tricin and daidzein

Rhynchosia minima root, a folk herbal medicine in southern China, is used to relieve itch and swelling. In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) from R. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism. The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry. The result showed that five flavonoids compounds, 2',4',5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6. In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF-κB signaling pathways by decreasing the secretion of nitric oxide (NO), interleukin (IL)-6, TNF-α, and monocyte chemotactic protein (MCP)-1, inhibiting the translocation of p65 from cytoplasm to nucleus, and suppressing the phosphorylation of ERK, JNK, and p38. These results indicated that EEF6 could be a promising ingredient for inflammation management.

Topics: Animals; Anti-Inflammatory Agents; Cell Line; Cytokines; Fabaceae; Flavonoids; Genistein; Inflammation; Interleukin-6; Isoflavones; Lipopolysaccharides; Macrophages; MAP Kinase Signaling System; Mice; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; Phosphorylation; Plant Roots; RAW 264.7 Cells; Signal Transduction; Tumor Necrosis Factor-alpha