tretinoin and bisantrene

High performance liquid chromatographic procedures are described for melphalan, bisantrene, and 13-cis retinoic acid, three important anticancer drugs in various stages of clinical development. The procedures require a rapid and simple sample clean-up followed by a 10-to 20-min chromatographic separation on a reversed-phase C18 column. Precisions are all less than 8% with recoveries greater than 80%. Mass spectrometry confirmation of each drug from patient sample separations is presented to provide unambiguous identification for valid pharmacokinetic parameter determination.

Topics: Anthracenes; Antineoplastic Agents; Chromatography, High Pressure Liquid; Humans; Isotretinoin; Kinetics; Mass Spectrometry; Melphalan; Tretinoin