tretinoin and tetrandrine

To investigate the differentiation of acute promyelocytic leukemia (APL) cells induced by Tetrandrine targeting MUC1.. HL-60 cells at logarithmic growth phase were choosen as object of reaserch and were divided into four groups: control, ATRA, Tetrandrine and ATRA+Tetrandrine group. Cell morphologic changes in each group were observed by microscopy. The change of cell differentiation ability was analyzed by nitro-blue tetrazolium (NBT) reduction test. The expression of cell surface differentiation antigen CD11b was measured by flow cytometry. The expression of MUC1 protein was detected by Western blot.. The differentiation of HL-60 cell could be induced by Tetrandrine. The NBT reduction-positive rate of ATRA+Tetrandrine group was significantly higher than that in ATRA group and Tetrandrine group (P<0.05). The percentage of CD11b positive cells in ATRA+Tetrandrine group(43.62％±1.38％) was significantly higher than that in Tetrandrine group(15.25％±2.11％), ATRA group (28.84％±7.53％) and control group(8.16％±1.01％) (P<0.05). The content of MUC1 protein in Tetrandrine group was significantly lower than that in control group and ATRA group (P<0.05).. Tetrandrine and ATRA can synergize to promote the differentiation and maturation of HL-60 cells, and the mechanism may be related with MUC1 expression.. 汉防己甲素诱导HL-60细胞分化.. 探讨汉防己甲素(Tetrandrine)靶向黏蛋白1（MUC1）诱导急性早幼粒细胞白血病(APL)细胞分化.. 以HL-60细胞株作为研究对象，选取对数生长期细胞，分为空白对照、ATRA、汉防己甲素和ATRA+汉防己甲素，共4组。显微镜下观察各组细胞形态的变化；用NBT还原实验分析细胞分化能力的变化；采用流式细胞术检测细胞表面分化抗原CD11b表达的变化；采用Western blot 检测MUC1表达的变化.. 汉防己甲素可以诱导HL-60细胞分化； ATRA+汉防己甲素组NBT还原阳性率明显高于ATRA和汉防己甲素组（P＜0.05）；ATRA+汉防己甲素组CD11b+ 细胞百分率(43.62％±1.38％) 明显高于汉防己甲素(15.25％±2.11％)、ATRA(2184％±7.53％)和空白对照组(8.16％±1.01％)（P＜0.05）；汉防己甲素组MUC1蛋白量明显低于空白对照组和ATRA组（P＜0.05）.. 汉防己甲素体外能协同ATRA使HL-60细胞分化成熟，其作用机制可能与调控MUC1的表达有关.

Topics: Benzylisoquinolines; Cell Differentiation; HL-60 Cells; Humans; Leukemia, Promyelocytic, Acute; Tretinoin