tacrolimus and ifenprodil

Whole-cell recording in the superficial layers of the developing superior colliculus (sSC) reveals a large drop in NMDA receptor (NMDAR) current decay time synchronized across all neurons and occurring consistently between P10 and P11. We show that blocking the Ca2+/calmodulin-dependent phosphatase calcineurin (CaN) in the postsynaptic neuron can abolish this drop. The regulation is induced prematurely by 1-2 hr of electrical stimulation in P10 collicular slices only if CaN and NMDAR currents can be activated in the neuron. These data suggest that a long-lasting, CaN-mediated control of NMDAR kinetics is rapidly initiated by heightened activity of the NMDAR itself and demonstrate a novel developmental and tonic function of CaN that can play an important role in modulating the plasticity of the developing CNS.

Topics: Action Potentials; Calcineurin; Calcineurin Inhibitors; Dose-Response Relationship, Drug; Down-Regulation; Electric Stimulation; Enzyme Inhibitors; Excitatory Amino Acid Antagonists; Excitatory Postsynaptic Potentials; Gene Expression Regulation, Developmental; In Vitro Techniques; Ion Transport; Neuronal Plasticity; Neurons; Patch-Clamp Techniques; Piperidines; Receptors, N-Methyl-D-Aspartate; Superior Colliculi; Synapses; Tacrolimus