thromboxane-b2 and apigetrin

Apigenin 7-O-beta-D-glucopyranuronide (1), luteolin 7-O-beta-D-glucopyranuronide (2), m-hydroxybenzyl beta-D-glucoside (3), and chrysoeriol 7-O-beta-D-glucopyranuronide (4) were isolated for the first time from the leaves of Salix matsudana. Furthermore, the effects of compounds 1, 2 and 3 on arachidonic acid metabolism were studied. These compounds inhibited significantly the production of 12-hydroxy-5, 8, 10, 14-eicosatetraenoic acid (12-HETE). In addition, the aglycon apigenin inhibited not only 12-HETE but also thromboxane B(2) (TXB(2)). The effect of compound (4) on arachidonic acid metabolism is now under investigation.

Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Animals; Apigenin; Arachidonic Acid; Blood Platelets; Cells, Cultured; Depression, Chemical; Flavones; Flavonoids; Glucosides; Luteolin; Male; Plant Leaves; Rats; Rats, Wistar; Salix; Structure-Activity Relationship; Thromboxane B2

In this paper, three compounds were isolated and identified from the leaves of Salix matsudana. They are apigenin-7-0-beta-D-glucopyranside(I), luteolin-7-0-beta-D-glucopyranside(II), compound III. Compound I and II are isolated firstly from Salix spp., compound III is found firstly in the world. Furthermore, study on effect of arachidonic acid metabolisin in rat platelets by them with radio-chromatography found that they can significantly inhibit the production of 12-HETE(12-hydroxy-5,8, 10,14-eicosatetraenoic acid), which can induce allergy and arteriosclerosis. The production of apigenin-7-0-beta-D-glucopyranside being hydrolyzed was apigenin, it can inhibit TXB2(thromoxane B2) which can induce platelet aggregation.

Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Animals; Apigenin; Arachidonic Acid; Arteriosclerosis; Blood Platelets; Drugs, Chinese Herbal; Fibrinolytic Agents; Glucosides; Luteolin; Plant Leaves; Platelet Aggregation; Rats; Salix; Thromboxane B2