tetramethylrhodamine has been researched along with 1-2-dimyristoylphosphatidylethanolamine* in 1 studies
1 other study(ies) available for tetramethylrhodamine and 1-2-dimyristoylphosphatidylethanolamine
| Article | Year |
|---|---|
Evaluation of a high-content screening fluorescence-based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophages.
For understanding cholesterol and phospholipid efflux pathways there is a need for cellular fluorescence-based high-content screens (HCS) to investigated the cholesterol and phospholipid content in human macrophages.. Making use of fluorescence imaging based on HCS we have developed a tool to evaluate new agents that can act as inducers of cholesterol efflux. The fluorescence assay is based on the different staining patterns of cholesterol-loaded (E-LDL) and deloaded (HDL3) differentiated monocytes by the saturated, fluorescent lipid probe (1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine)-tetramethyl-rhodamin.. Morphologic examination and statistical evaluation of the staining pattern such as gray value, threshold area, shape factor and the spot size distribution provides evidence for a significant pattern change when cholesterol enriched and cholesterol depleted differentiated monocytes were imaged. Topics: Cell Membrane; Cholesterol; Fluorescent Dyes; Homeostasis; Humans; Image Cytometry; Lipid Metabolism; Lipoproteins, HDL; Lipoproteins, HDL3; Lipoproteins, LDL; Macrophages; Membrane Microdomains; Phosphatidylethanolamines; Reproducibility of Results; Rhodamines | 2006 |