tachyplesin-peptide--tachypleus-tridentatus and plerixafor

T22 ([Tyr5,12, Lys7]-polyphemusin II) is an 18-residue peptide amide, which has strong anti-HIV activity. T22 inhibits the T cell line-tropic (T-tropic) HIV-1 infection through its specific binding to a chemokine receptor CXCR4, which serves as a coreceptor for the entry of T-tropic HIV-1 strains. Herein, we report our finding of novel 14-residue CXCR4 inhibitors, T134 and T140, on the basis of the T22 structure. In the assays we examined, T140 showed the highest inhibitory activity against HIV-1 entry and the strongest inhibitory effect on the binding of an anti-CXCR4 monoclonal antibody (12G5) to CXCR4 among all the CXCR4 inhibitors that have been reported up to now.

Topics: Amino Acid Sequence; Anti-HIV Agents; Antimicrobial Cationic Peptides; Benzylamines; Cells, Cultured; Chemokine CXCL12; Chemokines, CXC; Circular Dichroism; Cyclams; DNA-Binding Proteins; Heterocyclic Compounds; HIV-1; Molecular Sequence Data; Oligopeptides; Peptides; Peptides, Cyclic; Receptors, CXCR4; T-Lymphocytes