thapsigargin has been researched along with lupenone* in 1 studies
1 other study(ies) available for thapsigargin and lupenone
| Article | Year |
|---|---|
Lupenone attenuates thapsigargin-induced endoplasmic reticulum stress and apoptosis in pancreatic beta cells possibly through inhibition of protein tyrosine kinase 2 activity.
Prolonged high levels of cytokines, glucose, or free fatty acids are associated with diabetes, elevation of cytosolic Ca. MIN6, INS-1, and native mouse islet cells were used. Western blot for protein expressions, measurement of [Ca. Thapsigargin increased the protein levels of cleaved caspase 3, cleaved PARP, and the phosphorylated form of JNK, ATF4, and CHOP. Thapsigargin increased the interaction between stromal interaction molecule1 (Stim1) and Orai1, enhancing store-operated calcium entry (SOCE). SOCE is further activated by protein tyrosine kinase 2 (Pyk2), which is Ca. These results suggested that lupenone attenuated thapsigargin-induced ER stress and apoptosis by inhibiting SOCE; this may be due to the hindrance of Pyk2-mediated Stim1 tyrosine phosphorylation. In beta cells that are inevitably exposed to frequent [Ca Topics: Animals; Apoptosis; Calcium; Cell Line; Diabetes Mellitus; Endoplasmic Reticulum Stress; Focal Adhesion Kinase 1; Focal Adhesion Kinase 2; Glucose; Insulin-Secreting Cells; Lupanes; Mice; Phosphorylation; Thapsigargin; Triterpenes; Tyrosine | 2023 |