to-pro-1 and 5-carboxyfluorescein-diacetate-acetoxymethyl-ester

to-pro-1 has been researched along with 5-carboxyfluorescein-diacetate-acetoxymethyl-ester* in 1 studies

Other Studies

1 other study(ies) available for to-pro-1 and 5-carboxyfluorescein-diacetate-acetoxymethyl-ester

Article	Year
A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. Journal of microbiological methods, 2012, Volume: 89, Issue:3 Two fluorescent dyes, TO-PRO-1 iodide and 5-CFDA-AM, were evaluated for LIVE/DEAD assessment of unicellular marine algae Brachiomonas submarina and Tetraselmis suecica. Epifluorescence microscopy was used to estimate cell viability in predetermined mixtures of viable and non-viable algal cells and validated using microplate growth assay as reference measurements. On average, 5-CFDA-AM underestimated live cell abundance by ~25% compared with viability estimated by the growth assay, whereas TO-PRO-1 iodide provided accurate viability estimates. Furthermore, viability estimates based on staining with TO-PRO-1 iodide were not affected by a storage period of up to one month in -80°C, making the assay a good candidate for routine assessment of phytoplankton populations in field and laboratory studies. Topics: Cell Survival; Chlorophyta; Fluoresceins; Fluorescent Dyes; Microscopy, Fluorescence; Organic Chemicals; Staining and Labeling	2012

Article

Year

A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy.

Journal of microbiological methods, 2012, Volume: 89, Issue:3

Two fluorescent dyes, TO-PRO-1 iodide and 5-CFDA-AM, were evaluated for LIVE/DEAD assessment of unicellular marine algae Brachiomonas submarina and Tetraselmis suecica. Epifluorescence microscopy was used to estimate cell viability in predetermined mixtures of viable and non-viable algal cells and validated using microplate growth assay as reference measurements. On average, 5-CFDA-AM underestimated live cell abundance by ~25% compared with viability estimated by the growth assay, whereas TO-PRO-1 iodide provided accurate viability estimates. Furthermore, viability estimates based on staining with TO-PRO-1 iodide were not affected by a storage period of up to one month in -80°C, making the assay a good candidate for routine assessment of phytoplankton populations in field and laboratory studies.

Topics: Cell Survival; Chlorophyta; Fluoresceins; Fluorescent Dyes; Microscopy, Fluorescence; Organic Chemicals; Staining and Labeling

2012