tauropine has been researched along with strombine* in 2 studies
2 other study(ies) available for tauropine and strombine
| Article | Year |
|---|---|
Improved HPLC determination of acidic opines by phenylisothiocyanate derivatization and its application to marine animals.
We present here a reliable and sensitive method for the determination of acidic opines such as meso-alanopine, beta-alanopine, tauropine and strombine in biological samples. Interfering primary amino acids were eliminated by reaction with o-phthalaldehyde, and the derivatized compounds were passed through Sep-Pak Plus PS-1 cartridges. The acidic opines were recovered by flushing the cartridges with water, then determined by high performance liquid chromatography after a second derivatization with phenylisothiocyanate. All 4 acidic opines were detected within 30 min. This method ensured good separation and guaranteed almost full recovery of all acidic opines. This method was applied to analyze opines in marine animals and to test whether opines are metabolized in the livers of the rat and fish. Topics: Alanine; Amino Acids, Sulfur; Animals; Calibration; Chromatography, High Pressure Liquid; Fishes; Male; Rats; Rats, Wistar; Sarcosine | 2000 |
Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization.
A new method for the quantitative analysis of acidic opines--alanopine, strombine, tauropine, and beta-alanopine--is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines after partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, 10-30 pmol minimum detection, and better reproducibility than the high-voltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of beta-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method. Topics: Alanine; Amino Acids, Sulfur; Animals; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Glycine; Indicators and Reagents; Isothiocyanates; Mollusca; Sarcosine; Thiocyanates | 1988 |