tretinoin and paclobutrazol

Paclobutrazol (PBZ) is a widely used fungicide that shows toxicity to aquatic embryos, probably through rain-wash. Here, we specifically focus on its toxic effect on eye development in zebrafish, as well as the role of retinoic acid (RA), a metabolite of vitamin A that controls proliferation and differentiation of retinal photoreceptor cells, in this toxicity. Embryos were exposed to PBZ with or without RA from 2 to 72 h post-fertilization (hpf), and PBZ-treated embryos (2-72 hpf) were exposed to RA for additional hours until 120 hpf. Eye size and histology were examined. Expression levels of

Topics: Animals; Cell Differentiation; Dose-Response Relationship, Drug; Embryo, Nonmammalian; Neuroprotective Agents; Phenotype; Photoreceptor Cells, Vertebrate; Retinal Pigment Epithelium; Tretinoin; Triazoles; Zebrafish