tetramethylrhodamine and silanediol

Ratiometric sensors generally couple binding events or chemical reactions at a distal site to changes in the fluorescence of a core fluorophore scaffold. However, such approaches are often hindered by spectral overlap of the product and reactant species. We provide a strategy to design ratiometric sensors that display dramatic spectral shifts by leveraging the chemoselective reactivity of novel functional groups inserted within fluorophore scaffolds. As a proof-of-principle, fluorophores containing a borinate (RF

Topics: Borinic Acids; Fluorescent Dyes; HeLa Cells; Humans; Microscopy, Confocal; Microscopy, Fluorescence; Molecular Structure; Rhodamines; Silanes; Xanthenes