tetramethylrhodamine and 2--deoxyadenosine-triphosphate

tetramethylrhodamine has been researched along with 2--deoxyadenosine-triphosphate* in 2 studies

Other Studies

2 other study(ies) available for tetramethylrhodamine and 2--deoxyadenosine-triphosphate

Article	Year
Identification of nucleotides with identical fluorescent labels based on fluorescence polarization in surfactant solutions. Analytical chemistry, 2001, Sep-15, Volume: 73, Issue:18 A solution-phase steady-state polarization-based method for discriminating among the four DNA nucleotides labeled identically with tetramethylrhodamine is described and demonstrated. Labeled nucleotides were dissolved in buffered surfactant solutions. In room temperature 4.5 mM Triton X-100 solutions at neutral pH, the measured steady-state polarizations of tetramethylrhodamine-labeled dATP, dCTP, dGTP and dUTP were 0.261 +/- 0.003, 0.112 +/- 0.003, 0.288 +/- 0.003, and 0.147 +/- 0.003, respectively. A blind test of 40 samples showed no errors in classification based on polarization. The reproducibility obtained during this study demonstrates that the four dye-labeled nucleotides can be discriminated with more than 99.8% confidence. Topics: Deoxyadenine Nucleotides; Deoxycytosine Nucleotides; Deoxyguanine Nucleotides; Deoxyuracil Nucleotides; Fluorescence Polarization; Fluorescent Dyes; Molecular Structure; Nucleotides; Octoxynol; Rhodamines; Solutions; Surface-Active Agents	2001
Internal fluorescence labeling with fluorescent deoxynucleotides in two-label peak-height encoded DNA sequencing by capillary electrophoresis. Nucleic acids research, 1994, Sep-25, Volume: 22, Issue:19 Fluorescently labeled deoxynucleotides were used for internal labeling of DNA sequencing fragments generated in a two-color peak-height encoded protocol. Sequenase and a manganese-containing buffer were used to generate uniform peak heights. Tetramethyl rhodamine - dATP was used in a labeling step, followed by termination with ddATP and ddCTP in a 3:1 ratio. Fluorescein - dATP was used in a second reaction, followed by termination with ddGTP and ddTTP in a 3:1 ratio. The fragments were pooled and separated by capillary gel electrophoresis. The results were compared with peak-height encoded sequencing based on fluorescently labeled primers. The dye-labeled primers produced higher resolution separations for shorter fragments. However, dye-labeled primer fragments suffered from an earlier onset of biased reptation and produced shorter sequencing reads. Fragments from 50 to at least 500 bases in length could be sequenced with the internal labels. Topics: Capillary Action; Deoxyadenine Nucleotides; Deoxyribonucleotides; Electrophoresis; Fluorescein; Fluoresceins; Fluorescent Dyes; Lasers; Rhodamines; Sequence Analysis, DNA; Spectrometry, Fluorescence	1994

Article

Year

Identification of nucleotides with identical fluorescent labels based on fluorescence polarization in surfactant solutions.

Analytical chemistry, 2001, Sep-15, Volume: 73, Issue:18

A solution-phase steady-state polarization-based method for discriminating among the four DNA nucleotides labeled identically with tetramethylrhodamine is described and demonstrated. Labeled nucleotides were dissolved in buffered surfactant solutions. In room temperature 4.5 mM Triton X-100 solutions at neutral pH, the measured steady-state polarizations of tetramethylrhodamine-labeled dATP, dCTP, dGTP and dUTP were 0.261 +/- 0.003, 0.112 +/- 0.003, 0.288 +/- 0.003, and 0.147 +/- 0.003, respectively. A blind test of 40 samples showed no errors in classification based on polarization. The reproducibility obtained during this study demonstrates that the four dye-labeled nucleotides can be discriminated with more than 99.8% confidence.

Topics: Deoxyadenine Nucleotides; Deoxycytosine Nucleotides; Deoxyguanine Nucleotides; Deoxyuracil Nucleotides; Fluorescence Polarization; Fluorescent Dyes; Molecular Structure; Nucleotides; Octoxynol; Rhodamines; Solutions; Surface-Active Agents

2001

Internal fluorescence labeling with fluorescent deoxynucleotides in two-label peak-height encoded DNA sequencing by capillary electrophoresis.

Nucleic acids research, 1994, Sep-25, Volume: 22, Issue:19

Fluorescently labeled deoxynucleotides were used for internal labeling of DNA sequencing fragments generated in a two-color peak-height encoded protocol. Sequenase and a manganese-containing buffer were used to generate uniform peak heights. Tetramethyl rhodamine - dATP was used in a labeling step, followed by termination with ddATP and ddCTP in a 3:1 ratio. Fluorescein - dATP was used in a second reaction, followed by termination with ddGTP and ddTTP in a 3:1 ratio. The fragments were pooled and separated by capillary gel electrophoresis. The results were compared with peak-height encoded sequencing based on fluorescently labeled primers. The dye-labeled primers produced higher resolution separations for shorter fragments. However, dye-labeled primer fragments suffered from an earlier onset of biased reptation and produced shorter sequencing reads. Fragments from 50 to at least 500 bases in length could be sequenced with the internal labels.

Topics: Capillary Action; Deoxyadenine Nucleotides; Deoxyribonucleotides; Electrophoresis; Fluorescein; Fluoresceins; Fluorescent Dyes; Lasers; Rhodamines; Sequence Analysis, DNA; Spectrometry, Fluorescence

1994