thuringiensin and formic-acid

thuringiensin has been researched along with formic-acid* in 2 studies

Other Studies

2 other study(ies) available for thuringiensin and formic-acid

Article	Year
A fundamental dual regulatory role of citrate on the biosyntheses of thuringiensin and poly-beta-hydroxybutyrate in Bacillus thuringiensis YBT-032. Biotechnology letters, 2007, Volume: 29, Issue:5 The production of alpha-ketoglutarate, adenine, thuringiensin production rate and thuringiensin yield on glucose consumed increased by 22%, 36%, 40% and 40%, respectively, in presence of 2 g citrate/l. However, citrate decreased pyruvate production, poly-beta-hydroxybutyrate (PHB) production rate and PHB yield by 62%, 31% and 45%, respectively. The activities of pyruvate kinase and glucose-6-phosphate dehydrogenase were 36%-45% lower and 50%-120% higher than those of the control, respectively. The results suggest that citrate regulated the carbon flux to synthesis of adenine present in thuringiensin with a higher efficiency of utilization of glucose by decreasing PHB synthesis. Topics: Adenosine; Bacillus thuringiensis; Citrates; Formates; Hydroxybutyrates; Ketoglutaric Acids; Polyesters; Sugar Acids; Time Factors	2007
A fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032. Bioprocess and biosystems engineering, 2007, Volume: 30, Issue:4 In this work, a fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032 was investigated. Nicotinamide adenine dinucleotide (NADH) production and formate dehydrogenase activity increased with formate addition from 0.5 to 2.0 g/L, respectively. However, with the formate addition of 1.5 g/L, the activities of pyruvate kinase and glucose 6-phosphate dehydrogenase reached a peak and increased by 316 and 150% relative to those of the control, respectively. In addition, intracellular production of pyruvate, aspartate, citrate and adenine were significantly enhanced by 75, 66, 32 and 78% as well. An improvement (90%) of thuringiensin production was also successfully obtained. Interestingly to point out, thuringiensin yield was closely correlative with adenine production, and the linear relationship was also observed. The results suggest that appropriate formate addition did act as a modulator and facilitate carbon flux in glycolysis and pentose phosphate pathway to synthesize adenine and thuringiensin via intracellular NADH availability. Topics: Adenine; Adenosine; Bacillus thuringiensis; Cell Line; Dose-Response Relationship, Drug; Formates; Gene Expression Regulation, Bacterial; Glycolysis; Pentose Phosphate Pathway; Signal Transduction; Sugar Acids	2007

Article

Year

A fundamental dual regulatory role of citrate on the biosyntheses of thuringiensin and poly-beta-hydroxybutyrate in Bacillus thuringiensis YBT-032.

Biotechnology letters, 2007, Volume: 29, Issue:5

The production of alpha-ketoglutarate, adenine, thuringiensin production rate and thuringiensin yield on glucose consumed increased by 22%, 36%, 40% and 40%, respectively, in presence of 2 g citrate/l. However, citrate decreased pyruvate production, poly-beta-hydroxybutyrate (PHB) production rate and PHB yield by 62%, 31% and 45%, respectively. The activities of pyruvate kinase and glucose-6-phosphate dehydrogenase were 36%-45% lower and 50%-120% higher than those of the control, respectively. The results suggest that citrate regulated the carbon flux to synthesis of adenine present in thuringiensin with a higher efficiency of utilization of glucose by decreasing PHB synthesis.

Topics: Adenosine; Bacillus thuringiensis; Citrates; Formates; Hydroxybutyrates; Ketoglutaric Acids; Polyesters; Sugar Acids; Time Factors

2007

A fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032.

Bioprocess and biosystems engineering, 2007, Volume: 30, Issue:4

In this work, a fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032 was investigated. Nicotinamide adenine dinucleotide (NADH) production and formate dehydrogenase activity increased with formate addition from 0.5 to 2.0 g/L, respectively. However, with the formate addition of 1.5 g/L, the activities of pyruvate kinase and glucose 6-phosphate dehydrogenase reached a peak and increased by 316 and 150% relative to those of the control, respectively. In addition, intracellular production of pyruvate, aspartate, citrate and adenine were significantly enhanced by 75, 66, 32 and 78% as well. An improvement (90%) of thuringiensin production was also successfully obtained. Interestingly to point out, thuringiensin yield was closely correlative with adenine production, and the linear relationship was also observed. The results suggest that appropriate formate addition did act as a modulator and facilitate carbon flux in glycolysis and pentose phosphate pathway to synthesize adenine and thuringiensin via intracellular NADH availability.

Topics: Adenine; Adenosine; Bacillus thuringiensis; Cell Line; Dose-Response Relationship, Drug; Formates; Gene Expression Regulation, Bacterial; Glycolysis; Pentose Phosphate Pathway; Signal Transduction; Sugar Acids

2007