succinyladenosine and phenylsulfate

To identify blood markers for early stages of chronic kidney disease (CKD), blood samples were collected from rats with adenine-induced CKD over 28 days. Plasma samples were subjected to metabolomic profiling by liquid chromatography-mass spectrometry, followed by multivariate analyses. In addition to already-identified uremic toxins, we found that plasma concentrations of N6-succinyl adenosine, lysophosphatidylethanolamine 20:4, and glycocholic acid were altered, and that these changes during early CKD were more sensitive markers than creatinine concentration, a universal indicator of renal dysfunction. Moreover, the increase in plasma indoxyl sulfate concentration occurred earlier than increases in phenyl sulfate and p-cresol sulfate. These novel metabolites may serve as biomarkers in identifying early stage CKD.

Topics: Adenine; Adenosine; Animals; Biomarkers; Chromatography, Liquid; Cresols; Early Diagnosis; Glycocholic Acid; Indican; Kidney Failure, Chronic; Lysophospholipids; Male; Metabolomics; Multivariate Analysis; Rats; Rats, Sprague-Dawley; Sulfuric Acid Esters; Tandem Mass Spectrometry