thromboxane-a2 and 8-bromoadenosine-3--5--cyclic-monophosphorothioate

thromboxane-a2 has been researched along with 8-bromoadenosine-3--5--cyclic-monophosphorothioate* in 1 studies

Other Studies

1 other study(ies) available for thromboxane-a2 and 8-bromoadenosine-3--5--cyclic-monophosphorothioate

Article	Year
Cordycepin-enriched WIB801C from Cordyceps militaris inhibits ADP-induced [Ca(2+)] i mobilization and fibrinogen binding via phosphorylation of IP 3R and VASP. Archives of pharmacal research, 2015, Volume: 38, Issue:1 In this study, we investigated the effect of cordycepin-enriched (CE)-WIB801C from Cordyceps militaris on ADP (20 µM)-stimulated platelet aggregation. CE-WIB801C dose-dependently inhibited ADP-induced platelet aggregation, and its IC50 value was 18.5 μg/mL. CE-WIB801C decreased TXA2 production, but did not inhibit the activities of COX-1 and thromboxane synthase (TXAS) in ADP-activated platelets, which suggests that the inhibition of TXA2 production by CE-WIB801C is not resulted from the direct inhibition of COX-1 and TXAS. CE-WIB801C inhibited ATP release and [Ca(2+)]i mobilization, and increased cAMP level and IP3RI (Ser(1756)) phosphorylation in ADP-activated platelets. cAMP-dependent protein kinase (A-kinase) inhibitor Rp-8-Br-cAMPS increased CE-WIB801C-inhibited [Ca(2+)]i mobilization, and strongly inhibited CE-WIB801C-increased IP3RI (Ser(1756)) phosphorylation. CE-WIB801C elevated the phosphorylation of VASP (Ser(157)), an A-kinase substrate, but inhibited fibrinogen binding to αIIb/β3. These results suggest that CE-WIB801C-elevated cAMP involved in IP3RI (Ser(1756)) phosphorylation to inhibit [Ca(2+)]i mobilization and, VASP (Ser(157)) phosphorylation to inhibit αIIb/β3 activation. Therefore, in this study, we demonstrate that CE-WIB801C may have a preventive or therapeutic potential for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease. Topics: 8-Bromo Cyclic Adenosine Monophosphate; Adenosine Diphosphate; Adenosine Triphosphate; Blood Platelets; Calcium; Calcium Signaling; Cell Adhesion Molecules; Cordyceps; Cyclic AMP; Cyclooxygenase 1; Deoxyadenosines; Dose-Response Relationship, Drug; Fibrinogen; Herb-Drug Interactions; Humans; Inositol 1,4,5-Trisphosphate Receptors; Microfilament Proteins; Phosphoproteins; Phosphorylation; Plant Extracts; Platelet Aggregation; Platelet Aggregation Inhibitors; Thionucleotides; Thromboxane A2; Thromboxane-A Synthase	2015

Article

Year

Cordycepin-enriched WIB801C from Cordyceps militaris inhibits ADP-induced [Ca(2+)] i mobilization and fibrinogen binding via phosphorylation of IP 3R and VASP.

Archives of pharmacal research, 2015, Volume: 38, Issue:1

In this study, we investigated the effect of cordycepin-enriched (CE)-WIB801C from Cordyceps militaris on ADP (20 µM)-stimulated platelet aggregation. CE-WIB801C dose-dependently inhibited ADP-induced platelet aggregation, and its IC50 value was 18.5 μg/mL. CE-WIB801C decreased TXA2 production, but did not inhibit the activities of COX-1 and thromboxane synthase (TXAS) in ADP-activated platelets, which suggests that the inhibition of TXA2 production by CE-WIB801C is not resulted from the direct inhibition of COX-1 and TXAS. CE-WIB801C inhibited ATP release and [Ca(2+)]i mobilization, and increased cAMP level and IP3RI (Ser(1756)) phosphorylation in ADP-activated platelets. cAMP-dependent protein kinase (A-kinase) inhibitor Rp-8-Br-cAMPS increased CE-WIB801C-inhibited [Ca(2+)]i mobilization, and strongly inhibited CE-WIB801C-increased IP3RI (Ser(1756)) phosphorylation. CE-WIB801C elevated the phosphorylation of VASP (Ser(157)), an A-kinase substrate, but inhibited fibrinogen binding to αIIb/β3. These results suggest that CE-WIB801C-elevated cAMP involved in IP3RI (Ser(1756)) phosphorylation to inhibit [Ca(2+)]i mobilization and, VASP (Ser(157)) phosphorylation to inhibit αIIb/β3 activation. Therefore, in this study, we demonstrate that CE-WIB801C may have a preventive or therapeutic potential for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.

Topics: 8-Bromo Cyclic Adenosine Monophosphate; Adenosine Diphosphate; Adenosine Triphosphate; Blood Platelets; Calcium; Calcium Signaling; Cell Adhesion Molecules; Cordyceps; Cyclic AMP; Cyclooxygenase 1; Deoxyadenosines; Dose-Response Relationship, Drug; Fibrinogen; Herb-Drug Interactions; Humans; Inositol 1,4,5-Trisphosphate Receptors; Microfilament Proteins; Phosphoproteins; Phosphorylation; Plant Extracts; Platelet Aggregation; Platelet Aggregation Inhibitors; Thionucleotides; Thromboxane A2; Thromboxane-A Synthase

2015