stilbenes and n-hexane

stilbenes has been researched along with n-hexane* in 4 studies

Other Studies

4 other study(ies) available for stilbenes and n-hexane

ArticleYear
Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC.
    Journal of chromatographic science, 2016, Volume: 54, Issue:3

    High efficiency and less solvent consumption are the essential requirements of high-speed countercurrent chromatography (HSCCC), especially for the large-scale preparation. In this study, an efficient HSCCC strategy with consecutive sample injection was successfully developed to rapidly separate and purify rhaponticin and rhapontigenin from the seeds of the Chinese medicinal herb fenugreek (Trigonella foenum-graecum L.). The effective separation was achieved using n-hexane-ethyl acetate-methanol-water (1:4:2:6, v/v/v/v) as the two-phase solvent system, in which the mobile phase was eluted at an optimized flow rate of 2.2 mL/min and a revolution speed of 850 rpm. After consecutively loading four identical fenugreek samples, each containing 120 mg, HSCCC separation yielded 146.4 mg of rhaponticin and 174.8 mg of rhapontigenin with purities of 98.6 and 99.1%, respectively, as determined by high-performance liquid chromatography at 320 nm. Their chemical structures were identified using UV spectroscopy, (1)H-NMR and (13)C-NMR. The HSCCC method with consecutive sample injection allowed faster separation and produced less solvent waste, suggesting that it is an efficient way to rapidly separate and purify natural products on a large scale.

    Topics: Acetates; Chromatography, High Pressure Liquid; Countercurrent Distribution; Flow Injection Analysis; Hexanes; Liquid-Liquid Extraction; Methanol; Plant Extracts; Seeds; Solvents; Stilbenes; Trigonella; Water

2016
Preparative isolation and purification of antioxidative stilbene oligomers from Vitis chunganeniss using high-speed counter-current chromatography in stepwise elution mode.
    Journal of separation science, 2009, Volume: 32, Issue:14

    Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of three stilbene oligomers from Vitis chunganeniss using stepwise elution with a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water at (2:5:2:5, v/v) and (1:2:1:2, v/v). The preparative HSCCC separation was performed on 800 mg of crude sample yielding hopeaphenol (21.1 mg), amurensin G (37.2 mg) and vitisin A (95.6 mg) in a one-step separation, with purities over 95% as determined by HPLC. The structures of these three compounds were identified by MS, (1)H NMR and (13)C NMR. In addition, their antioxidant activities were screened by DPPH assay, where vitisin A showed strong antioxidant activity. Further EPR experiments with spin-trapping technique demonstrated that vitisin A is a potent and selective singlet oxygen quencher, which may be used in singlet oxygen-mediated diseases as a pharmacological agent.

    Topics: Acetates; Antioxidants; Chromatography, High Pressure Liquid; Countercurrent Distribution; Hexanes; Methanol; Molecular Conformation; Solvents; Stilbenes; Time Factors; Vitis; Water

2009
[Some derivatives of 3,4-di-(p-methoxy)phenyl-hexane: chemical and pharmacological study].
    Comptes rendus hebdomadaires des seances de l'Academie des sciences, 1958, Jul-28, Volume: 247, Issue:4

    Topics: Amines; Hexanes; Ketones; Stilbenes

1958
[Amino alcohol derivatives of 3,4-bis-(p-methoxyphenyl)hexane; chemical & pharmacological study].
    Comptes rendus hebdomadaires des seances de l'Academie des sciences, 1958, Aug-04, Volume: 247, Issue:5

    Topics: Amino Alcohols; Ammonium Compounds; Hexanes; Quaternary Ammonium Compounds; Research; Stilbenes

1958