tetracycline and 3-aminobenzeneboronic-acid

Significant concerns have been raised regarding to the pollution of antibiotics in recent years due to the abuse of antibiotics and their high detection rate in water. Herein, a novel super adsorbent, boronic acid-modified bacterial cellulose microspheres with a size of 415 μm in diameter was prepared through a facile water-in-oil emulsion method. The adsorbent was characterized by atomic force microscopy, scanning electron microscopy, and fourier transform infrared spectroscopy analyses to confirm its properties. The microspheres were applied as packing materials for the adsorption of tetracycline (TC) from an aqueous solution and hoggery sewer via the reversible covalent interaction between cis-diol groups in TC molecules and the boronic acid ligand. TC adsorption performance had been systemically investigated under various conditions, including the pH, temperature, TC concentration, contact time, and ionic strength. Results showed that the adsorption met pseudo-second-order, Elovich kinetic model and Sips, Redlich-Peterson isothermal models. And the adsorption process was spontaneous and endothermic, with the maximum TC adsorption capacity of 614.2 mg/g. After 18 adsorption-desorption cycles, the adsorption capacity remained as high as 84.5% compared with their original adsorption capacity. Compared with other reported adsorption materials, the microspheres had high adsorption capacity, a simple preparation process, and excellent recovery performance, demonstrating great potential in application on TC removal for water purification and providing new insights into the antibiotic's adsorption behavior of bacterial cellulose-based microspheres.

Topics: Adsorption; Anti-Bacterial Agents; Boronic Acids; Cellulose; Hydrogen-Ion Concentration; Kinetics; Microspheres; Tetracycline; Water; Water Pollutants, Chemical; Water Purification

A simple renewable surface for a rapid antibacterial susceptibility test has been demonstrated. The 3-aminophenylboronic acid (3-APBA) modified electrode bind with cis-diol groups on the cell wall of both gram positive and gram negative bacteria. The detection of antibacterial susceptibility response by a capacitive system can be done within a short time, 2.5h for the whole process, with good repeatability of the electrode's preparation. An acid solution, could break the bonding between 3-APBA and the bacteria, which were then easily removed by the fluid flow, renewing the sensing surface for the next test. This modified electrode can be reused up to 35 times. This sensor is useful for testing the susceptibility of bacteria to antibacterial agents that affect their cell wall. Results from the capacitive sensor corresponded well with the antimicrobial information in the literature and to the morphology of the treated bacteria revealed by scanning electron microscopy. Antimicrobial susceptibility to natural products could also be easily tested.

Topics: Ampicillin; Anti-Bacterial Agents; Biosensing Techniques; Boronic Acids; Ceftriaxone; Electrochemical Techniques; Electrodes; Gram-Negative Bacteria; Gram-Positive Bacteria; Humans; Microbial Sensitivity Tests; Microscopy, Electron, Scanning; Spectroscopy, Fourier Transform Infrared; Surface Properties; Tetracycline; Vancomycin; Xanthones