tetracycline and resazurin

Topics: Ampicillin; Anti-Bacterial Agents; Electrodes; Escherichia coli; Kanamycin; Klebsiella pneumoniae; Microbial Sensitivity Tests; Oxazines; Platinum; Tetracycline; Xanthenes

To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants.. A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains.. This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts.

Topics: Anti-Bacterial Agents; Gentamicins; Indicators and Reagents; Microbial Sensitivity Tests; Oxazines; Reproducibility of Results; Streptococcus; Surface-Active Agents; Tetracycline; Xanthenes

The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measure Chlamydia infection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.

Topics: Animals; Anti-Bacterial Agents; Biological Assay; Cell Survival; Chlamydia Infections; Chlamydia trachomatis; Fibroblasts; Humans; Indicators and Reagents; L Cells; Mice; Microbial Sensitivity Tests; Oxazines; Polymyxin B; Tetracycline; Xanthenes