strychnine and 3-hydroxyaspartic-acid

Signaling by glutamatergic synapses plays an important role in visual processing in the retina. In this study, we used an enzyme-linked fluorescence assay system to monitor the dynamics of extracellular glutamate in a slice preparation from the mouse retina. High K stimulation induced an elevation of fluorescence in the inner plexiform layer (IPL) of the retina when glutamate transporters were inhibited by dl-threo-β-benzyloxyaspartic acid (TBOA). The high K-induced fluorescence signals in the IPL were inhibited by the calcium channel blocker Cd

Topics: Animals; Aspartic Acid; Cadmium Compounds; Calcium Channel Blockers; Enzyme-Linked Immunosorbent Assay; GABA Antagonists; Glutamic Acid; Mice; Mice, Inbred C57BL; Picrotoxin; Potassium; Retina; Retinal Ganglion Cells; Spectrometry, Fluorescence; Strychnine; Synapses; Vesicular Glutamate Transport Proteins