thiourea and phenylhydrazine

Ufmylation was proved to play a crucial role in hematopoietic stem cell (HSC) survival and erythroid differentiation, ufmylation deficiency induces acute anemia and lethality of embryos and adults in mouse models. To screen some compounds to rescue phenotypes induced by gene deletion, in this study, we used DDRGK1

Topics: Anemia; Animals; Cinnamates; Erythrocytes; Erythropoiesis; Eukaryotic Initiation Factor-2; Fetus; Hemolysis; Liver; Male; Mice, Inbred C57BL; Mice, Knockout; Models, Biological; Phenotype; Phenylhydrazines; Phosphorylation; Protein Processing, Post-Translational; Thiourea; Up-Regulation

Sixteen novel pyrazole acyl thiourea derivatives 6 were synthesized from monomethylhydrazine (phenylhydrazine) and ethyl acetoacetate. The key 5-chloro-3-methyl-1-substituted-1H-pyrazole-4-carbonyl chloride intermediates 4 were first generated in four steps through cyclization, formylation, oxidation and acylation. Thess were then reacted with ammonium thiocyanate in the presence of PEG-400 to afford 5-chloro-3-methyl-1-substituted-1H-pyrazole-4-carbonyl isothiocyanates 5. Subsequent reaction with fluorinated aromatic amines resulted in the formation of the title compounds. The synthesized compound were unequivocally characterized by IR, ¹H-NMR, ¹³C-NMR and elemental analysis and some of the synthesized compounds displayed good antifungal activities against Gibberella zeae, Fusarium oxysporum, Cytospora mandshurica and anti-TMV activity in preliminary antifungal activity tests.

Topics: Acetoacetates; Acylation; Antifungal Agents; Antiviral Agents; Cyclization; Fusarium; Gibberella; Hydrocarbons, Fluorinated; Microbial Sensitivity Tests; Oxidation-Reduction; Phenylhydrazines; Polyethylene Glycols; Pyrazoles; Structure-Activity Relationship; Thiocyanates; Thiourea; Tobacco Mosaic Virus

Desferrioxamine a well-known iron chelator was found to decrease hydrogen peroxide and phenylhydrazine induced lipid peroxidation of red blood cell membranes assessed by hydrocarbon gas release and loss of polyunsaturated fatty acids. Hydroxyl radical scavengers like mannitol and thiourea and proteins like albumin were unable to reduce peroxidative reactions to our system. Addition of uric acid (in an unphysiological concentration of 5 mM) to the incubation medium resulted in a slight reduction in H2O2/phenylhydrazine mediated break-down of arachidonic (20:4) and docosahexaenoic acid (22:6) in the erythrocyte membrane and consequently in a decreased alkane release and haemolysis.

Topics: Alkanes; Antioxidants; Deferoxamine; Erythrocytes; Fatty Acids; Free Radicals; Hemolysis; Humans; Mannitol; Phenylhydrazines; Thiourea

Topics: Air; Aminohydrolases; Azides; Copper; Cyanides; Hydrazines; Iproniazid; Liver; Manometry; Mitochondria; Monoamine Oxidase Inhibitors; Naphthoquinones; Nitrogen; Oxidoreductases; Oxygen; Phenylhydrazines; Research; Sarcina; Spectrophotometry; Thiourea; Tyramine