has previously been described to have motor neuron like characteristics, therefore making it a good model to study BoNTs. Differentiation of NG108-15 cells in serum-free medium containing retinoic acid and purmorphamine dramatically increased sensitivity of the neurons to BoNT/A (EC(50) = ~16 LD(50) U). Additional pre-treatment with triasialoganglioside GT1B prior to toxin exposure reduced the EC(50) further to ~11 LD(50) U. Co-culture of the neurons with C2C12 myotubes also significantly increased BoNT/A sensitivity of NG108-15 cells (EC(50) = 26 U) in the absence of differentiation factors. Topics: Animals; Apoptosis; Botulinum Toxins, Type A; Cell Differentiation; Cholinergic Neurons; Clostridium botulinum; Coculture Techniques; Gangliosides; Mice; Models, Neurological; Morpholines; Motor Neurons; Neurotoxins; Purines; Rats; Tretinoin	2012

Article

Year

Development of a Highly Sensitive Cell-Based Assay for Detecting Botulinum Neurotoxin Type A through Neural Culture Media Optimization.

Journal of biomolecular screening, 2016, Volume: 21, Issue:1

Botulinum neurotoxin (BoNT) is the most lethal naturally produced neurotoxin. Due to the extreme toxicity, BoNTs are implicated in bioterrorism, while the specific mechanism of action and long-lasting effect was found to be medically applicable in treating various neurological disorders. Therefore, for both public and patient safety, a highly sensitive, physiologic, and specific assay is needed. In this paper, we show a method for achieving a highly sensitive cell-based assay for BoNT/A detection using the motor neuron-like continuous cell line NG108-15. To achieve high sensitivity, we performed a media optimization study evaluating three commercially available neural supplements in combination with retinoic acid, purmorphamine, transforming growth factor β1 (TGFβ1), and ganglioside GT1b. We found nonlinear combinatorial effects on BoNT/A detection sensitivity, achieving an EC50 of 7.4 U ± 1.5 SD (or ~7.9 pM). The achieved detection sensitivity is comparable to that of assays that used primary and stem cell-derived neurons as well as the mouse lethality assay.

Topics: Animals; Biological Assay; Botulinum Toxins, Type A; Cell Line, Tumor; Culture Media; Embryonic Stem Cells; Gangliosides; Mice; Morpholines; Motor Neurons; Neurotoxins; Purines; Rats; Sensitivity and Specificity; Transforming Growth Factor beta1; Tretinoin

2016

Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells.

Biochemical and biophysical research communications, 2012, Oct-19, Volume: 427, Issue:2

Cancerous cell lines have traditionally shown low sensitivity to laboratory or pharmaceutical preparations of botulinum neurotoxin. The work presented here demonstrates that the mouse neuroblastoma/rat glioma hybrid cell line NG108-15 is capable of more sensitively detecting BoNT/A1 than any cell line previously described. This cell line has previously been described to have motor neuron like characteristics, therefore making it a good model to study BoNTs. Differentiation of NG108-15 cells in serum-free medium containing retinoic acid and purmorphamine dramatically increased sensitivity of the neurons to BoNT/A (EC(50) = ~16 LD(50) U). Additional pre-treatment with triasialoganglioside GT1B prior to toxin exposure reduced the EC(50) further to ~11 LD(50) U. Co-culture of the neurons with C2C12 myotubes also significantly increased BoNT/A sensitivity of NG108-15 cells (EC(50) = 26 U) in the absence of differentiation factors.

Topics: Animals; Apoptosis; Botulinum Toxins, Type A; Cell Differentiation; Cholinergic Neurons; Clostridium botulinum; Coculture Techniques; Gangliosides; Mice; Models, Neurological; Morpholines; Motor Neurons; Neurotoxins; Purines; Rats; Tretinoin

2012