tellurium and deoxynivalenol

Deoxynivalenol (DON) is considered a mycotoxin that is toxic to the agricultural environment and human body. It is necessary to study the pathophysiological mechanism of DON toxicity at the cellular level. Cytochrome c (Cyt c), as an important biomarker of DON-induced apoptosis that may lead to a bipartite 'point-of-no return' event, is of great significance to be detected using cell imaging. Herein, we synthesized a DON-deactivated emission fluorescent probe, the molecularly imprinted polymer-coated quantum dots (CdTe@MIP), for monitoring the Cyt c level with a photoinduced electron transfer strategy. The CdTe@MIP probe can be easily loaded into cells and perform well due to its great sensitivity and selectivity and its fluorescence was gradually quenched with the increasing concentration (0-10 μM) and incubation time (0-7.5 h) of DON. Cell imaging results of apoptosis induced by DON was consistent with that of the cell counting kit-8 assay and flow cytometry technique. The developed method can be used to monitor DON-induced apoptosis and provide an early-warning system for the contaminant toxicity.

Topics: Apoptosis; Cadmium Compounds; Cytochromes c; Electrons; Fluorescent Dyes; Humans; Quantum Dots; Tellurium; Trichothecenes