thromboplastin and methylglucoside

We purified the urinary procoagulant from frozen human urine by introducing phenyl-Sepharose hydrophobic chromatography. By this method, the apoprotein of the procoagulant and the lipid-like substance were separately recovered. Upon reassociation with the lipid-like substance or exogenous crude phospholipids, the apoprotein accelerated factor VIIa-catalyzed activation of factor X, probably by forming a stoichiometric complex with the catalytic enzyme. Thus the procoagulant was confirmed to be a tissue factor by its mode of participation in the blood coagulation mechanism.

Topics: Apoproteins; Blood Coagulation; Concanavalin A; Factor VII; Factor VIIa; Factor X; Humans; In Vitro Techniques; Male; Methylglucosides; Phospholipids; Thromboplastin