tetracycline and carbene

In this study, the roles of extracellular polymeric substances (EPSs) excreted by Pandoraea sp. XY-2 in the removal of tetracycline (TC) were investigated. In the early stage, TC in the solution was mainly removed by the adsorption of EPSs, which accounted for 20% of TC. Thereafter, large amount of TC was transported into the intracellular and biodegraded. EPSs was extracted and the contents of polyprotein and polysaccharides reached their maximum values (30.84 mg/g and 11.15 mg/g) in the first four days. Fourier transform infrared spectroscopy analysis revealed that hydroxyl, methylidyne, methylene and amide I groups in EPSs participated in the adsorption of TC. Furthermore, three-dimensional excitation-emission matrix fluorescence spectroscopy analysis revealed that TC caused the quenching of EPSs fluorescent groups. The quenching mechanism was attributed to static quenching and protein-like substances in EPSs from Pandoraea sp. XY-2 dominated the TC adsorption process. Bioinformatic analysis of Pandoraea sp. XY-2 genome identified multiple genes involved in exopolysaccharide synthesis and EPSs formation. The insights gained in this study might provide a better understanding about the adsorption process of EPSs in tetracycline-contaminated environment.

Topics: Adsorption; Anti-Bacterial Agents; Biotechnology; Burkholderiaceae; Extracellular Polymeric Substance Matrix; Methane; Polymers; Polyproteins; Polysaccharides; Sewage; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Tetracycline; Time Factors; Water Pollutants, Chemical; Water Purification

Topics: Anti-Bacterial Agents; Drug Therapy; Humans; Lymecycline; Lysine; Methane; Pharmacology; Respiratory Tract Infections; Tetracycline

Topics: Anti-Bacterial Agents; Biomedical Research; Female; Gynecology; Humans; Methane; Obstetrics; Oxytetracycline; Pharmacology; Pregnancy; Protein Synthesis Inhibitors; Tetracycline