thiouracil and serinol

thiouracil has been researched along with serinol* in 1 studies

Other Studies

1 other study(ies) available for thiouracil and serinol

ArticleYear
Incorporation of Pseudo-complementary Bases 2,6-Diaminopurine and 2-Thiouracil into Serinol Nucleic Acid (SNA) to Promote SNA/RNA Hybridization.
    Chemistry, an Asian journal, 2020, Apr-17, Volume: 15, Issue:8

    Serinol nucleic acid (SNA) is a promising candidate for nucleic acid-based molecular probes and drugs due to its high affinity for RNA. Our previous work revealed that incorporation of 2,6-diaminpurine (D), which can form three hydrogen bonds with uracil, into SNA increases the melting temperature of SNA-RNA duplexes. However, D incorporation into short self-complementary regions of SNA promoted self-dimerization and hindered hybridization with RNA. Here we synthesized a SNA monomer of 2-thiouracil (sU), which was expected to inhibit base pairing with D by steric hindrance between sulfur and the amino group. To prepare the SNA containing D and sU in high yield, we customized the protecting groups on D and sU monomers that can be readily deprotected under acidic conditions. Incorporation of D and sU into SNA facilitated stable duplex formation with target RNA by suppressing the self-hybridization of SNA and increasing the stability of the heteroduplex of SNA and its complementary RNA. Our results have important implications for the development of SNA-based probes and nucleic acid drugs.

    Topics: 2-Aminopurine; Base Pairing; Hydrogen Bonding; Nucleic Acid Hybridization; Oligonucleotides; Phase Transition; Propanolamines; Propylene Glycols; RNA; Thiouracil; Transition Temperature

2020