thapsigargin and azasetron

To investigate the effects of a water extract of Hwangryunhaedok-tang (HHTE) on the pacemaker potentials of mouse interstitial cells of Cajal (ICCs).. We dissociated ICCs from small intestines and cultured. ICCs were immunologically identified using an anti-c-kit antibody. We used the whole-cell patch-clamp configuration to record the pacemaker potentials generated by cultured ICCs under the current clamp mode (. HHTE dose-dependently depolarized ICC pacemaker potentials. Pretreatment with a 5-HT. These results suggest that HHTE dose-dependently depolarizes ICC pacemaker potentials through 5-HT

Topics: Animals; Biological Clocks; Bridged Bicyclo Compounds, Heterocyclic; Calcium; Cells, Cultured; Drugs, Chinese Herbal; Enzyme Inhibitors; Gastrointestinal Motility; GTP-Binding Proteins; Interstitial Cells of Cajal; Intestine, Small; Membrane Potentials; Mice; Mice, Inbred ICR; Oxazines; Patch-Clamp Techniques; Phenols; Piperidines; Plant Extracts; Propane; Protein Kinase C; Receptors, Serotonin; rho-Associated Kinases; Serotonin 5-HT3 Receptor Antagonists; Serotonin 5-HT4 Receptor Antagonists; Sulfonamides; Thapsigargin