stigmasterol and poriferasterol-monoglucoside

6:2 chlorinated polyfluorinated ether sulfonate (F-53B) has been widely applied as a mist suppressant to replace perfluorooctane sulfonate (PFOS) in the metal plating industry in China for decades. Recently, F-53B has been frequently identified in the aquatic environment and wild-caught fish. However, studies on the uptake and elimination kinetics, and the toxicological effects of F-53B were very scarce. In this study, zebrafish larvae (72 h post fertilization, hpf) were exposed to F-53B (10, 100 μg/L) for 48 h, followed by a 24 h of depuration to examine both the dynamics of accumulation and elimination of F-53B and responses of antoxidant defense system in fish. The results showed that F-53B rapidly accumulated in zebrafish larvae in a concentration and time-dependent manner with BCF values of 3612-3615, but was eliminated slowly (half-life ranged from 241.5 to 258.6 h). F-53B exposure induced oxidative stress in zebrafish larvae, as reflected by the reduction in the GSH and MDA contents, CAT, SOD, CuZn-SOD, and GSH-ST activities, and the increase in GSH-Px activity as well as CAT and SOD protein levels. However, these oxidative stress markers were restored to control levels except for a decrease in protein level of SOD after depuration. Collectively, the results of this work indicate that F-53B behaves like PFOS and is bioaccumulative and persistent in zebrafish larvae, and further induced oxidative stress responses.

Topics: Alkanesulfonates; Alkanesulfonic Acids; Animals; Biomarkers; China; Fluorocarbons; Larva; Oxidative Stress; Stigmasterol; Superoxide Dismutase; Water Pollutants, Chemical; Zebrafish

A glycolipid which was expressed during a differentiation from haploid myxoamoebae to diploid plasmodia of a true slime mold, Physarum polycephalum, has been examined. In the amoeboid stage, cells did not contain this glycolipid, but after conjugation of the haploid cells, this substance appeared and increased in its amount. From structural studies of the purified glycolipid, it has been identified as poriferasterol monoglucoside.

Topics: Cell Differentiation; Chemical Phenomena; Chemistry, Physical; Chromatography, Thin Layer; Glycolipids; Physarum; Phytosterols; Stigmasterol

Topics: Charcoal; Chemistry, Pharmaceutical; Cortisone; Culture Media; Pharmacy; Research; Stigmasterol

Topics: Blood Proteins; Colchicine; Skin Neoplasms; Stigmasterol

Topics: Colchicine; Skin; Stigmasterol

Topics: Colchicine; Radiotherapy; Skin Diseases; Stigmasterol

Topics: Administration, Cutaneous; Blood Proteins; Colchicine; Electrophoresis; Humans; Stigmasterol; Syphilis; Syphilis, Cutaneous

Topics: Antimitotic Agents; Colchicine; Female; Humans; Neoplasms; Stigmasterol; Uterine Cervical Neoplasms; Vulvar Neoplasms

Topics: Animals; Colchicine; Erythrocytes; Lagomorpha; Poisoning; Rabbits; Stigmasterol

Topics: Chemistry Techniques, Analytical; Crystallization; Crystallography; Stigmasterol

Topics: Alkaloids; Animals; Antimitotic Agents; Colchicine; Colchicum; Neoplasms, Experimental; Stigmasterol

Topics: Colchicine; Colchicum; Humans; Leukocytes; Stigmasterol