telocinobufagin and marinobufagenin

telocinobufagin has been researched along with marinobufagenin* in 5 studies

Other Studies

5 other study(ies) available for telocinobufagin and marinobufagenin

ArticleYear
Telocinobufagin, a Novel Cardiotonic Steroid, Promotes Renal Fibrosis via Na⁺/K⁺-ATPase Profibrotic Signaling Pathways.
    International journal of molecular sciences, 2018, Aug-29, Volume: 19, Issue:9

    Cardiotonic steroids (CTS) are Na⁺/K⁺-ATPase (NKA) ligands that are elevated in volume-expanded states and associated with cardiac and renal dysfunction in both clinical and experimental settings. We test the hypothesis that the CTS telocinobufagin (TCB) promotes renal dysfunction in a process involving signaling through the NKA α-1 in the following studies. First, we infuse TCB (4 weeks at 0.1 µg/g/day) or a vehicle into mice expressing wild-type (WT) NKA α-1, as well as mice with a genetic reduction (~40%) of NKA α-1 (NKA α-1

    Topics: Animals; Bufanolides; Cell Line; Fibrosis; Glycogen Synthase Kinase 3 beta; Kidney Diseases; MAP Kinase Signaling System; Mice; Ouabain; Phosphorylation; Signal Transduction; Sodium-Potassium-Exchanging ATPase; Swine

2018
Telocinobufagin and Marinobufagin Produce Different Effects in LLC-PK1 Cells: A Case of Functional Selectivity of Bufadienolides.
    International journal of molecular sciences, 2018, Sep-14, Volume: 19, Issue:9

    Topics: Animals; Apoptosis; Bufanolides; Cell Cycle; Cell Proliferation; Cell Survival; Glycogen Synthase Kinase 3 beta; LLC-PK1 Cells; MAP Kinase Signaling System; Sodium-Potassium-Exchanging ATPase; Swine; Wnt Signaling Pathway

2018
Inhibitory effect of combinations of digoxin and endogenous cardiotonic steroids on Na+/K+-ATPase activity in human kidney membrane preparation.
    Life sciences, 2011, Jan-03, Volume: 88, Issue:1-2

    Cardiac glycosides have been extensively used in the treatment of congestive heart failure for more than 200 years. Recently, cardenolides and bufadienolides were isolated from mammalian tissue and are considered as a new class of steroidal hormones. The aim of the present work was to characterize the interaction between the most clinical used cardiac glycoside digoxin and the cardiac glycosides known to exist endogenously, i.e., ouabain, marinobufagin and telocinobufagin, on human kidney Na(+)/K(+)-ATPase.. Inhibition of Na(+)/K(+)-ATPase activity from crude membrane preparations of human kidney was performed using increasing concentrations of the drugs alone or mixtures of ouabain:digoxin, telocinobufagin:digoxin and marinobufagin:digoxin in a fixed ratio 1:4, 2:3 and 3:2, respectively. The colorimetric method of Fiske and Subbarow was used to measure the inorganic phosphate released.. Analyses of inhibition curves showed that the experimental curves for all combinations were superimposed on the theoretical additive curves indicating that an additive effect occurs among distinct cardenolides and bufadienolides combinations on the human α1β1 Na(+)/K(+)-ATPase protomer.. Considering the extensive use of digoxin in the treatment of heart failure and the recent findings that endogenous cardiac glycosides may have altered levels in many diseases, including heart failure, the demonstration of additive effect between cardiac glycosides can help in the understanding of recent clinical observations, including that lower than usual doses of cardiac glycosides are necessary for decreasing mortality in these patients.

    Topics: Bufanolides; Cardiac Glycosides; Cell Membrane; Digoxin; Dose-Response Relationship, Drug; Drug Interactions; Humans; Inhibitory Concentration 50; Isoenzymes; Kidney; Ouabain; Sodium-Potassium-Exchanging ATPase

2011
A novel endogenous digitalis, telocinobufagin, exhibits elevated plasma levels in patients with terminal renal failure.
    Clinical biochemistry, 2005, Volume: 38, Issue:1

    There are several potential endogenous digitalis-like factors (EDLF) in mammalian body fluids, and marinobufagenin (MBG) may be the most potent EDLF. Improved assays are needed to confirm the potency of these metabolites. In the present study, we have identified MBG and telocinobufagin (TCB) in human plasma by high-resolution mass spectrometry (MS) and nuclear magnetic resonance (NMR).. The high-resolution MS analysis revealed the molecular masses of TCB and MBG to be the same as their respective theoretical values. Using a tandem mass spectrometer, the mass-charge ratio for TCB was determined to be 403.2 for the parent ion and 349.2 for the daughter ion. The mass-charge ratio for MBG was m/z 383.2 and m/z 401.2. The NMR study revealed that the signals for MBG and TCB were the same as those obtained by MS analysis. In human blood, MBG and TCB were also identified by liquid chromatography (LC) as well as MS. In the LC/MS assay, proscillaridin A was used as an internal standard. The plasma was pretreated with Sep-Pak C18, and then 50 microL was applied to the C8 high-performance liquid chromatography (HPLC) column. The mean plasma concentration of MBG in healthy volunteers (0.94 +/- 0.28 ng/mL) was significantly lower than that in patients undergoing regular hemodialysis (3.81 +/- 1.92 ng/mL). The concentration of TCB in the healthy volunteers (1.80 +/- 0.55 ng/mL) was also significantly lower than that in patients with terminal renal failure (6.86 +/- 4.30 ng/mL).. These results indicate that the major EDLF is TCB because its plasma concentration is the highest among the reported endogenous digitalis candidates.

    Topics: Bufanolides; Carbon Isotopes; Humans; Hydrogen; Kidney Failure, Chronic; Magnetic Resonance Spectroscopy; Mass Spectrometry; Middle Aged; Time Factors

2005
Antimicrobial activity of the bufadienolides marinobufagin and telocinobufagin isolated as major components from skin secretion of the toad Bufo rubescens.
    Toxicon : official journal of the International Society on Toxinology, 2005, Volume: 45, Issue:6

    The increase in the emergence of antibiotic-resistant microorganisms and difficult to treat infections caused by these pathogens stimulate research aiming the identification of novel antimicrobials. Skin secretion of amphibian contains a large number of biologically active compounds, including compounds that performance defense mechanisms against microorganisms. In the present work, two antimicrobial bufadienolides, telocinobufagin (402.1609 Da) and marinobufagin (400.1515 Da), were isolated from skin secretions of the Brazilian toad Bufo rubescens. The specimens were collected in Brasilia (Distrito Federal, Brazil), the skin secretions extracted by electric stimulation, and submitted to purification by RP-HPLC. The molecular structure and mass determination were done by (1)H and (13)C NMR and mass spectrometry data, respectively. The antimicrobial activity was performed by liquid growth inhibition against Staphylococcus aureus and Escherichia coli. The minimum inhibitory concentrations of telocinobufagin and marinobufagin were, respectively, 64.0 and 16.0 microg/mL for E. coli and both 128 microg/mL for S. aureus. Besides the antimicrobial activity both bufadienolides promoted an increase of the contraction force in isolated frog ventricle strips.

    Topics: Amphibian Venoms; Animals; Anti-Bacterial Agents; Brazil; Bufanolides; Bufonidae; Cardiotonic Agents; Chromatography, High Pressure Liquid; Escherichia coli; Magnetic Resonance Spectroscopy; Mass Spectrometry; Microbial Sensitivity Tests; Myocardial Contraction; Protein Conformation; Skin; Staphylococcus aureus

2005