thromboplastin and 2-oxothiazolidine-4-carboxylic-acid

Peritoneal adhesions are the most common complication of the abdominal surgery. Normal saline is frequently used to rinse the peritoneal cavity during abdominal surgery, although there is no well-established data describing effect of such procedure on the process of formation of peritoneal adhesions.. Effect of 0.9% NaCl solution on viability, oxidative stress, and fibrinolytic activity of human peritoneal mesothelial cells maintained in in vitro culture was evaluated.. Exposure of mesothelial cells to 0.9% NaCl induces oxidative stress, derangement of their structure with subsequent increased release of tissue factor (+75%) and plasminogen activator inhibitor-1 (+19%), and simultaneous suppression of tissue plasminogen activator release (-39%). In effect, ration tissue plasminogen activator/plasminogen activator inhibitor-1 was reduced in 0.9% NaCl-treated cells by 50%. Pretreatment of cells with precursor of glutathione synthesis: L-2-oxothiazolidine-4-carboxylic acid prevented these changes.. Oxidative stress in the peritoneal mesothelium caused by 0.9% NaCl activates their procoagulant activity and impairs fibrinolytic properties of these cells. These effects disqualify 0.9% NaCl as rinsing solution during abdominal surgery.

Topics: Cells, Cultured; Contraindications; Epithelial Cells; Fibrinolysis; Glutathione; Humans; Omentum; Osmolar Concentration; Oxidative Stress; Peritoneal Cavity; Peritoneal Diseases; Pyrrolidonecarboxylic Acid; Reactive Oxygen Species; Sodium Chloride; Solutions; Therapeutic Irrigation; Thiazolidines; Thromboplastin; Tissue Adhesions