texas-red and 4-maleimidophenyl-isocyanate

High-density poly(ethylene glycol) (PEG) molecules are grafted onto Si surfaces in a brush-like configuration. We demonstrate that this surface is an excellent substrate for oligonucleotide immobilization. p-Maleimidophenyl isocyanate is used as a heterobifunctional cross-linker to tether thiol-modified oligonucleotides to terminal OH groups on the PEG brush. This approach gives excellent immobilization specificity and low background. The immobilized oligonucleotides show high sensitivity for the detection of complementary targets.

Topics: Base Sequence; Cross-Linking Reagents; Cyanates; Maleimides; Microscopy, Fluorescence; Nucleic Acid Hybridization; Oligonucleotides; Polyethylene Glycols; Sensitivity and Specificity; Silicon; Spectrometry, X-Ray Emission; Sulfhydryl Compounds; Surface Properties; Xanthenes