tellurium and phosphoramidite

tellurium has been researched along with phosphoramidite* in 3 studies

Other Studies

3 other study(ies) available for tellurium and phosphoramidite

ArticleYear
Synthesis, structure and imaging of oligodeoxyribonucleotides with tellurium-nucleobase derivatization.
    Nucleic acids research, 2011, Volume: 39, Issue:9

    We report here the first synthesis of 5-phenyl-telluride-thymidine derivatives and the Te-phosphoramidite. We also report here the synthesis, structure and STM current-imaging studies of DNA oligonucleotides containing the nucleobases (thymine) derivatized with 5-phenyl-telluride functionality (5-Te). Our results show that the 5-Te-DNA is stable, and that the Te-DNA duplex has the thermo-stability similar to the corresponding native duplex. The crystal structure indicates that the 5-Te-DNA duplex structure is virtually identical to the native one, and that the Te-modified T and native A interact similarly to the native T and A pair. Furthermore, while the corresponding native showed weak signals, the DNA duplex modified with electron-rich tellurium functionality showed strong topographic and current peaks by STM imaging, suggesting a potential strategy to directly image DNA without structural perturbation.

    Topics: Crystallography, X-Ray; DNA; Microscopy, Scanning Tunneling; Models, Molecular; Nucleic Acid Denaturation; Oligodeoxyribonucleotides; Organophosphorus Compounds; Tellurium; Thymidine

2011
Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.
    Current protocols in nucleic acid chemistry, 2011, Volume: Chapter 1

    In this unit, an efficient method for the synthesis of 2'-tellerium-modified phosphoramidite and its incorporation into oligonucleotide are presented. We choose 5'-O-DMTr-2,2'-anhydro-uridine and -thymidine nucleosides (S.1, S.2) as starting materials due to their easy preparation. The 5'-O-DMTr-2,2'-anhydro-uridine and -thymidine can be converted to the corresponding 2'-tellerium-derivatized nucleosides by treating with the telluride nucleophiles. Subsequently, the 2'-Te-nucleosides can be transformed into 3'-phosphoramidites, which are the building blocks for DNA/RNA synthesis. The DNA synthesis, purification, and applications of oligonucleotides containing 2'-Te-U or 2'-Te-T are described in the protocol.

    Topics: DNA; Models, Chemical; Molecular Structure; Oligodeoxyribonucleotides; Organometallic Compounds; Organophosphorus Compounds; Tellurium

2011
Synthesis of the first tellurium-derivatized oligonucleotides for structural and functional studies.
    Chemistry (Weinheim an der Bergstrasse, Germany), 2009, Oct-05, Volume: 15, Issue:39

    We report here the first synthesis of Te-nucleoside phosphoramidites and Te-modified oligonucleotides. We protected the 2'-tellurium functionality by alkylation and found that the Te functionality is compatible with solid-phase synthesis and that the Te oligonucleotides are stable during deprotection and purification. In addition, the redox properties of the Te functionalities have been explored. We found that the telluride and telluoxide DNAs are interchangeable by redox reactions. At elevated temperature, the Te-DNA can also be site-specifically fragmented oxidatively or reductively when 2'-TePh functionality is present, whereas elimination of the nucleobase is observed in the presence of 2'-TeMe. Moreover, the stability of the DNA duplexes derivatized with the Te functionalities has been investigated. Our Te derivatization of nucleic acids provides a novel approach for investigating DNA damage as well as for structure and function studies of nucleic acids and their protein complexes.

    Topics: Catalysis; Cross-Linking Reagents; Crystallography, X-Ray; DNA; DNA Damage; Molecular Sequence Data; Molecular Structure; Nucleic Acid Conformation; Oligonucleotides; Organophosphorus Compounds; Spectrophotometry, Ultraviolet; Tellurium; Temperature

2009