syringaldazine and sinapinic-acid

syringaldazine has been researched along with sinapinic-acid* in 2 studies

Other Studies

2 other study(ies) available for syringaldazine and sinapinic-acid

ArticleYear
Prediction model based on decision tree analysis for laccase mediators.
    Enzyme and microbial technology, 2013, Jan-10, Volume: 52, Issue:1

    A Structure Activity Relationship (SAR) study for laccase mediator systems was performed in order to correctly classify different natural phenolic mediators. Decision tree (DT) classification models with a set of five quantum-chemical calculated molecular descriptors were used. These descriptors included redox potential (ɛ°), ionization energy (E(i)), pK(a), enthalpy of formation of radical (Δ(f)H), and OH bond dissociation energy (D(O-H)). The rationale for selecting these descriptors is derived from the laccase-mediator mechanism. To validate the DT predictions, the kinetic constants of different compounds as laccase substrates, their ability for pesticide transformation as laccase-mediators, and radical stability were experimentally determined using Coriolopsis gallica laccase and the pesticide dichlorophen. The prediction capability of the DT model based on three proposed descriptors showed a complete agreement with the obtained experimental results.

    Topics: Acetophenones; Benzaldehydes; Biocatalysis; Catechols; Coumaric Acids; Decision Trees; Dichlorophen; Fungal Proteins; Hydrazones; Laccase; Models, Chemical; Models, Molecular; Molecular Structure; Nitrophenols; Oxidation-Reduction; Phenols; Polyporales; Protein Conformation; Quantitative Structure-Activity Relationship; Vanillic Acid

2013
Purification and characterization of the constitutive form of laccase from the basidiomycete Coriolus hirsutus and effect of inducers on laccase synthesis.
    Biotechnology and applied biochemistry, 1998, Volume: 28, Issue:1

    An isolate of Coriolus hirsutus constitutively expresses substantial amounts of extracellular laccase on a defined growth medium. The most efficient inducer of extracellular laccase synthesis was syringaldazine, which increased the enzyme yield by 1000% at a concentration of 0.11 microM. The constitutive form of the enzyme was purified 312-fold. Laccase from C. hirsutus, with an estimated molecular mass of 55 kDa and pI of 4.0, is a monomeric glycoprotein containing 12% carbohydrate consisting of mannose and N-acetylglucosamine. The laccase was found to contain 3.9-4.1 copper atoms per molecule. The absorption spectrum shows a maximum at 610 nm and a shoulder at 330 nm, which is typical of laccase possessing type 1 and type 3 copper atoms. The parameters of the first type of copper were determined by EPR as g perpendicular=2.046 and g parallel=2.200, A parallel=8.103 x 10(-3) cm-1. Laccase was found to be a pH-stable and thermostable enzyme. With organic substrates it exhibits a pH optimum of 4.5, but with the inorganic substrate K4[Fe(CN)6] this decreased to 3.5. The highest efficiency of catalysis was observed with sinapinic acid as the substrate. The kinetic constants kcat and Km of this reaction were 578 s-1 and 24 microM respectively. It was established that the kinetics of the assayed reaction shows a Ping Pong mechanism.

    Topics: Amino Acids; Basidiomycota; Coumaric Acids; Electron Spin Resonance Spectroscopy; Enzyme Induction; Fungal Proteins; Glycoproteins; Hydrazones; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Laccase; Metalloproteins; Oxidoreductases; Spectrophotometry; Substrate Specificity

1998