thromboxane-a2 and 4-hydroxy-2-nonenal

thromboxane-a2 has been researched along with 4-hydroxy-2-nonenal* in 1 studies

Other Studies

1 other study(ies) available for thromboxane-a2 and 4-hydroxy-2-nonenal

Article	Year
4-Hydroxynonenal induces membrane perturbations and inhibition of basal prostacyclin production in endothelial cells, and migration of monocytes. Cell biology international, 1994, Volume: 18, Issue:10 Cultured bovine aortic endothelial cells (BAEC) were incubated for 5 days with 10(-5) 4-hydroxynonenal (HN). HN treated BAEC and controls were either (i) further incubated with 125I-polymyxin B (IPxB) or with radioiodinated, inactivated coagulation factor Xa (IFXai) as markers of membrane phospholipid perturbation, or (ii) assayed for the synthesis of prostacyclin (PGI2) and thromboxane A2 (TXA2). Rabbit blood mononuclear cells enriched in monocytes (MC) were isolated and assayed for chemotactic response to HN. The results showed six - fold increases of IPxB and IFXai binding to BAEC treated with HN, as compared to untreated controls. We also found in HN treated cells a marked inhibition of PGI2 synthesis, but an unmodified TXA2 production. In addition, HN in the 10(-5)-10(-10) M range induced oriented migration of MC. Topics: Aldehydes; Animals; Arteriosclerosis; Cattle; Cells, Cultured; Chemotaxis, Leukocyte; Endothelium, Vascular; Epoprostenol; Female; In Vitro Techniques; Lipid Peroxidation; Membrane Lipids; Monocytes; Pregnancy; Thromboxane A2	1994

Article

Year

4-Hydroxynonenal induces membrane perturbations and inhibition of basal prostacyclin production in endothelial cells, and migration of monocytes.

Cell biology international, 1994, Volume: 18, Issue:10

Cultured bovine aortic endothelial cells (BAEC) were incubated for 5 days with 10(-5) 4-hydroxynonenal (HN). HN treated BAEC and controls were either (i) further incubated with 125I-polymyxin B (IPxB) or with radioiodinated, inactivated coagulation factor Xa (IFXai) as markers of membrane phospholipid perturbation, or (ii) assayed for the synthesis of prostacyclin (PGI2) and thromboxane A2 (TXA2). Rabbit blood mononuclear cells enriched in monocytes (MC) were isolated and assayed for chemotactic response to HN. The results showed six - fold increases of IPxB and IFXai binding to BAEC treated with HN, as compared to untreated controls. We also found in HN treated cells a marked inhibition of PGI2 synthesis, but an unmodified TXA2 production. In addition, HN in the 10(-5)-10(-10) M range induced oriented migration of MC.

Topics: Aldehydes; Animals; Arteriosclerosis; Cattle; Cells, Cultured; Chemotaxis, Leukocyte; Endothelium, Vascular; Epoprostenol; Female; In Vitro Techniques; Lipid Peroxidation; Membrane Lipids; Monocytes; Pregnancy; Thromboxane A2

1994