tetracarboxyphenylporphine and tris(2-carboxyethyl)phosphine

The uptake of the organophosphates tris(2-chloroethyl) phosphate (TCEP), tris(1-chloro-2-propyl) phosphate (TCPP), tributyl phosphate (TBP), the insect repellant N,N-diethyl toluamide (DEET), and the plasticizer n-butyl benzenesulfonamide (NBBS) into plants was studied in greenhouse experiments and simulated with a dynamic physiological plant uptake model. The calibrated model was coupled to a tipping buckets soil transport model and a field scenario with sewage sludge application was simulated. High uptake of the polar, low-volatile compounds TCEP, TCPP, and DEET into plants was found, with highest concentrations in straw (leaves and stem). Uptake into carrot roots was high for TCPP and TBP. NBBS showed no high uptake but was rapidly degraded. Uptake into barley seeds was small. The pattern and levels of uptake could be reproduced by the model simulations, which indicates mainly passive uptake and transport (i.e., by the transpiration stream, with the water) into and within the plants. Also the field simulations predicted a high uptake from soil into plants of TCEP, TCPP, and DEET, while TBP is more likely taken up from air. The BCF values measured and calculated in the greenhouse study are in most cases comparable to the calculated values of the field scenario, which demonstrates that greenhouse studies can be suitable for predicting the behavior of chemicals in the field. Organophosphates have a high potential for bioaccumulation in crops and reach agricultural fields both via sewage sludge and by atmospheric deposition.

Topics: Chemical Phenomena; Daucus carota; DEET; Environmental Monitoring; Environmental Pollutants; Hordeum; Models, Biological; Organophosphates; Phosphines; Porphyrins; Risk Assessment; Rivers; Seeds; Sewage; Sulfonamides

Organophosphate flame retardants (OPFRs) are used as replacements for the commercial PentaBDE mixture that was phased out in 2004. OPFRs are ubiquitous in the environment and detected at high concentrations in residential dust, suggesting widespread human exposure. OPFRs are structurally similar to neurotoxic organophosphate pesticides, raising concerns about exposure and toxicity to humans. This study evaluated the neurotoxicity of tris (1,3-dichloro-2-propyl) phosphate (TDCPP) compared to the organophosphate pesticide, chlorpyrifos (CPF), a known developmental neurotoxicant. We also tested the neurotoxicity of three structurally similar OPFRs, tris (2-chloroethyl) phosphate (TCEP), tris (1-chloropropyl) phosphate (TCPP), and tris (2,3-dibromopropyl) phosphate (TDBPP), and 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), a major component of PentaBDE. Using undifferentiated and differentiating PC12 cells, changes in DNA synthesis, oxidative stress, differentiation into dopaminergic or cholinergic neurophenotypes, cell number, cell growth and neurite growth were assessed. TDCPP displayed concentration-dependent neurotoxicity, often with effects equivalent to or greater than equimolar concentrations of CPF. TDCPP inhibited DNA synthesis, and all OPFRs decreased cell number and altered neurodifferentiation. Although TDCPP elevated oxidative stress, there was no adverse effect on cell viability or growth. TDCPP and TDBPP promoted differentiation into both neuronal phenotypes, while TCEP and TCPP promoted only the cholinergic phenotype. BDE-47 had no effect on cell number, cell growth or neurite growth. Our results demonstrate that different OPFRs show divergent effects on neurodifferentiation, suggesting the participation of multiple mechanisms of toxicity. Additionally, these data suggest that OPFRs may affect neurodevelopment with similar or greater potency compared to known and suspected neurotoxicants.

Topics: Animals; Cell Culture Techniques; Cell Survival; Choline O-Acetyltransferase; DNA; Flame Retardants; Nerve Tissue Proteins; Organophosphates; Organophosphorus Compounds; Oxidative Stress; PC12 Cells; Phosphines; Porphyrins; Rats; Tyrosine 3-Monooxygenase

The organophosphorus esters tris-(2-chloroethyl)-phosphate (TCEP) and tris-(2-chloropropyl)-phosphate (TCPP) have been widely used as flame retardants and fire preventing agents, e.g. in polyurethane foams. We investigated the cytotoxic, genotoxic, mutagenic, and estrogenic potentials of TCEP and TCPP, using different in vitro models. Cytotoxic effects were evaluated by neutral red uptake and genotoxicity with the alkaline single cell gel electrophoresis (Comet assay), both in V79 (hamster fibroblasts) cells. Mutagenicity was tested in the Ames assay with Salmonella typhimurium using the strains TA 97 a, 98, 100, 102, 104, 1535, 1537, and 1538, with and without metabolic activation by S9-rat liver homogenate. Estrogenic or anti-estrogenic effects were examined with the recombinant yeast reporter gene assay, and in human endometrial cancer Ishikawa cells by induction of alkaline phosphatase. In V79 cells TCEP was weakly cytotoxic at concentrations above 10 microM in the presence of S9-rat liver homogenate whereas TCPP showed cytotoxicity above 1mM in the presence of S9. Both substances did not induce DNA strand breaks in the alkaline version of the Comet assay neither without an external enzymatic metabolizing system, nor in the presence of S9-mix. Additionally, no mutagenic potential could be detected for TCEP and TCPP in eight Salmonella strains using concentrations up to 1mM in the presence and absence of S9. Hormonal activity shown as induction of estrogenic or anti-estrogenic effects could not be detected in the two in vitro test systems.

Topics: Animals; Cell Line; Cytotoxins; DNA Damage; Dose-Response Relationship, Drug; Estrogens; Humans; Liver; Mutagenicity Tests; Mutagens; Phosphines; Porphyrins; Rats; Saccharomyces cerevisiae; Salmonella typhimurium